Phospholipase A2 enzyme from the venom of Egyptian honey bee Apis mellifera lamarckii with anti-platelet aggregation and anti-coagulation activities

Darwish, Doaa A.; Masoud, Hassan M. M.; Abdel-Monsef, Mohamed M.; Helmy, Mohamed S.; Zidan, Hind A.; Ibrahim, Mona E.

doi:10.1186/s43141-020-00112-z

Cited by 14 publications

(10 citation statements)

References 45 publications

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“…Likewise, the same study demonstrated that MEL and PLA2 (which are also present in A. mellifera syriaca venom) are mainly responsible for this anticoagulant effect. This study supports another finding that showed that PLA2 from the venom of Egyptian honeybee Apis mellifera lamarckii exerts anticoagulant activities [ 37 ].…”

Section: Discussionsupporting

confidence: 91%

Apis mellifera syriaca Venom: Evaluation of Its Anticoagulant Effect, Proteolytic Activity, and Cytotoxicity along with Its Two Main Compounds—MEL and PLA2—On HeLa Cancer Cells

et al. 2022

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Bee venom (BV) is one of the most remarkable natural products that has been a subject of studies since ancient times. Recent studies have shown that Apis mellifera syriaca venom possesses antibacterial as well as cytotoxic effects on cancer cell lines. The venom contains a variety of bioactive molecules—mainly melittin (MEL) and phospholipase A2 (PLA2), as well as other compounds that are not well characterized. In this work, we continue the biological characterization of A. mellifera syriaca venom by testing its anticoagulant effect on human plasma using the prothrombin time (PT) test, as well as assessing its proteolytic activity. In addition, the cytotoxicity of the crude venom—and of its two main components, MEL and PLA2—was tested on HeLa cancer cell lines for the first time. The results obtained showed the capacity of A. mellifera syriaca venom to increase clotting time, thereby proving its anticoagulant effect. Moreover, the venom did not demonstrate a significant proteolytic activity unless administrated at concentrations ≥ 5 mg/mL. Finally, we showed that crude A. mellifera syriaca venom, along with MEL, exhibit a strong in vitro cytotoxic effect on HeLa cancer cell lines, even at low concentrations. In summary, our findings could serve as a basis for the development of new natural-based drug candidates in the therapeutic field.

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Section: Discussionsupporting

confidence: 91%

Apis mellifera syriaca Venom: Evaluation of Its Anticoagulant Effect, Proteolytic Activity, and Cytotoxicity along with Its Two Main Compounds—MEL and PLA2—On HeLa Cancer Cells

et al. 2022

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“…Mammadova and ShA (2017) purified and identified the components of bee venom using ion-exchange chromatography with a concentration gradient from 0.01 to 1 M sodium chloride solution on a Servacel-52 column and detected bands, including one with a molecular weight of 22 that they classified as glycoprotein. The band with molecular weight ranging from 19 to 14 KDa might be forms of phospholipase A2 (PLA2) (Pucca et al 2019;Darwish et al 2021), and the band with a molecular weight of ~ 3 kDa which was detected by this technique may confirm the presence of melittin in our venom specimens. This result was matched with previous studies which reported that the bulk of insect venoms that sting humans are composed of various chemical combinations.…”

Section: Discussionmentioning

confidence: 69%

Antifungal and antibiofilm activities of bee venom loaded on chitosan nanoparticles: a novel approach for combating fungal human pathogens

El-Didamony

Kalaba

El‐Fakharany

et al. 2022

World J Microbiol Biotechnol

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The prevalence of opportunistic human fungal pathogens is increasing worldwide, and antimicrobial resistance is one of the greatest medical challenges the world faces. Therefore, this study aimed to develop a novel agent to control fungal pathogens. The honeybee products (honey, royal jelly, propolis, bee bread, and bee venom) were screened against unicellular fungal (UCF) pathogens (Cryptococcus neoformans, Kodamaea ohmeri, and Candida albicans) and the bee venom was only exhibited an inhibitory effect against them. The protein contents of crude bee venom were separated using the gel filtration technique into eight fractions which were visualized on sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) to confirm the presence of five bands with molecular weights of 65, 43, 21, 15, and 3 KDa. Bee venom (BV) of Apis mellifera loaded chitosan nanoparticles were prepared by the ionotropic gelation method. The encapsulation efficiency%, average size, zeta potentials, and surface appearance by Transmission electron microscope (TEM) were evaluated for the prepared nanoparticles. The minimum inhibitory concentration (MIC) of crude BV and BV loaded chitosan nanoparticles (BV-CNPs) was evaluated against the offer mentioned UCF where the MIC values of crude BV were 6.25, 3.12 & 6.25 while MIC values in the case of BV-CNPs were decreased to 3.12, 3.12 & 1.56 mg/ml against C. neoformans, K. ohmeri and C. albicans, respectively. Also, the results showed that BV-CNPs suppressed the biofilm formation as well as yeast to hyphal transition formed by the examined UCF. These results revealed that BV-CNPs are a promising natural compound for fungal pathogens treatment. Graphical abstract

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“…PLA2 has a wide range of pharmacological properties, such as the ability to inhibit the growth of cancer cells and to have anti-inflammatory, hepatotoxic, and neuroprotective effects [ 80 , 81 , 82 ]. PLA2 enzyme was purified from A. m. lamarckii venom and displayed anti-coagulant and anti-platelet aggregation activities, making it a promising agent against clot formation [ 83 ].…”

Section: Biological Properties Of Bee Venom From a M Lamarckiimentioning

confidence: 99%

A Spotlight on the Egyptian Honeybee (Apis mellifera lamarckii)

El‐Seedi

El-Wahed

Zhao

et al. 2022

Animals

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Egypt has an ongoing long history with beekeeping, which started with the ancient Egyptians making various reliefs and inscriptions of beekeeping on their tombs and temples. The Egyptian honeybee (Apis mellifera lamarckii) is an authentic Egyptian honeybee subspecies utilized in apiculture. A. m. lamarckii is a distinct honeybee subspecies that has a particular body color, size, and high levels of hygienic behavior. Additionally, it has distinctive characteristics; including the presence of the half-queens, an excessive number of swarm cells, high adaptability to climatic conditions, good resistance to specific bee diseases, including the Varro disorder, and continuous breeding during the whole year despite low productivity, using very little propolis, and tending to abscond readily. This review discusses the history of beekeeping in Egypt and its current situation in addition to its morphology, genetic analysis, and distinctive characters, and the defensive behaviors of native A. m. lamarckii subspecies.

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Phospholipase A2 enzyme from the venom of Egyptian honey bee Apis mellifera lamarckii with anti-platelet aggregation and anti-coagulation activities

Cited by 14 publications

References 45 publications

Apis mellifera syriaca Venom: Evaluation of Its Anticoagulant Effect, Proteolytic Activity, and Cytotoxicity along with Its Two Main Compounds—MEL and PLA2—On HeLa Cancer Cells

Apis mellifera syriaca Venom: Evaluation of Its Anticoagulant Effect, Proteolytic Activity, and Cytotoxicity along with Its Two Main Compounds—MEL and PLA2—On HeLa Cancer Cells

Antifungal and antibiofilm activities of bee venom loaded on chitosan nanoparticles: a novel approach for combating fungal human pathogens

A Spotlight on the Egyptian Honeybee (Apis mellifera lamarckii)

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