Phosphoglycerolipid Signaling in Response to Hormones Under Stress

Pokotylo, Igor; Janda, Martin; Kalachova, Tetiana; Zachowski, Alain; Ruelland, Éric

doi:10.1002/9781118889022.ch22

Cited by 3 publications

(3 citation statements)

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“…DGK genes in plants have been known as key players at the hub of cell signaling and responses to diverse stimuli (Merida et al ., 2008; Tan et al ., 2018). The AtDGK7 gene is known to show tissue‐/stimulus‐specific expression (Gomez‐Merino et al ., 2005; Pokotylo et al ., 2017). We detected two independent DNase‐hypersensitive sites (DHSs) in the 1.5‐kb upstream region of AtDGK7 , which were named as CREα and CREβ , respectively (Figure 1).…”

Section: Resultsmentioning

confidence: 99%

“…A series of DNA fragments from the 1.8‐kb upstream region of AtDGK7 were fused to the LUC reporter, including different combinations of CREα , CREβ , and γ (−1772/−774, a 1‐kb non‐DHS sequence located upstream of CREβ ) (Figure 2a). The mini35S (−50 to −2 bp) promoter was used as a negative control, and the full 35S promoter and the promoter of the RD29A gene in A. thaliana (Pokotylo et al ., 2017) were used as positive controls. The RD29A promoter has been well characterized and is an abiotic stress‐responsive promoter derived from the ‘responsive to desiccation 29A’ gene ( RD29A , AT5G52310) (Yamaguchi‐Shinozaki and Shinozaki, 1994).…”

Section: Resultsmentioning

confidence: 99%

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Enhancer‐mediated reporter gene expression in Arabidopsis thaliana: a forward genetic screen

et al. 2021

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Gene expression is controlled and regulated by interactions between cis-regulatory DNA elements (CREs) and regulatory proteins. Enhancers are one of the most important classes of CREs in eukaryotes. Eukaryotic genes, especially those related to development or responses to environmental cues, are often regulated by multiple enhancers in different tissues and/or at different developmental stages. Remarkably, little is known about the molecular mechanisms by which enhancers regulate gene expression in plants. We identified a distal enhancer, CREb, which regulates the expression of AtDGK7, which encodes a diacylglycerol kinase in Arabidopsis. We developed a transgenic line containing the luciferase reporter gene (LUC) driven by CREb fused with a minimal cauliflower mosaic virus (CaMV) 35S promoter. The CREb enhancer was shown to play a role in the response to osmotic pressure of the LUC reporter gene. A forward genetic screen pipeline based on the transgenic line was established to generate mutations associated with altered expression of the LUC reporter gene. We identified a suite of mutants with variable LUC expression levels as well as different segregation patterns of the mutations in populations. We demonstrate that this pipeline will allow us to identify trans-regulatory factors associated with CREb function as well as those acting in the regulation of the endogenous AtDGK7 gene.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Enhancer‐mediated reporter gene expression in Arabidopsis thaliana: a forward genetic screen

et al. 2021

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“…Phosphatidic acid acts as a signaling molecule by binding to target proteins altering their subcellular localization and enzymatic activity. This signaling pathway is activated during plant exposure to both biotic and abiotic stresses ( Pokotylo et al, 2017 ). The hormone signaling pathways functions systemically in host plants, thus labeled as the systemic acquired resistance (SAR) effective against a wide range of pathogens.…”

Section: Introductionmentioning

confidence: 99%

Current Status of the Disease-Resistant Gene(s)/QTLs, and Strategies for Improvement in Brassica juncea

2021

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Brassica juncea is a major oilseed crop in tropical and subtropical countries, especially in south-east Asia like India, China, Bangladesh, and Pakistan. The widespread cultivation of genetically similar varieties tends to attract fungal pathogens which cause heavy yield losses in the absence of resistant sources. The conventional disease management techniques are often expensive, have limited efficacy, and cause additional harm to the environment. A substantial approach is to identify and use of resistance sources within the Brassica hosts and other non-hosts to ensure sustainable oilseed crop production. In the present review, we discuss six major fungal pathogens of B. juncea: Sclerotinia stem rot (Sclerotinia sclerotiorum), Alternaria blight (Alternaria brassicae), White rust (Albugo candida), Downy mildew (Hyaloperonospora parasitica), Powdery mildew (Erysiphe cruciferarum), and Blackleg (Leptoshaeria maculans). From discussing studies on pathogen prevalence in B. juncea, the review then focuses on highlighting the resistance sources and quantitative trait loci/gene identified so far from Brassicaceae and non-filial sources against these fungal pathogens. The problems in the identification of resistance sources for B. juncea concerning genome complexity in host subpopulation and pathotypes were addressed. Emphasis has been laid on more elaborate and coordinated research to identify and deploy R genes, robust techniques, and research materials. Examples of fully characterized genes conferring resistance have been discussed that can be transformed into B. juncea using advanced genomics tools. Lastly, effective strategies for B. juncea improvement through introgression of novel R genes, development of pre-breeding resistant lines, characterization of pathotypes, and defense-related secondary metabolites have been provided suggesting the plan for the development of resistant B. juncea.

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Is the PLC pathway involved in the response to phenol treatment in tobacco hairy roots?

Alderete

Racagni

Agostini

et al. 2019

In Vitro Cell.Dev.Biol.-Plant

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Phosphoglycerolipid Signaling in Response to Hormones Under Stress

Cited by 3 publications

References 184 publications

Enhancer‐mediated reporter gene expression in Arabidopsis thaliana: a forward genetic screen

Enhancer‐mediated reporter gene expression in Arabidopsis thaliana: a forward genetic screen

Current Status of the Disease-Resistant Gene(s)/QTLs, and Strategies for Improvement in Brassica juncea

Is the PLC pathway involved in the response to phenol treatment in tobacco hairy roots?

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