Phospholipase stimulates lipogenesis in SZ95 sebocytes

Abstract

Introduction: With progressing ageing human sebocytes reduce lipid production. However, the influence of certain aging mechanisms on sebaceous lipid synthesis as well as ways to influence the latter is not fully identified. Certain lipids act as ligands of nuclear receptors such as PPAR. Phospholipase (PLA2) catalyzes the hydrolysis of the sn‐2 fatty acyl bond of phospholipids to yield free fatty acid and lysophospholipid. It has been hypothesized that PPAR may be activated by hydrolysis products of phospholipids and also by eicosanoids obtained through PLA2 activity. Materials and Methods: A method to quantify sebaceous lipid synthesis of SZ95 sebocytes in vitro was established and the cells were treated by snake venom Bothrops moojeni gel filtration fractions (Botmo GF). Botmo GF fractions were further purified by RP‐HPLC, and a fraction with PLA2 activity (Botmo GF11‐117) and a fraction without enzymatic activity (Botmo GF11‐101) were identified and additionally tested. Results: Botmo GF fractions increased lipogenesis in SZ95 sebocytes without inducing apparent toxic or apoptotic effects. Botmo GF11‐101 (1 μg/ml) enhanced neutral lipid synthesis by up to 170% and polar lipid synthesis by up to 120%. The enzymatically active PLA2 Botmo GF11‐117 (1 μg/ml) increased synthesis of neutral lipids by up to 200%, and polar lipids by up to 120% compared to untreated SZ95 sebocytes. Conclusion: PLA2 activation or suppression could be important for human sebaceous lipogenesis. PLA2 modifiers may be attractive for skin lipid research and pharmacological/cosmetic products.

PPARγ-Mediated and Arachidonic Acid–Dependent Signaling Is Involved in Differentiation and Lipid Production of Human Sebocytes

“…Therefore they represent the source for AA production. PLA2 was detected in various cells of the skin and also in SZ95 sebocytes (Schagen et al, 2008) and treatment with active PLA2 increased neutral and polar lipid production of sebocytes (Schagen et al, 2008). It seems possible, that PLA2 is one regulator of endogenous AA production in sebocytes, and it also determines the level of PPARg activation in these cells, at least in part.…”

Lipid regulation in SZ95 sebocytes by active and inactive phospholipases A2fromBothrops moojenivenom

“…Surprisingly, stimulation of lipid synthesis was independent from PLA 2 enzymatic activity in Botmo GF 11 subfractions. 26 There may be different mechanisms which can cause lipid stimulation in sebocytes after Botmo GF 11-101 and Botmo GF 11-117 treatment. Both mentioned pathways, arachidonic acid and lysophosphatidylcholine, may influence the fatty acid production.…”