Phospholipid metabolism and membrane dynamics

Alb, James G.; Kearns, Melissa A.; Bankaitis, Vytas A.

doi:10.1016/s0955-0674(96)80093-7

Cited by 9 publications

(9 citation statements)

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“…Since Sac1p is a constituent of both ER and Golgi membranes, this protein may be involved in compartment‐specific signaling events potentially via inositol phospholipids. Generation of transport vesicles is a highly regulated process that also depends on specific changes in the membrane distribution of specific types of phospholipids (Cleves et al ., 1991b; Alb et al ., 1996; Ktistakis et al ., 1996; Chen et al ., 1997). In addition, there is increasing evidence that phosphoinositides such as PtdIns(4)P, PtdIns(3)P and PtdIns(4,5)P 2 have important roles in regulating specific intracellular membrane trafficking events (De Camilli et al ., 1996).…”

Section: Discussionmentioning

confidence: 99%

Sac1p plays a crucial role in microsomal ATP transport, which is distinct from its function in Golgi phospholipid metabolism

et al. 1999

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Analysis of microsomal ATP transport in yeast resulted in the identification of Sac1p as an important factor in efficient ATP uptake into the endoplasmic reticulum (ER) lumen. Yet it remained unclear whether Sac1p is the authentic transporter in this reaction. Sac1p shows no homology to other known solute transporters but displays similarity to the N-terminal non-catalytic domain of a subset of inositol 5Ј-phosphatases. Furthermore, Sac1p was demonstrated to be involved in inositol phospholipid metabolism, an activity whose absence contributes to the bypass Sec14p phenotype in sac1 mutants. We now show that purified recombinant Sac1p can complement ATP transport defects when reconstituted together with sac1Δ microsomal extracts, but is unable to catalyze ATP transport itself. In addition, we demonstrate that sac1Δ strains are defective in ER protein translocation and folding, which is a direct consequence of impaired ATP transport function and not related to the role of Sac1p in Golgi inositol phospholipid metabolism. These data suggest that Sac1p is an important regulator of microsomal ATP transport providing a possible link between inositol phospholipid signaling and ATP-dependent processes in the yeast ER.

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Section: Discussionmentioning

confidence: 99%

Sac1p plays a crucial role in microsomal ATP transport, which is distinct from its function in Golgi phospholipid metabolism

et al. 1999

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“…No higher plant PITPs have yet been described. As fungal and metazoan PITPs are now understood to play critical roles in various secretory and signal‐transducing processes (Alb et al ., 1996; Milligan et al ., 1997), an analysis of higher plant PITPs is warranted.…”

Section: Discussionmentioning

confidence: 99%

“…The Saccharomyces cerevisiae SEC14 gene product (Sec14p) represents the major PITP of yeast, and plays an essential role in protein exit from the yeast Golgi complex (Bankaitis et al ., 1989, 1990). A dissection of how Sec14p translates its PI/PC‐exchange activity to biological function has been driven by the characterization of mutations that relieve cells of the normally essential Sec14p requirement for Golgi function and cell viability (Cleves et al ., 1989, 1991b; Alb et al ., 1996; Fang et al ., 1996). Characterization of these ‘bypass Sec14p’ mutations has generated the proposal that Sec14p maintains the integrity of a critical Golgi diacylglycerol (DAG) pool that is required for Golgi secretory function (McGee et al ., 1994; Kearns et al ., 1997).…”

Section: Introductionmentioning

confidence: 99%

Novel developmentally regulated phosphoinositide binding proteins from soybean whose expression bypasses the requirement for an essential phosphatidylinositol transfer protein in yeast

Kearns

Monks

Fang

et al. 1998

EMBO J

100

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Phosphatidylinositol transfer proteins (PITPs) have been shown to play important roles in regulating a number of signal transduction pathways that couple to vesicle trafficking reactions, phosphoinositide-driven receptor-mediated signaling cascades, and development. While yeast and metazoan PITPs have been analyzed in some detail, plant PITPs remain entirely uncharacterized. We report the identification and characterization of two soybean proteins, Ssh1p and Ssh2p, whose structural genes were recovered on the basis of their abilities to rescue the viability of PITP-deficient Saccharomyces cerevisiae strains. We demonstrate that, while both Ssh1p and Ssh2p share approximately 25% primary sequence identity with yeast PITP, these proteins exhibit biochemical properties that diverge from those of the known PITPs. Ssh1p and Ssh2p represent high-affinity phosphoinositide binding proteins that are distinguished from each other both on the basis of their phospholipid binding specificities and by their substantially non-overlapping patterns of expression in the soybean plant. Finally, we show that Ssh1p is phosphorylated in response to various environmental stress conditions, including hyperosmotic stress. We suggest that Ssh1p may function as one component of a stress response pathway that serves to protect the adult plant from osmotic insult.

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“…In addition, TM_1254 and MTM1, phosphatases known to hydrolyze phosphorylated carbohydrates and phospholipids respectively, were up-regulated in bloom samples. Third, several genes involved in phosphate reallocation, phospholipids-transport and sulphate transport were also up-regulated in bloom samples, such as SEC14, which is involved in the transport of phospholipids from the Golgi complex to other cellular membranes (Bankaitis et al, 1989;Alb et al, 1996). In Arabidopsis, SEC14 was significantly up-regulated by P starvation (Thibaud et al, 2010).…”

Section: Elevated Capacity Of Phosphorus and Nitrogen Uptake And Metamentioning

confidence: 99%

Metatranscriptome analysis reveals environmental and diel regulation of a Heterosigma akashiwo (raphidophyceae) bloom

Lin

et al. 2018

Environmental Microbiology

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Despite numerous laboratory studies on physiologies of harmful algal bloom (HAB) species, physiologies of these algae during a natural bloom are understudied. Here, we investigated a bloom of the raphidophyte Heterosigma akashiwo in the East China Sea in 2014 using metabarcode (18S rDNA) and metatranscriptome sequencing. Based on 18S rDNA analyses, the phytoplankton community shifted from high diversity in the pre-bloom stage to H. akashiwo predominance during the bloom. A sharp decrease in ambient dissolved inorganic phosphate and strong up-regulation of phosphate and dissolved organic phosphorus (DOP) uptake genes, including the rarely documented (ppGpp)ase, in H. akashiwo from pre-bloom to bloom was indicative of rapid phosphorus uptake and efficient utilization of DOP that might be a driver of the H. akashiwo bloom. Furthermore, observed up-regulated expression of mixotrophy-related genes suggests potential contribution of mixotrophy to the bloom. Accelerating photosynthetic carbon fixation was also implied by the up-regulation of carbonic anhydrase genes during the bloom. Notably, we also observed a strong morning-to-afternoon shift in the expression of many genes. Our findings provide insights into metabolic processes likely important for H. akashiwo bloom formation, and suggest the need to consider timing of sampling in field studies on this alga.

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Phospholipid metabolism and membrane dynamics

Cited by 9 publications

References 0 publications

Sac1p plays a crucial role in microsomal ATP transport, which is distinct from its function in Golgi phospholipid metabolism

Sac1p plays a crucial role in microsomal ATP transport, which is distinct from its function in Golgi phospholipid metabolism

Novel developmentally regulated phosphoinositide binding proteins from soybean whose expression bypasses the requirement for an essential phosphatidylinositol transfer protein in yeast

Metatranscriptome analysis reveals environmental and diel regulation of a Heterosigma akashiwo (raphidophyceae) bloom

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