Phosphoglycerate kinase 1 as a promoter of metastasis in colon cancer

Ahmad, Sufian S.; Glatzle, Jörg; Bajaeifer, Khaled; Bühler, Sarah; Lehmann, Thorsten G.; Königsrainer, Ingmar; Vollmer, Jörg-Peter; Sipos, Bence; Ahmad, Suhaib S; Northoff, Hinnak; Königsrainer, Alfred; Zieker, Derek

doi:10.3892/ijo.2013.1971

Cited by 88 publications

(69 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The plasmid pBS 16 114b:1, harboring the HPV16 114/b wt genome, was kindly provided by M. Dürst (40), and plasmid hPGK1 pEF-IRES-P, encoding the cDNA for human phosphoglycerate kinase 1 (PGK1), was kindly provided by D. Zieker (41). The HPV16 E8Ϫ and E8 KWK mt genomes were constructed by overlap extension PCR using specific primers and verified by DNA sequencing.…”

Section: Methodsmentioning

confidence: 99%

The Viral E8^E2C Repressor Limits Productive Replication of Human Papillomavirus 16

Straub

Dreer

Fertey

et al. 2014

J Virol

View full text Add to dashboard Cite

Productive replication of human papillomavirus type 16 (HPV16) occurs only in differentiated keratinocyte cells. In addition to the viral E2 activator protein, HPV16 and related HPV types express transcripts coding for an E8^E2C fusion protein, which limits genome replication in undifferentiated keratinocytes. To address E8^E2C's role in productive replication of HPV16, stable keratinocyte cell lines containing wild-type (wt), E8^E2C knockout (E8؊), or E8 KWK mutant (mt) genomes, in which conserved E8 residues were inactivated, were established. Copy numbers of E8؊ and E8 KWK mt genomes and amounts of early and late viral transcripts were greatly increased compared to those for the wt in undifferentiated keratinocytes, suggesting that HPV16 E8^E2C activities are highly dependent upon the E8 part. Upon differentiation in organotypic cultures, E8 mt genomes displayed higher early viral transcript levels, but no changes in cellular differentiation or virus-induced cellular DNA replication in suprabasal cells were observed. E8 mt genomes were amplified to higher copy numbers and showed increased L1 transcripts compared to wt genomes. Furthermore, the number of cells expressing the viral late protein E4 or L1 or amplifying viral genomes was greatly increased in E8 mt cell lines. In wild-type cells, E8^E2C transcript levels did not decrease by differentiation. Our data indicate that the E8^E2C repressor limits viral transcription and replication throughout the complete life cycle of HPV16. P ersistent infections with high-risk (HR) human papillomaviruses (HPV) are a necessary risk factor for the development of cancer of the cervix uteri and are also responsible for a fraction of anal, vaginal, penile, and oropharyngeal cancers (1-3). Notably, HPV16 is an extremely powerful human carcinogen that is responsible for approximately 50% of cervical cancers and is also represented in other HR-HPV-related cancers at high frequencies (2).HPV have a double-stranded DNA genome that is covalently closed and organized in nucleosomes. HPV possess eight to nine open reading frames (E1, E2, E4 to E8, L1, and L2) that are transcribed into polycistronic messages which are further processed by alternative splicing (4). It is currently assumed that HR-HPV encode at least 10 different viral proteins.The HR-HPV replication cycle is tightly linked to the differentiation state of the infected epithelium. In undifferentiated basalcell-like cells, HR-HPV genomes are maintained in the nucleus at 10 to 100 extrachromosomal copies per cell, and only early genes, mainly transcribed from the major early viral promoter located immediately upstream of the E6 gene (P97 for HPV16), but not the late viral L1 and L2 genes are expressed (5). Replication of the viral genome in undifferentiated cells requires the viral E1 and E2 proteins (6). E1 and E2 are sequence-specific DNA binding proteins which form a complex that recognizes the viral origin of replication (6). E1 assembles into a hexamer that acts as the replicative helicase and unwinds the viral DNA...

show abstract

Section: Methodsmentioning

confidence: 99%

The Viral E8^E2C Repressor Limits Productive Replication of Human Papillomavirus 16

Straub

Dreer

Fertey

et al. 2014

J Virol

View full text Add to dashboard Cite

show abstract

“…10 PGK1 has been associated with carcinogenesis and metastasis in various malignancies. 11,12 This has prompted investigations into PGK1 as a potential oncogenic molecular biomarker and an indicator of poor prognosis in pancreatic cancer, 13 gastric cancer, 14 neuroblastoma, 15 and hepatocellular carcinoma. 16 A recent study revealed that PGK1 was a prognostic biomarker of chemoresistance to paclitaxel treatment in patients with breast cancer.…”

Section: Introductionmentioning

confidence: 99%

Down-Regulated Phosphoglycerate Kinase 1 Expression Is Associated With Poor Prognosis in Patients With Gallbladder Cancer

et al. 2015

View full text Add to dashboard Cite

show abstract

“…In our surgical samples we have identified three distinct isoforms of PGK1 all increased with respect to the normal tissues. Many types of cancers, including those of the breast [47], the colon [48], the liver [49] and others, have been shown to exhibit an increased expression of PGK1. Indeed, it has been demonstrated that, in addition to its role as a glycolytic enzyme, PGK1 may act as a promoter of cancer progression and induce chemoresistance for some type of drugs [47,48].…”

Section: Phosphoglycerate Kinase 1 Pgk1 (Pgk1)mentioning

confidence: 99%

Retrospective Proteomic Screening of 100 Breast Cancer Tissues

Pucci‐Minafra¹,

Cara²,

Musso³

et al. 2017

Preprint

View full text Add to dashboard Cite

Abstract:The present investigation has been conducted on one hundred tissue fragments of breast cancer, collected and immediately cryopreserved following the surgical resection. The fragments were selected from patients with invasive ductal carcinoma of the breast, the most common and potentially aggressive type of mammary cancer, with the objective to increase the knowledge of breast cancer molecular markers, useful for diagnostic and prognostic categorization of patients, in assessing postsurgical therapeutic regimes. The proteomic screening, by 2D-IPG and mass spectrometry, allowed us to identify two main classes of protein clusters: proteins expressed ubiquitously at high levels in all patients, and proteins expressed sporadically among the same patients. Within the group of ubiquitous proteins, glycolytic enzymes and proteins with anti-apoptotic activity were predominant. Among the sporadic ones, proteins involved in cell motility, molecular chaperones and proteins involved in the detoxification appeared prevalent. The data of the present study indicates that the primary tumor growth is generally supported by two concurrent pathways: the inhibition of apoptosis and the stimulation of cellular proliferation. The second phase of the evolution of the tumor can be prematurely scheduled by the occasional presence of proteins involved in cell motility and in the defenses of the oxidative stress. To our knowledge this report on large-scales proteomics of breast cancer is currently a unique approach in the literature that offers the opportunity to evaluate the presence and recurrence of proteins to be used as prognostic indicators and susceptibility to metastasis in patients operated on for invasive ductal carcinoma of the breast.

show abstract

Phosphoglycerate kinase 1 as a promoter of metastasis in colon cancer

Cited by 88 publications

References 33 publications

The Viral E8^E2C Repressor Limits Productive Replication of Human Papillomavirus 16

The Viral E8^E2C Repressor Limits Productive Replication of Human Papillomavirus 16

Down-Regulated Phosphoglycerate Kinase 1 Expression Is Associated With Poor Prognosis in Patients With Gallbladder Cancer

Retrospective Proteomic Screening of 100 Breast Cancer Tissues

Contact Info

Product

Resources

About