Phospholipase Dα6 and phosphatidic acid regulate gibberellin signaling in rice

Cao, Huasheng; Gong, Renmin; Yuan, Shuo; Su, Yu‐Nung; Lv, Weixin; Zhou, Yanmin; Zhang, Qingqing; Deng, Xianjun; Tong, Pan; Liang, Shihu; Wang, Xuemin; Hong, Yueyun

doi:10.15252/embr.202051871

Cited by 10 publications

(9 citation statements)

References 45 publications

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“…For At AHL4, PA-mediated tethering to the nuclear membrane was excluded as a mechanism of PA action, suggesting a hypothetical possible association of PA with At AHL4 beyond membranes [ 3 ]. PA also binds to the gibberellin receptor Os GID1, promotes its nuclear localization, and mediates the gibberellin response in rice [ 36 ].…”

Section: Binding Of Phosphatidic Acid To Target Proteinsmentioning

confidence: 99%

“…In rice protoplasts, gibberellin induces OsPLDα6 translocation from cytosol to nuclei. PA formed by OsPLDα6 activity positively mediates gibberellin signaling via PA binding to gibberellin receptor OsGID1, promotion of its nuclear localization and degradation of the DELLA protein SLENDER RICE1, a negative regulator of the hormone signaling [ 36 ]. In contrast, the level of suppressor protein SLENDER RICE1 was decreased in npc6 rice mutants treated with gibberellin, but drastically increased in OsNPC6 over-expressing plants [ 122 ].…”

Section: Role Of Pa As a Lipid Second Messenger Of Signal Transductio...mentioning

confidence: 99%

“…The level of PA in Arabidopsis seedlings detected by GC was found to constitute several mol% [ 35 ]. In rice leaves, the level of PA analyzed by mass spectrometry was observed to be in the range of 2.5 to 5 nmol/mg [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

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Phosphatidic Acid in Plant Hormonal Signaling: From Target Proteins to Membrane Conformations

Kolesnikov

Kretynin

Bukhonska

et al. 2022

IJMS

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Cells sense a variety of extracellular signals balancing their metabolism and physiology according to changing growth conditions. Plasma membranes are the outermost informational barriers that render cells sensitive to regulatory inputs. Membranes are composed of different types of lipids that play not only structural but also informational roles. Hormones and other regulators are sensed by specific receptors leading to the activation of lipid metabolizing enzymes. These enzymes generate lipid second messengers. Among them, phosphatidic acid (PA) is a well-known intracellular messenger that regulates various cellular processes. This lipid affects the functional properties of cell membranes and binds to specific target proteins leading to either genomic (affecting transcriptome) or non-genomic responses. The subsequent biochemical, cellular and physiological reactions regulate plant growth, development and stress tolerance. In the present review, we focus on primary (genome-independent) signaling events triggered by rapid PA accumulation in plant cells and describe the functional role of PA in mediating response to hormones and hormone-like regulators. The contributions of individual lipid signaling enzymes to the formation of PA by specific stimuli are also discussed. We provide an overview of the current state of knowledge and future perspectives needed to decipher the mode of action of PA in the regulation of cell functions.

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Section: Binding Of Phosphatidic Acid To Target Proteinsmentioning

confidence: 99%

Section: Role Of Pa As a Lipid Second Messenger Of Signal Transductio...mentioning

confidence: 99%

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Phosphatidic Acid in Plant Hormonal Signaling: From Target Proteins to Membrane Conformations

Kolesnikov

Kretynin

Bukhonska

et al. 2022

IJMS

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“…In Arabidopsis, PA mediates the tethering of ABI1 (a negative regulator of ABA) to the plasma membrane to prevent it from reaching its downstream targets in the nucleus [ 29 ]. PA could bind to GA receptor OsGID1 to promote its nuclear localization for gibberellin response in rice [ 30 ]. PA is also involved in the process of ETH signaling by binding to CTRI, the negative regulator in the ethylene signaling pathway, and inhibiting its activity.…”

Section: Introductionmentioning

confidence: 99%

Integrated Lipidomic and Transcriptomic Analysis Reveals Phospholipid Changes in Somatic Embryos of Picea asperata in Response to Partial Desiccation

Ling

Xia

Jiwen

et al. 2022

IJMS

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Partial desiccation treatment (PDT) is an effective technology for promoting the germination and conversion of conifer somatic embryos (SEs). PDT, as a drought stress, induces intensive physiological responses in phospholipid metabolism, which are not well understood in the conifer SEs. Here, we integrated lipidomics, transcriptomics and proteomics analyses to reveal the molecular basis of lipid remodeling under PDT in Picea asperata SEs. Among the 82 lipid molecular species determined by mass spectrometry, phosphatidic acid (PA) had a significant effect after PDT and was the most critical lipid in the response to PDT. The transcriptomics results showed that multiple transcripts in the glycerolipid and glycerophospholipid metabolism pathways were differentially expressed, and these included five PLDα1 transcripts that catalyze the conversion of phosphatidylcholine (PC) to PA. Furthermore, the enzyme activity of this phospholipase D (PLD) was significantly enhanced in response to PDT, and PDT also significantly increased the protein level of PLDα1 (MA_10436582g0020). In addition, PA is a key factor in gibberellin, abscisic acid and ethylene signal transduction. One GDI1, one DELLA, three ABI1s, two SnRK2s, one CTR and 12 ERFs showed significantly differential expression between SEs before and after PDT in this study. Our data suggest that the observed increases in the PA contents might result from the activation of PLDα by PDT. PA not only affects the physical and chemical properties of the cell membrane but also participates in plant hormone signal transduction. Our work provides novel insight into the molecular mechanism through which PDT promotes the germination of SEs of coniferous tree species and fills the gap in the understanding of the mechanism of somatic embryo lipid remodeling in response to PDT.

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“…A small change in PA level may result in a substantial effect due to its function in the upstream of signaling cascades [ 20 ]. Phosphatidic acid with various species of acyl group in specific membrane region has been shown to bind to various effectors involved in diverse cellular processes in plants [ 19 , 20 , 21 ]. In plants, PA can be generated through three major routes [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

The Examination of the Role of Rice Lysophosphatidic Acid Acyltransferase 2 in Response to Salt and Drought Stresses

Shaikh

Alfatih

Jia

et al. 2022

IJMS

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Phosphatidic acid (PA) is an important signal molecule in various biological processes including osmotic stress. Lysophosphatidic acid acyltransferase (LPAT) acylates the sn-2 position of the glycerol backbone of lysophosphatidic acid (LPA) to produce PA. The role of LPAT2 and its PA in osmotic stress response remains elusive in plants. Here we showed that LPAT2-derived PA is important for salt and drought stress tolerance in rice. Rice LPAT2 was localized to the endoplasmic reticulum (ER) to catalyze the PA synthesis. The LPAT2 transcript was induced by osmotic stress such as high salinity and water deficit. To reveal its role in osmotic stress response, an LPAT2 knockdown mutant, designated lpat2, was isolated from rice, which contained a reduced PA level relative to wild type (WT) plants under salt stress and water deficit. The lpat2 mutant was more susceptible to osmotic stress and less sensitive to abscisic acid (ABA) than that of WT, which was recovered by either PA supplementation or genetic LPAT2 complementation. Moreover, suppressed LPAT2 also led to a large number of differentially expressed genes (DEGs) involved in diverse processes, particularly, in ABA response, kinase signaling, and ion homeostasis in response to salt stress. Together, LPAT2-produced PA plays a positive role in osmotic tolerance through mediating ABA response, which leads to transcriptional alteration of genes related to ABA response, protein kinase signaling, and ion homeostasis.

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Phospholipase Dα6 and phosphatidic acid regulate gibberellin signaling in rice

Cited by 10 publications

References 45 publications

Phosphatidic Acid in Plant Hormonal Signaling: From Target Proteins to Membrane Conformations

Phosphatidic Acid in Plant Hormonal Signaling: From Target Proteins to Membrane Conformations

Integrated Lipidomic and Transcriptomic Analysis Reveals Phospholipid Changes in Somatic Embryos of Picea asperata in Response to Partial Desiccation

The Examination of the Role of Rice Lysophosphatidic Acid Acyltransferase 2 in Response to Salt and Drought Stresses

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