Phospholipid bilayer formation on hydroxyapatite sol–gel synthesized films

Nellis, Barbara A.; Satcher, Joe H.; Risbud, Subhash H.

doi:10.1016/j.colsurfb.2010.10.016

Cited by 9 publications

(8 citation statements)

References 30 publications

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“…Similar diffusion coefficients have been reported for phospholipid bilayers deposited on different materials such as mica and xerogel films composed of iron oxide, phloroglucinol-formaldehyde or silica. 64,[67][68][69] In these works decrease of the lipid mobility was attributed to the interaction occurring between the bilayer and the material surface. These results support the hypothesis that electrostatic interactions are taking place between the negatively charged silanol groups located in the pore wall of the host matrix and the zwitterionic phospholipids in the outer membrane leaflet.…”

Section: Effect Of Sol-gel Encapsulation On the Lipid Domain Coexistencementioning

confidence: 99%

Immobilization and characterization of giant unilamellar vesicles (GUVs) within porous silica glasses

Esquembre

Pinto²,

Poveda

et al. 2012

Soft Matter

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Immobilization of cells or artificial liposomes has interesting applications in protein biology, membrane biophysics, biomedicine, biosensor technology and new materials development. In this work for the first time we have entrapped giant unilamellar vesicles (GUVs) in silica glasses prepared by the sol-gel process. Results show that GUVs are successfully confined in the porous matrix retaining their structural integrity for at least fifteen days, allowing single-vesicle studies to be performed. Using different fluorescence microscopy approaches, we have studied the effect of the encapsulation on membrane properties, such as their size and shape, hydration degree, domain coexistence and lipid lateral mobility. Results reveal that these properties are altered to a more or less degree after immobilization, but most of vesicles are affected in a similar fashion and no different populations are distinguished. Such effects are attributed to the increase in lateral packing induced by changes in the hydrostatic and/or osmotic pressure occurring during the sol-gel process, as well as to the establishment of interactions between the polar head of the phospholipids and the negatively charged silica surface of the porous matrix.

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Section: Effect Of Sol-gel Encapsulation On the Lipid Domain Coexistencementioning

confidence: 99%

Immobilization and characterization of giant unilamellar vesicles (GUVs) within porous silica glasses

Esquembre

Pinto²,

Poveda

et al. 2012

Soft Matter

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show abstract

“…Many researches have been directed to coating the titanium surface by a bioactive layer namely hydroxyapatite to improve the tissue osteointegration [4][5][6]. Hydroxyapatite (HA, Ca 10 (PO 4 ) 6 (OH) 2 ), the bioactive ceramic material, has been utilized in dentistry and medicine in recent years due to its excellent biocompatibility and osteoconduction [7][8][9][10]. It is a major inorganic component of natural bone and can accelerate the bone growth into the titanium implants [11,12].…”

Section: Introductionmentioning

confidence: 99%

An innovative technique to simply fabricate ZrO2–HA–TiO2 nanostructured layers

Samanipour

Bayati

Golestani‐Fard

et al. 2011

Colloids and Surfaces B: Biointerfaces

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“…Also, nano-HA can be conjugated with organic polymers to form an organic–inorganic composite . Many techniques have been used to create composites including electrospinning, co-precipitation, an alternate soaking process, and sol–gel casting . However, these methods have the limitations of not being able to form nanosized HA deposits in the composite, and some of the HA are not uniformly dispersed in the composite.…”

Section: Introductionmentioning

confidence: 99%

“…19 Many techniques have been used to create composites including electrospinning, 20 co-precipitation, 21 an alternate soaking process, 22 and sol−gel casting. 23 However, these methods have the limitations of not being able to form nanosized HA deposits in the composite, and some of the HA are not uniformly dispersed in the composite. Thus, new strategies are required to optimize the preparation of ideal organic−inorganic composite scaffolds.…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensionally N-Doped Graphene–Hydroxyapatite/Agarose as an Osteoinductive Scaffold for Enhancing Bone Regeneration

Luo

Zhang

Machuki

et al. 2018

ACS Appl. Bio Mater.

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Composite biomaterials with hierarchical structures have emerged as new approaches for bone-tissue engineering. In this study, a biomimetic, osteoconductive tricomposite scaffold made of N-doped graphene–hydroxyapatite (NG–HA) hybrids blended with an agarose (AG) matrix was prepared via a facile hydrothermal/cross-linking/freeze-drying method. The structure and composition of AG/NG–HA were examined by scanning electron microscopy, X-ray photoelectron spectroscopy, X-ray diffraction, Fourier transform infrared, Raman spectroscopy, and thermogravimetric analysis. The as-prepared scaffolds showed hierarchical pore architecture and an organic–inorganic composition, which simulated the composition and structure of natural bone tissue. The effect of AG/NG–HA on bone mesenchymal stem cells (MSCs) osteoblast proliferation, differentiation, and mineralization was tested in vitro. The expression of osteogenic-related genes was determined by real-time polymerase chain reaction. Our results showed that the introduction of N-graphene into the hybrid scaffold significantly improved its mechanical properties, an effect that promoted the proliferation and viability of MSCs. Moreover, the scaffolds triggered selective differentiation of MSCs to osteogenic lineage while conferring good cell adhesion, enhanced alkaline phosphatase activity, and mineralization. A distal femoral condyle critical size defect in rabbits was used as a platform to confirm the effect of AG/NG–HA on bone regeneration in vivo. Our experiments show that the AG/NG–HA hybrid scaffolds provided a favorable environment for new bone formation. The results presented in this study suggest that the AG/NG–HA hybrid scaffolds have potential in bone-tissue regeneration engineering.

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Phospholipid bilayer formation on hydroxyapatite sol–gel synthesized films

Cited by 9 publications

References 30 publications

Immobilization and characterization of giant unilamellar vesicles (GUVs) within porous silica glasses

Immobilization and characterization of giant unilamellar vesicles (GUVs) within porous silica glasses

An innovative technique to simply fabricate ZrO2–HA–TiO2 nanostructured layers

Three-Dimensionally N-Doped Graphene–Hydroxyapatite/Agarose as an Osteoinductive Scaffold for Enhancing Bone Regeneration

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