Phospholipid metabolism in the brown alga, Fucus serratus

Smith, Kim I.; Douce, Roland; Harwood, John L.

doi:10.1016/0031-9422(82)83142-7

Cited by 48 publications

(20 citation statements)

References 15 publications

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“…Pawlik-Skowronska et al 2007). During such maintenance, untreated algae maintained their full viability as evaluated by growth, photosynthesis and metabolism Smith et al 1982).…”

Section: Algal Exposuresmentioning

confidence: 99%

Induction of expression of a 14-3-3 gene in response to copper exposure in the marine alga, Fucus vesiculosus

et al. 2011

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The macro-alga Fucus vesiculosus has a broad global and estuarine distribution and exhibits exceptional resistance to toxic metals, the molecular basis of which is poorly understood. To address this issue a cDNA library was constructed from an environmental isolate of F. vesiculosus growing in an area with chronic copper pollution. Characterisation of this library led to the identification of a cDNA encoding a protein known to be synthesised in response to toxicity, a full length 14-3-3 exhibiting a 71% identity to human/mouse epsilon isoform, 70-71% identity to yeast BMH1/2 and 95 and 71% identity to the Ectocarpus siliculosus 14-3-3 isoforms 1 and 2 respectively. Preliminary characterisation of the expression profile of the 14-3-3 indicated concentration- and time-dependent inductions on acute exposure of F. vesiculosus of copper (3-30 μg/l). Higher concentrations of copper (≥150 μg/l) did not elicit significant induction of the 14-3-3 gene compared with the control even though levels of both intracellular copper and the expression of a cytosolic metal chaperone, metallothionein, continued to rise. Analysis of gene expression within environmental isolates demonstrated up-regulation of the 14-3-3 gene associated with the known copper pollution gradient. Here we report for the first time, identification of a gene encoding a putative 14-3-3 protein in a multicellular alga and provide preliminary evidence to link the induction of this 14-3-3 gene to copper exposure in this alga. Interestingly, the threshold exposure profile may be associated with a decrease in the organism's ability to control copper influx so that it perceives copper as a toxic response.

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“…Pawlik-Skowronska et al 2007). During such maintenance, untreated algae maintained their full viability as evaluated by growth, photosynthesis and metabolism Smith et al 1982).…”

Section: Algal Exposuresmentioning

confidence: 99%

Induction of expression of a 14-3-3 gene in response to copper exposure in the marine alga, Fucus vesiculosus

et al. 2011

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“…PI and cardiolipin (diphosphatidylglycerol) may also be detected (Hofmann and Eichenberger, 1997;Smith et al, 1982). Moreover, earlier studies described an unknown phospholipid-containing phosphoglyceride with two long-chain PUFA and an amino-containing polar group (Eichenberger et al, 1993;Schmid et al, 1994), which was isolated by Eichenberger et al (1995) and described as a phosphatidyl-O-[N-(2-hydroxyethyl)glycine].…”

Section: Brown Seaweedsmentioning

confidence: 99%

Lipids, Fatty Acids, Glycolipids, and Phospholipids

Wielgosz‐Collin

Kendel²,

Couzinet‐Mossion

2016

Seaweed in Health and Disease Prevention

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“…C]glycerol for up to 8 h [13]. After incubation, the calli were rinsed briefly with 100 mm sorbitol and then inactivated by heating in 1.25 mL propan-2-ol for 30min at 70°C and quantitative extraction was carried out as described by Garbus et al [33] and modified for plant tissues [34].…”

Section: Radiolabelling Studies With Culturesmentioning

confidence: 99%

Metabolic control analysis reveals an important role for diacylglycerol acyltransferase in olive but not in oil palm lipid accumulation

et al. 2005

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We applied metabolic control analysis to the Kennedy pathway for triacylglycerol formation in tissue cultures from the important oil crops, olive (Olea europaea L.) and oil palm (Elaeis guineensis Jacq.). When microsomal fractions were incubated at 30 °C rather than 20 °C, there was an increase in triacylglycerol labelling. This increase was accompanied by a build up of diacylglycerol (DAG) radioactivity in olive but not in oil palm, suggesting that the activity of DAG acyltransferase (DAGAT) was becoming limiting in olive. We used 2‐bromooctanoate as a specific inhibitor of DAGAT and showed that the enzyme had a flux control coefficient under the experimental conditions of 0.74 in olive but only 0.12 in oil palm. These data revealed important differences in the regulation of lipid biosynthesis in cultures from different plants and suggest that changes in the endogenous activity of DAGAT is unlikely to affect oil accumulation in oil palm crops.

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Phospholipid metabolism in the brown alga, Fucus serratus

Cited by 48 publications

References 15 publications

Induction of expression of a 14-3-3 gene in response to copper exposure in the marine alga, Fucus vesiculosus

Induction of expression of a 14-3-3 gene in response to copper exposure in the marine alga, Fucus vesiculosus

Lipids, Fatty Acids, Glycolipids, and Phospholipids

Metabolic control analysis reveals an important role for diacylglycerol acyltransferase in olive but not in oil palm lipid accumulation

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