Phospholipase D encapsulated into metal-surfactant nanocapsules for enhancing biocatalysis in a two-phase system

Li, Hui; Pang, Yang; Wang, Xin; Cao, Xun; He, Xun; Chen, Kequan; Li, Ganlu; Ouyang, Pingkai; Tan, Weiming

doi:10.1039/c8ra09827a

Cited by 9 publications

(16 citation statements)

References 38 publications

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“…Equations ( 4)-( 6) have been successfully used for the correlation of binodal data of IL-based ATPSs [28][29][30], polymer-based ATPSs [31,32], and hydrophilic alcohol-based ATPSs [33,34]. Based on the obtained R 2 and sd a values in Tables 3-5, it can be concluded that equations ( 5)-( 7) are is the experimental mass fraction of the alcohol mixture, w cal i is the corresponding data calculated using equation (5), and "N" represents the number of binodal data points. perfectly suitable for correlating the binodal data of the investigated systems.…”

Section: Phase Behaviors Of the Il-salt Ilatpsmentioning

confidence: 99%

“…Since aqueous biphasic systems were proposed by Beijernick in 1896 [3], the aqueous two-phase system (ATPS) as a new technology that is safe and easy to control and has a simple process is now gradually taking the place of traditional organic-water solvent extraction systems. e ATPS is usually composed of two or more polymers, a polymer, and a salt [4,5].…”

Section: Introductionmentioning

confidence: 99%

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The Research and Mechanism of Extracting Vitamin B6 Using Aqueous Two-Phase Systems

Gao

Chen

et al. 2020

Journal of Chemistry

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The aim of this work is to investigate the quaternary systems which contain (polyethylene glycol/isopropyl alcohol) + ethanol + salt (Na2SO4/Na2CO3) + water to understand their liquid-liquid equilibrium (LLE) and salting-out ability and apply them to definite uses. For this purpose, phase diagrams and liquid-liquid equilibrium (LLE) data of the investigated quaternary systems were experimentally determined at 308.15, 318.15, and 328.15 K. Binodal curves were fitted to three empirical nonlinear equations, whereas tie-lines were fitted to the Setschenow-type equation and another two-parameter equation. All the models were successfully correlated with the experimental data. What is more, factors such as temperature, different organic solvent, and type of salt are studied. It was demonstrated that temperature has no influence in binodal curves and the salting-out ability follows the order Na2CO3 > Na2SO4. Finally, we chose the best system (isopropyl alcohol + ethanol + Na2CO3 + H2O) to find out the extraction efficiency when dealing with vitamin B6. The results show that when the temperature is 308.15 K and pH is 5.02, the extraction efficient can reach a top level. It serves as a theoretical basis for the extraction, separation, and purification.

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Section: Phase Behaviors Of the Il-salt Ilatpsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Research and Mechanism of Extracting Vitamin B6 Using Aqueous Two-Phase Systems

Gao

Chen

et al. 2020

Journal of Chemistry

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“…High stirring rate can promote mass transfer in biphasic enzymatic catalysis [47,48]. However, the further increase of stirring rate from 900 to 1000 rpm could result in fracture of the nanocomposite [36,49] and the dissociation of 2-Melm or PLA1, resulting in a significant reduction of catalytic activity. A weakly alkaline environment (pH 8) can promote the catalytic activity of PLA1 and result in higher L-α-GPC yield.…”

Section: Enzymatic Activities Of 2-melm@pla1/msncmentioning

confidence: 99%

“…The enzymeZIF-8 treated with salt ions showed enhanced activity, storage stability, and the ability to resist against trypsin [29]. We have successfully prepared a novel enzyme-metal-surfactant nanocomposite, which demonstrated strong catalytic activity, storage stability, thermal stability, and good reusability in highly polar solvents and deep eutectic solvents [34][35][36]. Under these considerations, we proposed using metal ions and organic surfactants to construct a metal-surfactant nanocomposite capable of simultaneously encapsulating enzymes, followed by subsequent coordinating modification of the nanocomposite with alkalescent 2-Methylimidazole (2-Melm).…”

Section: Introductionmentioning

confidence: 99%

Alkaline Modification of a Metal–Enzyme–Surfactant Nanocomposite to Enhance the Production of L-α-glycerylphosphorylcholine

Cao

et al. 2019

Catalysts

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Microenvironment modification within nanoconfinement can maximize the catalytic activity of enzymes. Phospholipase A1 (PLA1) has been used as the biocatalyst to produce high value L-α-glycerylphosphorylcholine (L-α-GPC) through hydrolysis of phosphatidylcholine (PC). We successfully developed a simple co-precipitation method to encapsulate PLA1 in a metal–surfactant nanocomposite (MSNC), then modified it using alkalescent 2-Methylimidazole (2-Melm) to promote catalytic efficiency in biphasic systems. The generated 2-Melm@PLA1/MSNC showed higher catalytic activity than PLA1/MSNC and free PLA1. Scanning electron microscopy and transmission electron microscopy showed a typical spherical structure of 2-Melm@PLA1/MSNC at about 50 nm, which was smaller than that of 2-Melm@MSNC. Energy disperse spectroscopy, N2 adsorption isotherms, Fourier transform infrared spectrum, and high-resolution X-ray photoelectron spectroscopy proved that 2-Melm successfully modified PLA1/MSNC. The generated 2-Melm@PLA1/MSNC showed a high catalytic rate per unit enzyme mass of 1.58 μmol mg-1 min-1 for the formation of L-α-GPC. The 2-Melm@PLA1/MSNC also showed high thermal stability, pH stability, and reusability in a water–hexane biphasic system. The integration of alkaline and amphiphilic properties of a nanocomposite encapsulating PLA1 resulted in highly efficient sequenced reactions of acyl migration and enzymatic hydrolysis at the interface of a biphasic system, which cannot be achieved by free enzyme.

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“…17,18 The common phospholipases are mainly phospholipase A 1 (PLA 1 ), phospholipase A 2 (PLA 2 ), phospholipase B (PLB), phospholipase C (PLC) and phospholipase D (PLD). [19][20][21][22] Their action sites are shown in Fig. 1.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic preparation of glycerophosphatilcholine catalyzed by combinational phospholipases: a comparative study of concertedversusstepwise catalysis

et al. 2020

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Phospholipase D encapsulated into metal-surfactant nanocapsules for enhancing biocatalysis in a two-phase system

Abstract: PLD@MSNC transforms a two-phase system into an emulsion phase, and enhances transphosphatidylation.

Cited by 9 publications

References 38 publications

The Research and Mechanism of Extracting Vitamin B6 Using Aqueous Two-Phase Systems

The Research and Mechanism of Extracting Vitamin B6 Using Aqueous Two-Phase Systems

Alkaline Modification of a Metal–Enzyme–Surfactant Nanocomposite to Enhance the Production of L-α-glycerylphosphorylcholine

Enzymatic preparation of glycerophosphatilcholine catalyzed by combinational phospholipases: a comparative study of concertedversusstepwise catalysis

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