Phospholipid-Derived Fatty Acids and Quinones as Markers for Bacterial Biomass and Community Structure in Marine Sediments

Kunihiro, Tadao; Veuger, Bart; Vasquez‐Cardenas, Diana; Pozzato, Lara; Guitton, M. Le; Moriya, Kazuyoshi; Kuwae, Michinobu; Omori, Koji; Boschker, Henricus T. S.; Oevelen, Dick van

doi:10.1371/journal.pone.0096219

Cited by 17 publications

(11 citation statements)

References 88 publications

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“…4). This is at the high end of contributions from these compounds elsewhere, such as 34 %-45 % in the Arabian Sea and 41 % on the Galicia Bank (Kunihiro et al, 2014). The relatively high proportions of C16 : 1ω7 and C18 : 1ω7 are indicative of the presence of chemosynthetic and specifically sulfide oxidising bacteria (Colaco et al, 2007).…”

Section: Bacterial Uptake and Plfa Suitementioning

confidence: 97%

Benthic carbon fixation and cycling in diffuse hydrothermal and background sediments in the Bransfield Strait, Antarctica

et al. 2020

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Abstract. Sedimented hydrothermal vents are likely to be widespread compared to hard substrate hot vents. They host chemosynthetic microbial communities which fix inorganic carbon (C) at the seafloor, as well as a wide range of macroinfauna, including vent-obligate and background non-vent taxa. There are no previous direct observations of carbon cycling at a sedimented hydrothermal vent. We conducted 13C isotope tracing experiments at three sedimented sites in the Bransfield Strait, Antarctica, which showed different degrees of hydrothermalism. Two experimental treatments were applied, with 13C added as either algal detritus (photosynthetic C), or as bicarbonate (substrate for benthic C fixation). Algal 13C was taken up by both bacteria and metazoan macrofaunal, but its dominant fate was respiration, as observed at deeper and more food-limited sites elsewhere. Rates of 13C uptake and respiration suggested that the diffuse hydrothermal site was not the hot spot of benthic C cycling that we hypothesised it would be. Fixation of inorganic C into bacterial biomass was observed at all sites, and was measurable at two out of three sites. At all sites, newly fixed C was transferred to metazoan macrofauna. Fixation rates were relatively low compared with similar experiments elsewhere; thus, C fixed at the seafloor was a minor C source for the benthic ecosystem. However, as the greatest amount of benthic C fixation occurred at the “Off Vent” (non-hydrothermal) site (0.077±0.034 mg C m−2 fixed during 60 h), we suggest that benthic fixation of inorganic C is more widespread than previously thought, and warrants further study.

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Section: Bacterial Uptake and Plfa Suitementioning

confidence: 97%

Benthic carbon fixation and cycling in diffuse hydrothermal and background sediments in the Bransfield Strait, Antarctica

et al. 2020

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“…), substrate‐induced respiration (Johansen and Olsson ) and respiratory quinone concentrations (Kunihiro et al . ). However, Frostegård et al .…”

Section: Applications and Interpretations Of Plfa Datamentioning

confidence: 97%

Phospholipid fatty acid profiling of microbial communities-a review of interpretations and recent applications

2015

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Keywords fungal to bacterial ratio, microbial activity, microbial biomass, microbial community structure, phospholipid fatty acid, physiological stress ratio. Summary Profiling of microbial communities in environmental samples often utilizes phospholipid fatty acid (PLFA) analysis. This method has been used for more than 35 years and is still popular as a means to characterize microbial communities in a diverse range of environmental matrices. This review examines the various recent applications of PLFA analysis in environmental studies with specific reference to the interpretation of the PLFA results. It is evident that interpretations of PLFA results do not always correlate between different investigations. These discrepancies in interpretation and their subsequent applications to environmental studies are discussed. However, in spite of limitations to the manner in which PLFA data are applied, the approach remains one with great potential for improving our understanding of the relationship between microbial populations and the environment. This review highlights the caveats and provides suggestions towards the practicable application of PLFA data interpretation.

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“…Therefore, quinone distributions in the environment complement the quantitative information encoded in intact polar lipid (IPL) abundances by functional constraints related to microbial metabolisms and redox conditions. Combined quinone and membrane lipid distributions obtained by separate analyses have previously been employed for studying bacterial diversity and redox state in microbial mats (Villanueva et al ., 2007; 2010) and sediments (Kunihiro et al ., ), while archaeal quinones have so far not been studied in the environment.…”

Section: Introductionmentioning

confidence: 99%

Respiratory quinones in Archaea: phylogenetic distribution and application as biomarkers in the marine environment

Elling

Becker

Könneke

et al. 2015

Environmental Microbiology

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The distribution of respiratory quinone electron carriers among cultivated organisms provides clues on both the taxonomy of their producers and the redox processes these are mediating. Our study of the quinone inventories of 25 archaeal species belonging to the phyla Eury-, Cren- and Thaumarchaeota facilitates their use as chemotaxonomic markers for ecologically important archaeal clades. Saturated and monounsaturated menaquinones with six isoprenoid units forming the alkyl chain may serve as chemotaxonomic markers for Thaumarchaeota. Other diagnostic biomarkers are thiophene-bearing quinones for Sulfolobales and methanophenazines as functional quinone analogues of the Methanosarcinales. The ubiquity of saturated menaquinones in the Archaea in comparison to Bacteria suggests that these compounds may represent an ancestral and diagnostic feature of the Archaea. Overlap between quinone compositions of distinct thermophilic and halophilic archaea and bacteria may indicate lateral gene transfer. The biomarker potential of thaumarchaeal quinones was exemplarily demonstrated on a water column profile of the Black Sea. Both, thaumarchaeal quinones and membrane lipids showed similar distributions with maxima at the chemocline. Quinone distributions indicate that Thaumarchaeota dominate respiratory activity at a narrow interval in the chemocline, while they contribute only 9% to the microbial biomass at this depth, as determined by membrane lipid analysis.

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Phospholipid-Derived Fatty Acids and Quinones as Markers for Bacterial Biomass and Community Structure in Marine Sediments

Cited by 17 publications

References 88 publications

Benthic carbon fixation and cycling in diffuse hydrothermal and background sediments in the Bransfield Strait, Antarctica

Benthic carbon fixation and cycling in diffuse hydrothermal and background sediments in the Bransfield Strait, Antarctica

Phospholipid fatty acid profiling of microbial communities-a review of interpretations and recent applications

Respiratory quinones in Archaea: phylogenetic distribution and application as biomarkers in the marine environment

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