Phosphoinositid signal pathway mediate neurite outgrowth in PC12 cells by staurosporine

Abstract

Objectives:In previous studies, we showed that staurosporine uses intracellular calcium ions to affect cell death in PC12 cells. The bulk release of intracellular excessive Ca 2+ from intracellular sources into cytosol contributes to neuronal apoptotic events, which in turn results in neuronal cell death. However, the mechanisms of Ca 2+ -induced neuronal cell death or neurite elongation is still unclear. Therefore, we investigated the relation between phosphoinositid signal pathway, intracellular calcium, and reactive oxygen species on one hand, with staurosporine-induced neurite outgrowth in PC12 cells on the other. Results: The inhibition of phospholipase C or IP3 receptor antagonist or phosphoinositid signal transduction antagonist produced cell death and suppressed neurite outgrowth by staurosporine in PC12 cells. The inhibition of these enzymes and pathway results in an increase in intracellular Ca 2+ although subsequent hydroxyl radical (•OH) production began after inhibitors exposure.•OH production was signifi cantly attenuated in inhibitor supplemented medium treatment, and it was dependent on the intracellular Ca 2+ concentration. These data indicate that staurosporine activates phosphoinositid signal pathway while endoplasmic Ca 2+, and subsequent •OH production are critical events in staurosporine-induced neurite outgrowth in PC12 cells. Conclusion:We conclude that the fact that staurosporine mobilizes Ca2+ , probably via activating the subcellular compartment, is responsible for staurosporine-induced (Ca2+]i increase during neurite outgrowth in PC12 cells (Fig. 7, Ref. 30). Text in PDF www.elis.sk.

Inhibitory effects of mouse bone marrow mesenchymal stem cell soup on staurospurine-induced cell death in MCF-7 and AGS

“…Recently, we reported that inhibition of IP3R and PLCγ led to attenuating of the rise in intracellular calcium triggered by staurosporine, thus strongly suggesting that ROS generation could potentiate the observed intracellular calcium overload (67). Our data showed that saturospurine at 1μM concentration induced cell death and reduced cell viability by increasing the NO secretion.…”

Regulatory networks upon neurogenesis induction in PC12 cell line by small molecules

“…Cell culture conditions and the selection of the STS concentration were essentially performed according to Zhaleh et al studies (Zhaleh, Azadbakht, & Pour, 2011;Zhaleh, Azadbakht, & Pour, 2012;Zhaleh, Azadbakht, & Pour, 2014). The PC12 cells were cultured in RPMI-1640 culture medium (Gibco, Dublin, Ireland) in the presence of 1% NEAA (nonessential amino acid), and 1% antibiotic (penicillinstreptomycin, Sigma) in 25 ml tissue culture flasks.…”

“…However, apoptosis has also been observed under this condition. Measurement of total neurite length, the fraction of cell differentiation, and the percentages of apoptosis were reported in the previous works (Zhaleh et al, 2011(Zhaleh et al, , 2012(Zhaleh et al, , 2014.…”