Phosphoinositides as Determinants of Membrane Identity, Apicobasal Polarity, and Lumen Formation

Shewan, Annette M.; Awad, Aline; Peng, Juan; Gassama‐Diagne, Ama

doi:10.1007/978-3-319-14463-4_10

Cited by 2 publications

(2 citation statements)

References 154 publications

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“…The cytoskeleton dependent membrane shaping processes control organelles positioning and endosomal sorting to the specific membrane domains that drive apico-basal polarity and lumen formation in epithelia (Bryant and Mostov 2008;Shewan et al 2015). The polarization process of epithelial cells is often accompanied by the formation of primary cilia protruding in the lumen which formation is controlled by polarity components (Bossinger and The septin family is composed of 13 members in mammals, and the complexity of this gene family is increased by the existence of alternate splicing, which dramatically increases the number of isoforms (Fung et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Septin 9 Orients Apico-Basal Polarity Axis and Controls Plasticity Signals

Cai¹,

Peng²,

Omrane³

et al. 2023

Preprint

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The cytoskeleton is a master organizer of cell cortex, organelles positioning and membrane trafficking. Therefore, playing a decisive role in establishment of apico-basal polarity. Septins are critical cytoskeleton components which function in apico-basal polarity remain weakly investigated. Here, using 3D culture system, we demonstrate that septin9 localizes at basolateral membrane (BM). Its depletion induces an inverted polarity phenotype, decreasing -catenin at BM and increasing the expression of the transforming growth factor (TGF) and the epithelial-to-mesenchymal transition (EMT) markers. Similar effects were observed upon deleting its two polybasic domains, and the mutant become cytoplasmic and apical. The cysts with an inverted polarity phenotype display an invasive phenotype, with src and cortactin accumulating at the peripheric membrane. Inhibition of TGF-receptor and RhoA rescues the polarized phenotype. Although the cysts from overexpressed-septin 9, overgrowth and present a filled lumen. Both phenotypes correspond to tumor features. This suggests that septin 9 expression and assembly through the two PB domains are essential for establishing and maintaining apico-basal polarity against tumor development through a mechanism involving TGF-dependent EMT and RhoA activity.

show abstract

Section: Introductionmentioning

confidence: 99%

Septin 9 Orients Apico-Basal Polarity Axis and Controls Plasticity Signals

Cai¹,

Peng²,

Omrane³

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Cytoskeleton-dependent membrane shaping processes control organelle positioning and endosomal sorting to the specific membrane domains that drive apico-basal polarity and lumen formation in epithelia [4,5]. The polarization process of epithelial cells is often accompanied by the formation of primary cilia protruding into the lumen, which is controlled by polarity components [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Septin 9 Orients the Apico–Basal Polarity Axis and Controls Plasticity Signals

Cai

Peng

Omrane

et al. 2023

Cells

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The cytoskeleton is a master organizer of the cellular cortex and membrane trafficking and therefore plays a crucial role in apico–basal polarity. Septins form a family of GTPases that assemble into non-polar filaments, which bind to membranes and recruit cytoskeletal elements such as microtubules and actin using their polybasic (PB) domains, to perform their broad biological functions. Nevertheless, the role of septins and the significance of their membrane-binding ability in apico–basal polarity remains under-investigated. Here, using 3D cultures, we demonstrated that septin 9 localizes to the basolateral membrane (BM). Its depletion induces an inverted polarity phenotype, decreasing β-catenin at BM and increasing transforming growth factor (TGFβ) and Epithelial–Mesenchymal Transition (EMT) markers. Similar effects were observed after deleting its two PB domains. The mutant became cytoplasmic and apical. The cysts with an inverted polarity phenotype displayed an invasive phenotype, with src and cortactin accumulating at the peripheral membrane. The inhibition of TGFβ-receptor and RhoA rescued the polarized phenotype, although the cysts from overexpressed septin 9 overgrew and presented a filled lumen. Both phenotypes corresponded to tumor features. This suggests that septin 9 expression, along with its assembly through the two PB domains, is essential for establishing and maintaining apico–basal polarity against tumor development.

show abstract

Phosphoinositides as Determinants of Membrane Identity, Apicobasal Polarity, and Lumen Formation

Cited by 2 publications

References 154 publications

Septin 9 Orients Apico-Basal Polarity Axis and Controls Plasticity Signals

Septin 9 Orients Apico-Basal Polarity Axis and Controls Plasticity Signals

Septin 9 Orients the Apico–Basal Polarity Axis and Controls Plasticity Signals

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