Phospholipid and Fatty Acid Characteristics of Erythrocytes in Some Cases of Anaemia

Gier, J. De; Deenen, L.L.M. Van; Verloop, M. C.; Gastel, C. van

doi:10.1111/j.1365-2141.1964.tb00699.x

Cited by 53 publications

(8 citation statements)

References 21 publications

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“…Previous studies have indicated that microspherocytes disappear after splenectomy [l], that total lipid content is decreased by 1520% in red cells of HS patients [21,23,24] who have spleens and are hemolyzing, and that the total lipid content of HS red cells after splenectomy is similar to that found in red cells from normal subjects with spleens [21]. It has also been observed that the relative proportions of cholesterol, total phospholipids, the various phospholipid fractions [25,26], and fatty acid composition [25,27] are normal. Based on the results published previously, the decrease of surface area in microspherocytosis appears to be related to the decreased membrane lipids.…”

Section: Discussionmentioning

confidence: 76%

Disappearance of microspherocytes in peripheral circulation and normalization of decreased lipids in plasma and in red cells of patients with hereditary spherocytosis after splenectomy

Sugihara

Yawata

1984

American J Hematol

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A possible mechanism of the formation of microspherocytosis in unsplenectomized patients with hereditary spherocytosis (HS) was investigated in relation to lipid metabolism in plasma and in red cells of these patients. Plasma lipids (total cholesterol, free cholesterol, high density lipoprotein cholesterol, free fatty acids, and phospholipids) were markedly reduced in unsplenectomized HS patients with microspherocytosis. Red cell membrane lipids (free cholesterol and phospholipids such as phosphatidyl ethanolamine, sphingomyelin, phosphatidyl choline, and lysophosphatidyl choline) were also decreased in these unsplenectomized HS patients. After splenectomy, microspherocytosis disappeared concomitant to substantial normalization of plasma and red cell membrane lipids. These observations suggest that plasma lipid decrement in the unsplenectomized HS patients is at least one of the causative factors in pathogenesis of the formation of microspherocytosis. So-called "splenic conditioning" may not imply a physical "loss" of membrane by fragmentation or pitting of the once-formed HS red cells in the peripheral circulation. Instead, the decreased plasma lipids in the presence of the spleen may affect the de novo synthesis of red cell membrane lipids, resulting in the formation of microspherocytosis.

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Section: Discussionmentioning

confidence: 76%

Disappearance of microspherocytes in peripheral circulation and normalization of decreased lipids in plasma and in red cells of patients with hereditary spherocytosis after splenectomy

Sugihara

Yawata

1984

American J Hematol

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“…Following spleiiectomy, red cell lipids have been found to be normal. The red-cell content of cholesterol and the composition of phospholipids arc not affected in HS (de Gier et al, 1964;Phillips & Roome, 1962). It was therefore coiicluded (Reed & Swishcr, 1966;Cooper & Jandl, 1969) that factors other than a change in overall lipid content or lipid composition are responsible for the observed increase in osmotic fragility and for the decrese in surface area of spherocytic red cells.…”

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confidence: 98%

Linolenoyl Sorbitol and the Fragility of Hereditary Spherocytes

et al. 1973

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“….. (235,236). An early study of age-related fatty acid composition changes indicated that in general the ~ontent of unsaturated fatty acids increase while that of the saturateq fatty acids decre~sel w~th 411…”

Section: Fatty Acid Composition Cbangesmentioning

confidence: 99%

Some Characteristics of the Exchange of Lecithin between Rabbit Erythrocytes and Serum

Smith¹,

Rubinstein²

1974

Can. J. Biochem.

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The exchange of lecithin between rabbit erythrocytes and serum has been investigated. The erythrocyte lecithin was labelled in vivo by administration of Na332PO4, which resulted in the acquisition of [32PJlecithin by the cells primarily by exchange, and in vitro by esterification with [3H]palmitate, [14C]linoleate, or [14C]linolenate. The rate of exchange of erythrocyte [32PJ-phospholipids was independent of specific serum lipoprotein fractions, but was related to the serum phospholipid concentration. Analysis of the erythrocyte inward and outward exchange rates of [32P]lecithin revealed that only 55% of the cellular lecithin pool takes part in the exchange. A similar conclusion can be reached by comparison of the fractional exchange rates of the acyl- and 32P-labelled erythrocyte lecithin, or by comparison of the ratio of acyl to 32P radioactivity of the erythrocyte lecithin with the ratio found in the serum after exchange. These data indicated that the proportion of the acyl-labelled erythrocyte lecithin pool (± S.E.M.) capable of exchange was 53 ± 5%, 64 ± 12%, and 95 ± 6%, when esterified with [14C]linoleate, [3H]palmitate, and [14C]linolenate, respectively. The differences in the proportion of the acyl-labelled erythrocyte lecithin pool undergoing exchange could not be attributed to the preferential exchange of a particular molecular species of lecithin, although the tetraenoic lecithin did exchange to a relatively greater degree. This suggests that cellular lecithin formed by esterification of linolenate is found only in the portion of the pool available for exchange, whereas that formed by esterification of linoleate and probably palmitate is distributed throughout the entire lecithin pool. The fractional exchange rate of [32P]lecithin of isolated erythrocyte membranes was 3 times that of the lecithin of intact cells. However, the difference in fractional exchange rates between [3H]palmityl-lecithin or [14C]linoleyl-lecithin and [32P]lecithin is reversed in the membranes. These observations suggest that a portion of the total cellular lecithin pool becomes available for exchange only upon disruption of the erythrocyte, and may be located in a relatively inaccessible portion of the membrane such as the inner surface.

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Phospholipid and Fatty Acid Characteristics of Erythrocytes in Some Cases of Anaemia

Cited by 53 publications

References 21 publications

Disappearance of microspherocytes in peripheral circulation and normalization of decreased lipids in plasma and in red cells of patients with hereditary spherocytosis after splenectomy

Disappearance of microspherocytes in peripheral circulation and normalization of decreased lipids in plasma and in red cells of patients with hereditary spherocytosis after splenectomy

Linolenoyl Sorbitol and the Fragility of Hereditary Spherocytes

Some Characteristics of the Exchange of Lecithin between Rabbit Erythrocytes and Serum

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