Phospholipid Containing Choline Histochemistry of Mouse Uterine Epithelia during Preimplantation Stage

Paiva, Cintia S. de; Alberto-Rincon, M. C.; Paiva, S. M. de; Bechara, I. J.; Yamada, Áureo T.

doi:10.3109/10520299709082239

Cited by 2 publications

(5 citation statements)

References 32 publications

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“…Similar to our results, LDs were detected mainly in LE but not in other uterine compartments of non-pregnant sheep [23,24]. In addition, LDs were detected in luminal and glandular epithelium, and stroma in pregnant mice [25,26], in uterine epithelium and/or stroma of non-pregnant, pregnant or ovariectomized rats [29][30][31], and in human fetal membranes and placenta at term [34,35]. Accumulation of LDs only within selected compartments of uteri and placenta indicates the specific role of LDs in endometrium.…”

Section: Discussionsupporting

confidence: 90%

“…The specific role of LDs in uterine function has not been studied in detail, however, it has been indicated that lipid signaling is involved in preparation of the uterus for implantation [93], and that expression of LDs in endometrium is associated with prostaglandin signaling [26] and energy storage [31]. Therefore, we postulate that LDs may be involved in PG production/function/metabolism/signaling, steroidogenesis/steroid signaling, and some other processes that require additional energy sources.…”

Section: Revised 12mentioning

confidence: 95%

“…Lipid droplets are present in the uterine compartments of sheep [23,24], mice [25][26][27][28], rats [29][30][31], cows [32,33] and in human placenta at term [34,35]. The uterine expression of LDs depends on the stage of the estrous cycle or pregnancy in several species [23,26,[31][32][33].…”

Section: Revisedmentioning

confidence: 99%

“…Furthermore, it has been postulated that progesterone (P4), estradiol-17β (E2), and prostaglandins (PG) are involved in the regulation of LDs in the uterus and placenta [23,26,28,31,36,37].…”

Section: Revisedmentioning

confidence: 99%

“…High expression of LDs at the late-luteal phase may be associated with increased secretion of PG by endometrium in non-pregnant animals when LDs may serve as the source of precursors for PG biosynthesis [26]. Based on limited data, we hypothesize that endometrial LD expression is regulated by steroid hormones and other factors controlling reproductive cyclicity, and regulation of LD expression within the endometrium may be species specific.…”

mentioning

confidence: 98%

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Lipid droplets in the ovine uterus during the estrous cycle: Effects of nutrition, arginine, and FSH

et al. 2017

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The aim of this study was to evaluate lipid droplet (LD) expression in uteri of FSH-treated or nontreated sheep administered with arginine (Arg) or vehicle (saline, Sal) and fed a control (C), excess (overfed, O) or restricted (underfed, U) diet. In experiment 1, ewes from each diet were randomly assigned to Arg or Sal treatments administered three times daily starting on Day 0 of the first estrous cycle until blood sample and uterine tissue collection at the early- or mid-luteal phase of the second estrous cycle or the late-luteal phase of the first estrous cycle. In experiment 2, ewes were injected twice daily with FSH on Days 13 to 15 of the first estrous cycle, and blood samples and uterine tissue were collected at the early- and mid-luteal phases of the second estrous cycle. Cryopreserved in optimum cutting temperature (OCT) compound, cross-sections of uterine horn were stained with boron-dipyrromethene (BODIPY; marker of LDs) followed by 4',6-diamidino-2-phenylindole (DAPI) staining and image analysis to determine the proportion (%) of area occupied by LD in luminal epithelium (LE) and endometrial glands (EGs). Control ewes maintained, O ewes gained, and U ewes lost body weight during the experiments. Serum progesterone concentration was not affected by nutritional plane or Arg treatment and was 5.5-fold greater in FSH- than Sal-treated ewes. LDs were detected in LE and superficial EG (close to LE) but not deep EG, or other uterine compartments, and area occupied by LD was greater in LE than in EG. In experiment 1, in LE and EG, area occupied by LDs was greater in C than in O or U; greater in Arg than in Sal; and greater at the late-, less at mid-, and least at early-luteal phases. In experiment 2, in LE and EG, area occupied by LDs was greater at mid- than in early-luteal phase. Comparison of data from FSH-treated and nontreated ewes (e.g., experiment 1 vs. experiment 2) demonstrated that FSH increased the area occupied by LD in LE and EG regardless of diet. Interactions between FSH treatment, stage of the estrous cycle, and plane of nutrition demonstrated that FSH increased the area occupied by LD in LE and EG at the mid-luteal phase in O and U. Thus, LDs are differentially distributed in uterine compartments, and area occupied by LD in endometrium is affected by nutritional plane, Arg or FSH, and stage of the estrous cycle. Such changes in dynamics of LD in the endometrium during the estrous cycle indicate their specific role in uterine functions.

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