Phosphoglucomutase 1 contributes to optimal cyst development in Toxoplasma gondii

Quach, Emily V.; Cao, Binh; Babacarkhial, Edres; Ho, Daniel Chi Wing; Sharma, Janak; Guiton, Pascale S.

doi:10.1186/s13104-022-06073-5

Cited by 2 publications

(1 citation statement)

References 32 publications

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“…Recent work has also demonstrated that loss of phosphoglucomutase 1, an enzyme related to AG metabolism, results in smaller cysts (70). Our prior work established that bradyzoite replication within tissue cysts occurs in a cyclical pattern that is reflected in the packing density of bradyzoites within the cyst (7).…”

Section: Resultsmentioning

confidence: 99%

TgLaforin, a glucan phosphatase, reveals the dynamic role of storage polysaccharides inToxoplasma gondiitachyzoites and bradyzoites

Murphy,

Troublefield,

Miracle

et al. 2023

Preprint

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The asexual stages ofToxoplasma gondiiare defined by the rapidly growing tachyzoite during the acute infection and by the slow growing bradyzoite housed within tissue cysts during the chronic infection. These stages represent unique physiological states, each with distinct glucans reflecting differing metabolic needs. A defining feature ofT. gondiibradyzoites is the presence of insoluble storage glucans known as amylopectin granules (AGs) that are believed to play a role in reactivation, but their functions during the chronic infection remain largely unexplored. More recently, the presence of storage glucans has been recognized in tachyzoites where their precise function and architecture have yet to be fully defined. Importantly, theT. gondiigenome encodes activities needed for glucan turnover: a glucan phosphatase (TgLaforin; TGME49_205290) and a glucan kinase (TgGWD; TGME49_214260) that catalyze a cycle of reversible glucan phosphorylation required for glucan degradation by amylases. The expression of these enzymes in tachyzoites supports the existence of a storage glucan, evidence that is corroborated by specific labeling with the anti-glycogen antibody IV58B6. Disruption of reversible glucan phosphorylation via a CRISPR/Cas9 knockout (KO) of TgLaforin revealed no growth defects under nutrient-replete conditions in tachyzoites. However, the growth of TgLaforin-KO tachyzoites was severely stunted when starved of glutamine, even under glucose replete conditions. The loss of TgLaforin also resulted in the attenuation of acute virulence in mice accompanied by a lower cyst burden. Defective cyst formation due to profound changes in AG morphology was also observed in TgLaforin-KO parasites, bothin vitroandin vivo. Together, these data demonstrate the importance of glucan turnover across theT. gondiiasexual cycle. These findings, alongside our previously identified class of small molecules that inhibit TgLaforin, implicate reversible glucan phosphorylation as a legitimate target for the development of new drugs against chronicT. gondiiinfections.

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Section: Resultsmentioning

confidence: 99%