Phospholipase D

Gómez‐Cambronero, Julian; Keire, Paul

doi:10.1016/s0898-6568(97)00197-6

Cited by 100 publications

(17 citation statements)

References 150 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hence, it is unlikely that increases in [Ca 2ϩ ] cyt are the direct cause of the elevation in PLD activity, although such increases could function upstream in the PLD activation pathway. A further possibility is that PLD is activated by a G protein, as seen in many mammalian PLD-based signal transduction pathways (30). G protein activation of PLD also has been reported in Chlamydomonas (24,43), and there are extensive data implicating G proteins as intermediates in signal transduction in guard cells (40,44).…”

Section: Discussionmentioning

confidence: 92%

See 1 more Smart Citation

Abscisic acid signal transduction in guard cells is mediated by phospholipase D activity

Jacob

Ritchie

Assmann

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

273

228

View full text Add to dashboard Cite

In guard cells, the plant hormone abscisic acid (ABA) inhibits stomatal opening and induces stomatal closure through the coordinated regulation of ion transport. Despite this central role of ABA in regulating stomatal function, the signal transduction events leading to altered ion fluxes remain incompletely understood. We report that the activity of the enzyme phospholipase D (PLD) transiently increased in guard cell protoplasts at 2.5 and 25 min after ABA application. Treatment of guard cell protoplasts with phosphatidic acid (PtdOH), one of the products of PLD activity, led to an inhibition of the activity of the inward K ؉ channel. PtdOH also induced stomatal closure and inhibited stomatal opening when added to epidermal peels. Application of 1-butanol (1-buOH), a selective inhibitor of PtdOH production by PLD, inhibited the increase in PtdOH production elicited by ABA. 1-BuOH treatment also partially prevented ABA-induced stomatal closure and ABA-induced inhibition of stomatal opening. This inhibitory effect of buOH was enhanced by simultaneous application of nicotinamide, an inhibitor of cADP ribose action. These results suggest that in the guard cell, ABA activates the enzyme PLD, which leads to the production of PtdOH. This PtdOH is then involved in triggering subsequent ABA responses of the cell via a pathway operating in parallel to cADP ribose-mediated events.

show abstract

Section: Discussionmentioning

confidence: 92%

“…Phospholipase D (PLD) is involved in signaling in animals, and recent studies suggest a similar role in algae and plants (24)(25)(26)(27)(28)(29)(30)(31). PLD hydrolyzes phospholipids, producing phosphatidic acid (PtdOH) and the head group.…”

mentioning

confidence: 99%

Abscisic acid signal transduction in guard cells is mediated by phospholipase D activity

Jacob

Ritchie

Assmann

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

273

228

View full text Add to dashboard Cite

show abstract

“…The role of PA in VEGF-A signaling still remains to be investigated. In vitro, PA is a regulator of several signaling proteins, some of which are involved in VEGF-A signaling (English, 1996;Gomez-Cambronero and Keire, 1998;Topham and Prescott, 1999). For instance, VEGF-A activates PKCz, which is positively regulated by PA and Src tyrosine phosphorylation (Limatola et al, 1994;Seibenhener et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

Activation of diacylglycerol kinase α is required for VEGF-induced angiogenic signaling in vitro

et al. 2004

View full text Add to dashboard Cite

“…Also, the cells were preincubated with U73122, PAO, RO320432, GO6976, SB203580, or SB202190 for 30 min after being labeled with [ 3 H]palmitic acid and serum-starved for 20 h. After treatment with Der f 2 for 30 min, the cells were quickly washed with ice-cold phosphate-buffered saline and suspended in ice-cold methanol. Lipids were extracted according to the method of Bligh and Dyer (36), and PBt was separated by TLC using a acetate/isooctane/acetic acid/water (110:50:20: 100,v/v) solvent system. The regions corresponding to the authentic PBt bands were identified with 0.002% (w/v) primulin in 80% (v/v) acetone, scraped, and counted using a liquid scintillation counter.…”

Section: Methodsmentioning

confidence: 99%

House Dust Mite Allergen Der f 2-induced Phospholipase D1 Activation Is Critical for the Production of Interleukin-13 through Activating Transcription Factor-2 Activation in Human Bronchial Epithelial Cells

Park

Cho

et al. 2009

Journal of Biological Chemistry

View full text Add to dashboard Cite

The purpose of this study was to identify the role of phospholipase D1 (PLD1) in Der f 2-induced interleukin (IL)-13 production. The major house dust mite allergen, Der f 2, increased PLD activity in human bronchial epithelial cells (BEAS-2B), and dominant negative PLD1 or PLD1 siRNA decreased Der f 2-induced IL-13 expression and production. Treatment of Der f 2 activated the phospholipase C␥ (PLC␥)/protein kinase C␣ (PKC␣)/p38 MAPK pathway. Der f 2-induced PLD activation was attenuated by PLC␥ inhibitors (U73122 and PAO), PKC␣ inhibitors (RO320432 and GO6976), and p38 MAPK inhibitors (SB203580 and SB202190). These results indicate that PLC␥, PKC␣, and p38 MAPK act as upstream activators of PLD in Der f 2-treated BEAS-2B cells. Furthermore, expression and production of IL-13 increased by Der f 2 were also blocked by inhibition of PLC␥, PKC␣, or p38 MAPK, indicating that IL-13 expression and production are related to a PLC␥/PKC␣/p38 MAPK pathway. We found that activating transcription factor-2 (ATF-2) was activated by Der f 2 in BEAS-2B cells and activation of ATF-2 was controlled by PLD1. When ATF-2 activity was blocked with ATF-2 siRNA, Der f 2-induced IL-13 expression and production were decreased. Thus, ATF-2 might be one of the transcriptional factors for the expression of IL-13 in Der f 2-treated BEAS-2B cells. Taken together, PLD1 acts as an important regulator in Der f 2-induced expression and production of IL-13 through activation of ATF-2 in BEAS-2B cells.

show abstract

Phospholipase D

Cited by 100 publications

References 150 publications

Abscisic acid signal transduction in guard cells is mediated by phospholipase D activity

Abscisic acid signal transduction in guard cells is mediated by phospholipase D activity

Activation of diacylglycerol kinase α is required for VEGF-induced angiogenic signaling in vitro

House Dust Mite Allergen Der f 2-induced Phospholipase D1 Activation Is Critical for the Production of Interleukin-13 through Activating Transcription Factor-2 Activation in Human Bronchial Epithelial Cells

Contact Info

Product

Resources

About