Phosphofructokinase from bumblebee flight muscle. Molecular and catalytic properties and role of the enzyme in regulation of the fructose 6-phosphate/fructose 1,6-bisphosphate cycle.

Leite, Amanda Melissa Damião; Neto, José Abrahão; Leyton, Jaime F.; Crivellaro, O; El-Dorry, Hamza A.

doi:10.1016/s0021-9258(19)77867-3

Cited by 21 publications

(3 citation statements)

References 28 publications

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“…In our study, we identified a number of putatively selected genes, potentially linked to thermal regulation (Tables 3 , S3 ). For instance, one annotated gene ( pfk ) was related to glycolysis regulation, and more specifically to the phosphofructokinase/fructose‐l,6‐bisphosphatase‐mediated substrate cycle (Leite et al, 1988 ). In bumblebees, this substrate cycle is critical for flight muscle activity, and is dependent on environmental temperature (Leite et al, 1988 ).…”

Section: Discussionmentioning

confidence: 99%

“…For instance, one annotated gene ( pfk ) was related to glycolysis regulation, and more specifically to the phosphofructokinase/fructose‐l,6‐bisphosphatase‐mediated substrate cycle (Leite et al, 1988 ). In bumblebees, this substrate cycle is critical for flight muscle activity, and is dependent on environmental temperature (Leite et al, 1988 ). Interestingly, at the SNP located on pfk gene, New Hampshire and Maine samples show similar genotypes.…”

Section: Discussionmentioning

confidence: 99%

“…For example, one SNP (Scaffold 3_12741967; hereafter, the number after the underscore refers to the SNP position within the scaffold) that was detected as an outlier associated with longitude, TA B L E 2 Estimates of pairwise θ (Weir & Cockerham, 1984) between all pairs of populations (above the diagonal), with their associated p-values (below the diagonal), as estimated using GenoDive v3.04 (Meirmans, 2020). S3) annotated to ATP-dependent 6-phosphofructokinase (Pfk), which is involved in glycometabolism (Leite et al, 1988); at this SNP, New Hampshire and Maine had the same genotype in contrast to all other populations. Furthermore, three outlier SNPs (Scaffold 1_18492518; Scaffold 1_18495934; Scaffold 1_18401015) found in association with longitude, temperature, and precipitation were located on Scaffold 1 and annotated to steroid receptor seven-up (svp) that has a role in eye development (Tsachaki & Sprecher, 2012), and king-tubby-a gene known for its function in retinal degeneration (Carbone et al, 2016).…”

Section: Outlier Detection and Annotation Of Outlier Snpsmentioning

confidence: 99%

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The impact of geography and climate on the population structure and local adaptation in a wild bee

Samad-zada

Kelemen

Rehan

2023

Evolutionary Applications

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Deciphering processes that contribute to genetic differentiation and divergent selection of natural populations is useful for evaluating the adaptive potential and resilience of organisms faced with various anthropogenic stressors. Insect pollinator species, including wild bees, provide critical ecosystem services but are highly susceptible to biodiversity declines. Here, we use population genomics to infer the genetic structure and test for evidence of local adaptation in an economically important native pollinator, the small carpenter bee (Ceratina calcarata). Using genome‐wide SNP data (n = 8302), collected from specimens across the species' entire distribution, we evaluated population differentiation and genetic diversity and identified putative signatures of selection in the context of geographic and environmental variation. Results of the analyses of principal component and Bayesian clustering were concordant with the presence of two to three genetic clusters, associated with landscape features and inferred phylogeography of the species. All populations examined in our study demonstrated a heterozygote deficit, along with significant levels of inbreeding. We identified 250 robust outlier SNPs, corresponding to 85 annotated genes with known functional relevance to thermoregulation, photoperiod, and responses to various abiotic and biotic stressors. Taken together, these data provide evidence for local adaptation in a wild bee and highlight genetic responses of native pollinators to landscape and climate features.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%