Phosphoglycerate Kinase Is Involved in Carbohydrate Utilization, Extracellular Polysaccharide Biosynthesis, and Cell Motility of Xanthomonas axonopodis pv. glycines Independent of Clp

Guo, Wei; Gao, Jie; Wang, Hongjie; Su, Ru-Yi; Sun, Chu-Yun; Gao, Si-Han; Liu, Jianzhong; Chen, Gongyou

doi:10.3389/fmicb.2020.00091

Cited by 10 publications

(10 citation statements)

References 38 publications

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“…In contrast, the heterological expression levels of glyceraldehyd-3-phosphate dehydrogenase and phosphoglycerate kinase, related to EPS biosynthesis of Xanthomonas axonopodis pv . Glycines) [114] , decreased under osmotic stress [115] . In general, environmental stress can alter the expression of genes involved in EPS biosynthesis.…”

Section: Changes the Expression Of Genes Involved In Eps Production Umentioning

confidence: 94%

Exopolysaccharide production by lactic acid bacteria: the manipulation of environmental stresses for industrial applications

Nguyen¹,

Nguyen²,

Bui³

et al. 2020

AIMS Microbiology

121

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Exopolysaccharides (EPSs) are biological polymers secreted by microorganisms including Lactic acid bacteria (LAB) to cope with harsh environmental conditions. EPSs are one of the main components involved in the formation of extracellular biofilm matrix to protect microorganisms from adverse factors such as temperature, pH, antibiotics, host immune defenses, etc.. In this review, we discuss EPS biosynthesis; the role of EPSs in LAB stress tolerance; the impact of environmental stresses on EPS production and on the expression of genes involved in EPS synthesis. The evaluation results indicated that environmental stresses can alter EPS biosynthesis in LAB. For further studies, environmental stresses may be used to generate a new EPS type with high biological activity for industrial applications.

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Section: Changes the Expression Of Genes Involved In Eps Production Umentioning

confidence: 94%

Exopolysaccharide production by lactic acid bacteria: the manipulation of environmental stresses for industrial applications

Nguyen¹,

Nguyen²,

Bui³

et al. 2020

AIMS Microbiology

121

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“…Xag enters soybean leaves through stomata and wounds, and reproduces within the intercellular spaces of the spongy mesophyll, resulting in erumpent pustules surrounded by yellow halos 6 . Xag can be spread by rain droplets during the growing season and by seed transportation 7 . A number of spreads of bacterial pustule disease have taken place in Asia during recent years, reducing the size and quantity of the seeds 8,9 …”

Section: Introductionmentioning

confidence: 99%

“…6 Xag can be spread by rain droplets during the growing season and by seed transportation. 7 A number of spreads of bacterial pustule disease have taken place in Asia during recent years, reducing the size and quantity of the seeds. 8,9 Flavonoids rutin and genistein have been directly related to the defence mechanism of soybean to Xag, and soybean resistant varieties are known to produce higher amounts of flavonoids in comparison with susceptible varieties.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial mechanism of Biochanin A and its efficacy for the control of Xanthomonas axonopodispv.glycines in soybean

Shi

et al. 2020

Pest Management Science

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BACKGROUND Xanthomonas axonopodis pv. glycines (Xag) is a hazardous pathogen able to cause bacterial pustule disease in soybean, reducing crop yield and quality. Although flavonoids rutin and genistein are known to play an important role in soybean defence, soybean is only able to produce Biochanin A in low concentration. RESULTS In this work, Biochanin A was found to produce higher antibacterial activity against Xag in comparison with genistein (minimum inhibitory concentration < 100 μg/mL). Biochanin A was able to inhibit DNA synthesis and flagella formation in Xag, and altered the composition of the bacterial membrane. These effects reduced swimming motility, extracellular protease activity and biofilm formation. Further, Biochanin A was tested for the control of Xag in soybean leaves, showing similar, or even higher, inhibitory ability in comparison with some products commonly used for the control of this pathogen. CONCLUSIONS The antibacterial properties of Biochanin A against Xag have been studied for the first time, revealing new insights on the potential applications of this isoflavonoid for the management of bacterial pustule disease. © 2020 Society of Chemical Industry

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“…Extracellular polysaccharide has long been recognized as an important virulence factor contributing to Xanthomonas pathogenesis ( Guo et al, 2020 ; Li et al, 2020 ). On the minimal NY medium plates, the colonies produced by the three pathogenic wild-type RS105, CRΔ vemR , and CRΔ vemR D 56E strains were similar in size to the non-pathogenic RΔ vemR and CRΔ vemR D 56V strains, albeit mucoid in appearance.…”

Section: Resultsmentioning

confidence: 99%

“…It is axiomatic to believe that the availability, acquisition, and utilization of host cell-derived carbohydrates will have a significant impact on bacterial virulence and pathogenicity through carbon provision and regulation of biosynthesis in EPS ( Guo et al, 2012 , 2015 , 2017 , 2020 ; Cai et al, 2014 ) and DSF for QS ( Zhou et al, 2015 ; Guo et al, 2017 ). Earlier work in our laboratory indicated that (a) glycolysis/gluconeogenesis enzyme fructose-bisphophate aldolase (FbaB) was required for EPS production, virulence, and growth of the Xoc strain RS105 in rice, and (b) fbaB expression was negatively and differentially regulated by both HrpG and HrpX ( Guo et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Xanthomonas oryzae Pv. oryzicola Response Regulator VemR Is Co-opted by the Sensor Kinase CheA for Phosphorylation of Multiple Pathogenicity-Related Targets

Cai

Zou

et al. 2022

Front. Microbiol.

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Two-component systems (TCSs) (cognate sensor histidine kinase/response regulator pair, HK/RR) play a crucial role in bacterial adaptation, survival, and productive colonization. An atypical orphan single-domain RR VemR was characterized by the non-vascular pathogen Xanthomonas oryzae pv. oryzicola (Xoc) is known to cause bacterial leaf streak (BLS) disease in rice. Xoc growth and pathogenicity in rice, motility, biosynthesis of extracellular polysaccharide (EPS), and the ability to trigger HR in non-host tobacco were severely compromised in the deletion mutant strain RΔvemR as compared to the wild-type strain RS105. Site-directed mutagenesis and phosphotransfer experiments revealed that the conserved aspartate (D56) residue within the stand-alone phosphoacceptor receiver (REC) domain is essential for phosphorelay and the regulatory activity of Xoc VemR. Yeast two-hybrid (Y2H) and co-immunoprecipitation (co-IP) data identified CheA as the HK co-opting the RR VemR for phosphorylation. Affinity proteomics identified several downstream VemR-interacting proteins, such as 2-oxoglutarate dehydrogenase (OGDH), DNA-binding RR SirA, flagellar basal body P-ring formation protein FlgA, Type 4a pilus retraction ATPase PilT, stress-inducible sensor HK BaeS, septum site-determining protein MinD, cytoskeletal protein CcmA, and Type III and VI secretion system proteins HrpG and Hcp, respectively. Y2H and deletion mutant analyses corroborated that VemR interacted with OGDH, SirA, FlgA, and HrpG; thus, implicating multi-layered control of diverse cellular processes including carbon metabolism, motility, and pathogenicity in the rice. Physical interaction between VemR and HrpG suggested cross-talk interaction between CheA/VemR- and HpaS/HrpG-mediated signal transduction events orchestrating the hrp gene expression.

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Phosphoglycerate Kinase Is Involved in Carbohydrate Utilization, Extracellular Polysaccharide Biosynthesis, and Cell Motility of Xanthomonas axonopodis pv. glycines Independent of Clp

Cited by 10 publications

References 38 publications

Exopolysaccharide production by lactic acid bacteria: the manipulation of environmental stresses for industrial applications

Exopolysaccharide production by lactic acid bacteria: the manipulation of environmental stresses for industrial applications

Antibacterial mechanism of Biochanin A and its efficacy for the control of Xanthomonas axonopodispv.glycines in soybean

Xanthomonas oryzae Pv. oryzicola Response Regulator VemR Is Co-opted by the Sensor Kinase CheA for Phosphorylation of Multiple Pathogenicity-Related Targets

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