Phospholipase C-mediated release of neuraminidase fromTritrichomonas foetus cell surface

Filho, Benedito Prado Dias; Souza, Wanderley de; Andrade, Arnaldo F.B.; Esteves, M. J. G.; Angluster, Jayme

doi:10.1007/bf00937108

Cited by 6 publications

(5 citation statements)

References 29 publications

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“…Numerous immunochemical studies have already been performed on eosinophils to detect the presence of intracellular EDN. Staining was usually preceded by a cell permeabilization step that altered the plasma membrane, leading to a release of endogenous PI‐PLC [25]. This procedure may explain why EDN has not yet been detected as a membrane protein.…”

Section: Discussionmentioning

confidence: 99%

Evidence for glycosylphosphatidylinositol (GPI)‐anchored eosinophil‐derived neurotoxin (EDN) on human granulocytes

Debierre‐Grockiego¹,

Desaint²,

Fuentes³

et al. 2003

FEBS Letters

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Eosinophil-derived neurotoxin (EDN) is one of the four basic proteins stored in speci¢c eosinophil granules. Here we demonstrate that EDN can also be detected at the surface of granulocytes. Reduction of EDN membrane expression after phosphatidylinositol-speci¢c phospholipase C treatment suggests that a glycosylphosphatidylinositol (GPI) anchor is involved in the membrane association of EDN. The presence of a GPI anchor was con¢rmed by a lower expression of membrane EDN on granulocytes from patients with paroxysmal nocturnal hemoglobinuria which present cells lacking GPI anchor proteins. Furthermore, metabolic labeling with GPI anchor components supports biochemical evidence of GPI anchoring of EDN.

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Section: Discussionmentioning

confidence: 99%

Evidence for glycosylphosphatidylinositol (GPI)‐anchored eosinophil‐derived neurotoxin (EDN) on human granulocytes

Debierre‐Grockiego¹,

Desaint²,

Fuentes³

et al. 2003

FEBS Letters

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“…Interestingly, bovine T. foetus also expresses its own sialidase activity in both the plasma membrane of the cell body and flagella. 54 A possible explanation for the presence of both a sialic acid-binding lectin as well as sialidase activity is that bovine T. foetus uses sialic acid as an initial binding site but later cleaves sialic acid enabling trichomonads to contact epithelial cells using other known adhesins. Similar to bovine T. foetus, our unpublished observations suggest that adhesion of feline T. foetus to porcine intestinal epithelial cells also is partially dependent on a sialic acid-based mechanism.…”

Section: Colonization Of the Intestinal Epitheliummentioning

confidence: 99%

“…94,95 Bovine T. foetus is thought to possess endogenous phospholipase C activ- ity. 54 Investigation into the role of phospholipases and porins in both bovine and feline T. foetus infection requires further study.…”

Section: Cytotoxic Effects On Mucosal Epitheliummentioning

confidence: 99%

“…These findings suggest a role for sialic acid in bovine T. foetus adhesion. Interestingly, bovine T. foetus also expresses its own sialidase activity in both the plasma membrane of the cell body and flagella . A possible explanation for the presence of both a sialic acid‐binding lectin as well as sialidase activity is that bovine T. foetus uses sialic acid as an initial binding site but later cleaves sialic acid enabling trichomonads to contact epithelial cells using other known adhesins.…”

Section: Pathogenesis Of Trichomonosismentioning

confidence: 99%

“…Trichomonas vaginalis also secretes porins that exhibit a perforin‐like activity and induce cellular cytotoxicity by creation of pores in erythrocyte membranes in vitro . Bovine T. foetus is thought to possess endogenous phospholipase C activity . Investigation into the role of phospholipases and porins in both bovine and feline T. foetus infection requires further study.…”

Section: Pathogenesis Of Trichomonosismentioning

confidence: 99%

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Mechanisms of Tritrichomonas foetus Pathogenicity in Cats with Insights from Venereal Trichomonosis

Tolbert

Gookin

2016

Veterinary Internal Medicne

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Almost 20 years has passed since trichomonosis was first recognized as a potential cause of diarrhea in domestic cats. Despite progress in confirming disease causation, developing means for diagnosis, and identifying approaches to treatment of the infection, we still know very little about how this parasite causes diarrhea. With increasing recognition of resistance of trichomonosis to treatment with 5‐nitroimidazole drugs, new treatment strategies based on an understanding of disease pathogenesis are needed. In this review, lessons learned from the pathogenesis of venereal trichomonosis in people and cattle are applied to clinical observations of trichomonosis in cats in effort to generate insight into areas where further research may be beneficial.

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Proteomic analysis of proteins released by Tritrichomonas foetus: Identification of potential targets for the development of new diagnostic methods

Abdala

Rivero

Luque

et al. 2023

Veterinary Parasitology

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Phospholipase C-mediated release of neuraminidase fromTritrichomonas foetus cell surface

Cited by 6 publications

References 29 publications

Evidence for glycosylphosphatidylinositol (GPI)‐anchored eosinophil‐derived neurotoxin (EDN) on human granulocytes

Evidence for glycosylphosphatidylinositol (GPI)‐anchored eosinophil‐derived neurotoxin (EDN) on human granulocytes

Mechanisms of Tritrichomonas foetus Pathogenicity in Cats with Insights from Venereal Trichomonosis

Proteomic analysis of proteins released by Tritrichomonas foetus: Identification of potential targets for the development of new diagnostic methods

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