Phospholipase D inhibitors reduce human prostate cancer cell proliferation and colony formation

Noble, Amanda; Maitland, Norman J.; Berney, Daniel M.; Rumsby, Martin G.

doi:10.1038/bjc.2017.391

Cited by 41 publications

(49 citation statements)

References 73 publications

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“…PLD1 protein expression is also higher in proliferating benign prostate hyperplasia tissue compared with normal or PCa tissue. 43 PLD activity appears not to be elevated in PCa tissue compared with normal tissue, unlike findings with other cancers (see above). PLD1 protein expression is, however, significantly higher in Gleason 7 PCa tissue compared with tissue scored Gleason 9.…”

Section: Introductionmentioning

confidence: 53%

“…The prostate epithelial cell lines used, with their growth media requirement, diagnosis and origin were as described in Noble et al 43 The benign hyperplasia (BPH-1) cell line 45 was cultured in RPMI medium + 5% foetal calf serum.…”

Section: Methodsmentioning

confidence: 99%

“…We have reported that PLD1 protein is preferentially expressed in basal rather than luminal prostate epithelial cell lines and in basal rather than luminal layer cells in normal prostate tissue in situ. 43 In PCa where basal cells gradually become depleted, 44 PLD1 protein expression is detected in the expanding population of luminal cells. PLD1 protein expression is also higher in proliferating benign prostate hyperplasia tissue compared with normal or PCa tissue.…”

Section: Introductionmentioning

confidence: 99%

“…PLD1 protein expression is, however, significantly higher in Gleason 7 PCa tissue compared with tissue scored Gleason 9. 43 In this report we have investigated expression and inhibition of PLD2 in PCa using patient-derived PCa cells, prostate cell lines and tissue microarrays.…”

Section: Introductionmentioning

confidence: 99%

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Phospholipase D2 in prostate cancer: protein expression changes with Gleason score

et al. 2019

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Background Phospholipases D1 and D2 (PLD1/2) are implicated in tumorigenesis through their generation of the signalling lipid phosphatidic acid and its downstream effects. Inhibition of PLD1 blocks prostate cell growth and colony formation. Here a role for PLD2 in prostate cancer (PCa), the major cancer of men in the western world, is examined. Methods PLD2 expression was analysed by immunohistochemistry and western blotting. The effects of PLD2 inhibition on PCa cell viability and cell motility were measured using MTS, colony forming and wound-healing assays. Results PLD2 protein is expressed about equally in luminal and basal prostate epithelial cells. In cells from different Gleason-scored PCa tissue PLD2 protein expression is generally higher than in non-tumorigenic cells and increases in PCa tissue scored Gleason 6–8. PLD2 protein is detected in the cytosol and nucleus and had a punctate appearance. In BPH tissue stromal cells as well as basal and luminal cells express PLD2. PLD2 protein co-expresses with chromogranin A in castrate-resistant PCa tissue. PLD2 inhibition reduces PCa cell viability, colony forming ability and directional cell movement. Conclusions PLD2 expression correlates with increasing Gleason score to GS8. PLD2 inhibition has the potential to reduce PCa progression.

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Section: Introductionmentioning

confidence: 53%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phospholipase D2 in prostate cancer: protein expression changes with Gleason score

et al. 2019

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“…Of them, PCs was abnormally increased by (1.22-7.69) fold after LNCaP cell transition to AIPC 10 . Phospholipase D ( PLD ) is an important enzyme that regulates phospholipid metabolism in the organism 11 , 12 . In mammals, PLD exists in two forms: PLD1 and PLD 2 13 , 14 .…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Phospholipase D1 mRNA Expression Slows Down the Proliferation Rate of Prostate Cancer Cells That Have Transited to Androgen Independence

Shi

et al. 2018

J. Cancer

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To explore the role of phospholipase D1 (PLD1) mRNA in transition of prostate cancer (PCa) cells to androgen independence, we used Arraystar Human LncRNA Microarray V3.0 to detect and compare the differential expression of PLD1 and its signaling pathway-related gene in standard androgen dependence prostate cancer (ADPC) cell line LNCaP before and after the occurrence of androgen independence prostate cancer (AIPC) transition. In addition, we used the shRNA lentiviral vector to inhibit the PLD1 mRNA expression and observed its effect on LNCaP cell proliferation after AIPC transition by using MTS method. The results showed that the expression level of PLD1 mRNA was increased by 373-fold after AIPC transition (P<0.05); the PI3K/AKT signaling pathway-related gene expression was also elevated (P<0.05); the growth rate of LNCaP cells that had transited to androgen independence was reduced by about 30% when the PLD1 mRNA expression was inhibited by the shRNA lentivirus as compared with the negative control group (P<0.05). All these results suggest that PLD1 mRNA and the related PI3K/AKT signaling pathway may play an important role in AIPC. Down-regulating the expression of PLD1 mRNA could to some extent inhibit the proliferation rate of PCa cells after AIPC transition.

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Hypoxia‐induced cofilin 1 promotes hepatocellular carcinoma progression by regulating the PLD1/AKT pathway

Yao

Chen

et al. 2021

Clinical & Translational Med

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Phospholipase D inhibitors reduce human prostate cancer cell proliferation and colony formation

Cited by 41 publications

References 73 publications

Phospholipase D2 in prostate cancer: protein expression changes with Gleason score

Phospholipase D2 in prostate cancer: protein expression changes with Gleason score

Inhibition of Phospholipase D1 mRNA Expression Slows Down the Proliferation Rate of Prostate Cancer Cells That Have Transited to Androgen Independence

Hypoxia‐induced cofilin 1 promotes hepatocellular carcinoma progression by regulating the PLD1/AKT pathway

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