Phosphofructokinase (PFK) isozymes in man

Abstract

Isozymic heterogeneity of human phosphofructokinase was investigated by means of ATP inhibition, immunoneutralization by antihuman muscle-type and antiliver-type phosphofructokinase antisera, solubility in (NH4)2SO4 solutions, and starch gel and polyacrylamide slab gel electrophoresis. The enzymes studied by these methods were purified from various normal and malignant human adult tissues by chromatography on blue Dextran Sepharose 4 B columns. From the results of these studied we suggest that three basic phosphofructokinase isozymes could exist: muscle-type, fibroblast-type, and liver-type isozymes. Muscle-type isozyme is the single form found in adult muscle, and is involved in the enzymes from heart, brain, red cell, and testis. Fibroblast-type isozyme is found mainly in the placenta, fibroblasts kidney, and some malignant tissues. Liver-type phosphofructokinase seems to be very definitely the predominant form in mature polymorphonuclear cells, platelets, and liver. Testis and red cell phosphofructokinase enzymes definitely include msucle-type aand liver-type subunits, associated in various hybrid forms.

Heterogeneity of the molecular lesions in inherited phosphofructokinase deficiency.

“…We and others have previously demonstrated the existence of a multilocus isozyme system for human PFK (3)(4)(5)(6). Three structural loci code for muscle (M), liver (L), and platelet (P; same as fibroblast) subunits, which are variably expressed in different tissues.…”

“…Three structural loci code for muscle (M), liver (L), and platelet (P; same as fibroblast) subunits, which are variably expressed in different tissues. Random tetramerization of these subunits produces various isozymes, which are distinguishable from one another by ion-exchange chromatography (3,4) and subunitspecific antibodies (3)(4)(5)(6). The molecular basis of inherited erythrocyte PFK deficiency has been investigated only in a few cases (7)(8)(9)(10)(11)(12)(13).…”

Phosphofructokinase in rat lung during perinatal development: characterization of subunit composition and regulation by fructose 2,6-bisphosphate and glucose 1,6-bisphosphate

“…Three types of phosphofructokinase subunits are known: M (muscle), L (liver) and C, recently reported to be present in rabbit [8] and rat [9] brain. Type C is also known as type F (fibroblast) [10] or P (platelet) [11]. Hybridization of the three subunits is possible.…”

Extraction and Detection of mRNA from Horsehair

“…Phosphofructokinase, one of the housekeeping genes belonging to the glycolytic pathway [3,7,32], codes for three isozymes with tissue-specific expression: muscle, liver and platelet types [6]. PFKM is the predominant subunit of the skeletal muscle, heart, and brain, which have their metabolic energy requirements principally met by glycolysis [7,8,12]. PFKM expression has not been reported in the hair follicle; however, as this gene is indirectly related to hair follicle growth, we attempted to detect its mRNA in hair fiber.…”