Phosphoinositide-specific phospholipase C signalling mediates expression of two phenylalanine ammonia lyase genes induced by salicylic acid in Capsicum chinense cells

Rodas-Junco, Beatriz A.; Muñoz-Sánchez, Armando; Poot-Poot, Wilberth Alfredo; Vázquez‐Flota, Felipe; Hernández‐Sotomayor, S.M. Teresa

doi:10.1007/s13562-019-00544-w

Cited by 5 publications

(4 citation statements)

References 10 publications

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“…The order of PAL activity after 1 d of induction was as follows: SA > CD > PEG > C + S > P + C + S > P + C > P + S > C. The highest PAL was observed in the SA treatment, which was 2.59 times higher than that of the Control group (C). Similar results were also reported by Rodas-Junco et al [51,52]. After 5 days of induction, the PAL values of elicitor treatments were significantly higher compared with the Control treatment (p < 0.05), thus indicating constant oxidative stress in suspension cells.…”

Section: Ppo and Pal Activitysupporting

confidence: 90%

Transcriptomics and Physiological Analyses Reveal Changes in Paclitaxel Production and Physiological Properties in Taxus cuspidata Suspension Cells in Response to Elicitors

Zhao,

Zhang,

et al. 2023

Plants

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In this research, the cell growth, physiological, and biochemical reactions, as well as the paclitaxel production, of Taxus cuspidata suspension cells after treatment with polyethylene glycol (PEG), cyclodextrin (CD), or salicylic acid (SA) (alone or in combination) were investigated. To reveal the paclitaxel synthesis mechanism of T. cuspidata suspension cells under elicitor treatment, the transcriptomics of the Control group and P + C + S group (PEG + CD + SA) were compared. The results show that there were no significant differences in cell biomass after 5 days of elicitor treatments. However, the content of hydrogen peroxide (H2O2) and malondialdehyde (MDA), and the activities of phenylalanine ammonia-lyase (PAL) and polyphenol oxidase (PPO) after elicitor combination treatments were decreased compared with the single-elicitor treatment. Meanwhile, the antioxidant enzyme activity (superoxide dismutase (SOD), catalase (CAT), and peroxidase (PO)) and the contents of soluble sugar and soluble protein were increased after combination elicitor treatments. Additionally, the paclitaxel yield after treatment with the combination of all three elicitors (P + C + S) was 6.02 times higher than that of the Control group, thus indicating that the combination elicitor treatments had a significant effect on paclitaxel production in T. cuspidata cell suspension culture. Transcriptomics analysis revealed 13,623 differentially expressed genes (DEGs) between the Control and P + C + S treatment groups. Both GO and KEGG analyses showed that the DEGs mainly affected metabolic processes. DEGs associated with antioxidant enzymes, paclitaxel biosynthesis enzymes, and transcription factors were identified. It can be hypothesized that the oxidative stress of suspension cells occurred with elicitor stimulation, thereby leading to a defense response and an up-regulation of the gene expression associated with antioxidant enzymes, paclitaxel synthesis enzymes, and paclitaxel synthesis transcription factors; this ultimately increased the production of paclitaxel.

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Section: Ppo and Pal Activitysupporting

confidence: 90%

Transcriptomics and Physiological Analyses Reveal Changes in Paclitaxel Production and Physiological Properties in Taxus cuspidata Suspension Cells in Response to Elicitors

Zhao,

Zhang,

et al. 2023

Plants

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“…Recently, in 2019, we reported the role of the PI-PLC pathway in the transcriptional regulation of CchPAL1 and CchPAL5, which are putative PAL genes in Capsicum (Figure 3). We observed that an increase in phosphoinositide levels appears to be important in the C. chinense SA-specific response, with PI-PLC signaling contributing mainly to a common SA response [41]. In contrast, when PLD/PA synthesis is blocked by 1-butanol in cells stimulated with SA, the transcriptional regulation of CchPAL1 and CchPAL5 in response to SA does not involve the PLD pathway.…”

Section: Salicylic Acid Phospholipid Signaling and Secondary Metabolmentioning

confidence: 78%

“…In this context, the use of plants cells as a simplified and amenable experimental model has allowed the study of SA-induced signaling mechanisms that would be too complex in plant tissues or organs [40]. Several lines of evidence using plant cells suggest that signaling pathways components such as reactive oxygen species (ROS), abscisic acid (ABA), Ca 2+ and phospholipids interact with the SA signaling pathway [2,23,24,41]. However, compared to those involved in biotic stress, the molecular mechanisms involved in abiotic stress in response to SA remain poorly explored.…”

Section: Sa As a Mitigator Of Abiotic Stressmentioning

confidence: 99%

Phospholipid Signaling Is a Component of the Salicylic Acid Response in Plant Cell Suspension Cultures

Rodas-Junco

Nic-Can

Muñoz-Sánchez

et al. 2020

IJMS

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Salicylic acid (SA) is an important signaling molecule involved in plant defense. While many proteins play essential roles in SA signaling, increasing evidence shows that responses to SA appear to involve and require lipid signals. The phospholipid-generated signal transduction involves a family of enzymes that catalyze the hydrolysis or phosphorylation of phospholipids in membranes to generate signaling molecules, which are important in the plant cellular response. In this review, we focus first, the role of SA as a mitigator in biotic/abiotic stress. Later, we describe the experimental evidence supporting the phospholipid–SA connection in plant cells, emphasizing the roles of the secondary lipid messengers (phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidic acid (PA)) and related enzymes (phospholipase D (PLD) and phospholipase C (PLC)). By placing these recent finding in context of phospholipids and SA in plant cells, we highlight the role of phospholipids as modulators in the early steps of SA triggered transduction in plant cells.

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“…Deletion of Arabidopsis AtPLC2, increases the transcript levels of genes in growth hormone synthesis and growth hormone signaling leading to elevated growth hormone levels, which leads to defects in male and female gametophyte development (Li et al, 2015). In addition, CaM, G protein, abscisic acid (ABA), salicylic acid (SA), indole-3-acetic acid (IAA) and phosphorylation have been found to regulate PI-PLC activity to function (Zhu et al, 2021;Rodas-Junco et al, 2020;Takac et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification, Evolution Analysis of PI-PLC Family and Their Expression Patterns in Response to Different Hormones and Growth in Banana (Musa L.)

Fan

et al. 2022

Preprint

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PI-PLC is not only involved in the regulation of plant responses to a variety of biotic and abiotic stresses, but also plays an important role in the regulation of plant growth. banana is one of the four most famous fruits in the world, as well as one of the most important tropical fruits in the world. However, in recent years, blight and cold damage have become two major deadly hazards for banana industry, making it particularly important to improve the resistance of bananas to stress. In this study. PI-PLCs gene family of banana encodes 567–698 amino acidsand. It is mainly located in chloroplasts, mitochondria and cytoplasm. Systematic evolution and homology analysis, PI-PLCs gene family of banana is highly clustered, the structure and function are relatively consistent. There were hormones and stress response elements such as methyl jasmonate, auxin and gibberellin, in the promoter region of MaPLCs family members. The proteins coded by all MaPLCs could interact and participate in a series of life-sustaining activities. The study showed that members of the MaPLCs gene family of bananas could be induced to express by auxin, abscisic acid and salicylic acid. And the leaves appeared curl, wilting symptoms, and water-stained spots.

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Phosphoinositide-specific phospholipase C signalling mediates expression of two phenylalanine ammonia lyase genes induced by salicylic acid in Capsicum chinense cells

Cited by 5 publications

References 10 publications

Transcriptomics and Physiological Analyses Reveal Changes in Paclitaxel Production and Physiological Properties in Taxus cuspidata Suspension Cells in Response to Elicitors

Transcriptomics and Physiological Analyses Reveal Changes in Paclitaxel Production and Physiological Properties in Taxus cuspidata Suspension Cells in Response to Elicitors

Phospholipid Signaling Is a Component of the Salicylic Acid Response in Plant Cell Suspension Cultures

Genome-Wide Identification, Evolution Analysis of PI-PLC Family and Their Expression Patterns in Response to Different Hormones and Growth in Banana (Musa L.)

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