Phospholipid Depletion Techniques in LC‐MS Bioanalysis

Brown, Stacy D.; Carmical, Jennifer

doi:10.1002/9781119274315.ch4

Cited by 1 publication

(1 citation statement)

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“…Twenty cohort study analyses examined the relationship between mass spectrometry (MS), which uses strongly sensitive identification techniques, and the ionization of sample components using multiple methods (Jalal et al 2018). It was found that the separation of resulting ions in a vacuum was according to the mass charge ratios as well as the intensity of each ion (Brown and Carmical 2019).…”

Section: Introductionmentioning

confidence: 99%

Rapid detection and identification of dioscorine compounds in Dioscorea hispida tuber plants by LC-ESI-MS

Kamaruddin¹,

Sapuan

Zuhri

et al. 2020

BioRes

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Studies have revealed that Dioscorea hispida tubers contain a poisonous substance called alkaloid dioscorine. The method for removing alkaloid dioscorine in Dioscorea hispida is explored in this research through a soaking process. The tubers were peeled, washed, sliced, and soaked for 5 days in either 1.0 M sodium chloride (NaCl) or distilled water. The aim of this study was to firstly identify the amount of toxic dioscorine remaining after soaking for 5 days, and then determine the best solution for removing dioscorine compounds in the tubers that were obtained from a tropical area in Peninsular Malaysia. The liquid chromatography electrospray ionization mass spectrometric (LC-ESI-MS) systems were used to identify the presence of alkaloid dioscorine compounds within Dioscorea hispida tubers. The analysis showed that no dioscorine compounds were present in day 5 for samples soaked in the NaCl solution. However, the relative abundance in the distilled water solution at day 5 was 281000, indicating a 95% decrease of the relative abundance value of the dioscorine compounds. This work aimed to determine the minimum days needed to remove the poisonous element before Dioscorea hispida tubers could be used for food consumption or for any other applications.

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Section: Introductionmentioning

confidence: 99%