Phospholipid fatty acids analysis of the vertical distribution of microbial communities in eutrophic lake sediments

Abstract

Vertical distribution of microbial communities in a eutrophic lake sediments of Lake Xuanwu was quantified by phospholipid fatty acids analysis and multivariate statistical analysis was employed to interprete the data. Principle component analysis of sediment characteristics parameters, including total nitrogen, total phosphorus, organic matters and pH produced clustering of sampling sites for two distinct groups. These groups corresponded with the two sampling stations and the levels of nutrient enrichment. Total phospholipid fatty acids concentration, which is indicative of microbial biomass, reduced with depth, however, the relative percentage of anaerobic prokaryotes increased. To assess changes of microbial community along depth, phospholipid fatty acids compositions were analyzed by cluster analysis. Distinct clusters were observed in different sampling stations. Canonical correspondence analysis was carried out to infer the relationship between sediment characteristics and microbial communities. Phospholipid fatty acids samples collected at the same sampling site clustered together. Canonical correspondence analysis revealed that the environmental parameter with the greatest bearing on the phospholipid fatty acids profiles was pH. This study proved the successful application of phospholipid fatty acids and multivariate analysis to investigate the relationship between environment factors and microbial community composition.

Distribution of sediment bacterial and archaeal communities in plateau freshwater lakes

“…So far, the diversity and structure of bacterial community in lake sediment have been extensively investigated. Lake sediment bacterial community can be shaped by a variety of environmental factors, such as sediment depth (Nam et al 2008;Shivaji et al 2011;Villaescusa et al 2011), salinity (Dai et al 2013;Jiang et al 2007;Swan et al 2010), phosphorus (Song et al 2012;Zeng et al 2009), organic matter (Bai et al 2012;Zeng et al 2009), pH (Zeng et al 2009;Zhao et al 2011), and pollution (Haller et al 2011). These results were obtained based on the interlake investigations.…”

Microbial community composition in alpine lake sediments from the Hengduan Mountains

“…Thus, the microbial composition of alpine lake sediments is affected. Sediment SWC (Guo et al., ), pH (Zhao et al., ), DO (Yadav, Khardenavis, & Kapley, ), depth (Zhang et al., ), and geographic factors such as altitude (Chen et al., ) can also influence the composition of microbial communities. From the sample–environment relationship (Figure b) we found that the two groups that are geographically close (DK, WD‐2, and ZN; NB‐1 and SL) are distributed far apart in the figure.…”

“…Thus, the microbial composition of alpine lake sediments is affected. Sediment SWC (Guo et al, 2015), pH (Zhao et al, 2011), DO (Yadav, Khardenavis, & Kapley, 2014), depth ,…”

Effects of Hydrostatic Pressure on the Nitrogen Cycle of Sediment

“…The initial detrended correspondence analysis (DCA) results demonstrated that the data exhibited linear rather than unimodal response to the environmental variables (most gradient lengths were 0.224, < 2) [48][49][50], so RDA was performed to explain the data by CANOCO 4.5 (Biometris, Wageningen, Netherlands) [45][46]. Ordination biplots including phospholipid fatty acid composition and environmental variables were used to explain the data.…”