Phosphofructokinase-1 Negatively Regulates Neurogenesis from Neural Stem Cells

Zhang, Fengyun; Qian, Xiaodan; Cheng, Qi; Yang, Lin; Wu, Hai‐Yin; Chang, Lei; Luo, Chunxia; Zhu, Dong‐Ya

doi:10.1007/s12264-016-0032-y

Cited by 14 publications

(11 citation statements)

References 47 publications

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“…Cell culture. The primary mouse hippocampal neuron culture was performed by using previously described methods (21,22). Briefly, neonatal mice (0 days) were decapitated following anesthesia with isoflurane, and the hippocampi were carefully separated.…”

Section: Reverse Transcription-quantitative Polymerase Chain Reactionmentioning

confidence: 99%

MicroRNA‑449a regulates the progression of brain aging by targeting SCN2B in SAMP8 mice

et al. 2020

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Our previous study demonstrated that the expression of sodium channel voltage-gated beta 2 (ScN2B) increased with aging in senescence-accelerated mouse prone 8 (SAMP8) mice, and was identified to be associated with a decline in learning and memory, while the underlying mechanism is unclear. In the present study, multiple differentially expressed miRNAs, which may be involved in the process of aging by regulating target genes, were identified in the prefrontal cortex and hippocampus of SAMP8 mice though miRNA microarray analysis. Using bioinformatics prediction, ScN2B was identified to be one of the potential target genes of miR-449a, which was downregulated in the hippocampus. Previous studies demonstrated that miR-449a is involved in the occurrence and progression of aging by regulating a variety of target genes. Therefore, it was hypothesized that miR-449a may be involved in the process of brain aging by targeting ScN2B. To verify this hypothesis, the following experiments were conducted: A reverse transcription-quantitative polymerase chain reaction assay revealed that the expression level of miR-449a was significantly decreased in the prefrontal cortex and hippocampus of 12-month old SAMP8 mice; a dual-luciferase reporter assay verified that miR-449a regulated ScN2B expression by binding to the 3'-UTR 'seed region'; an anti-Ago co-immunoprecipitation combined with Affymetrix microarray analyses demonstrated that the target mRNA highly enriched with Ago-miRNPs was confirmed to be ScN2B. Finally, overexpression of miR-449a or inhibition of ScN2B promoted the extension of hippocampal neurons in vitro. The results of the present study suggested that miR-449a was downregulated in the prefrontal cortex and hippocampus of SAMP8 mice and may regulate the process of brain aging by targeting ScN2B.

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Section: Reverse Transcription-quantitative Polymerase Chain Reactionmentioning

confidence: 99%

MicroRNA‑449a regulates the progression of brain aging by targeting SCN2B in SAMP8 mice

et al. 2020

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“…PFKM is one of the isozymes encoded by phosphofructokinase-1 (PFK-1), which catalyzes the second irreversible step in the glycolytic pathway by phosphorylating fructose-6-phosphate to form fructose-1, 6-bisphosphate (45,46). It is the key regulatory enzyme mediating changes in glycolysis in cancer cells (45,46). However, the importance of PFKM and its upstream regulators in tumorigenic progression remains largely unknown.…”

Section: Discussionmentioning

confidence: 99%

Splice variant of growth hormone-releasing hormone receptor drives esophageal squamous cell carcinoma conferring a therapeutic target

Xiong

Wang

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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The extrahypothalamic growth hormone-releasing hormone (GHRH) and its cognate receptors (GHRH-Rs) and splice variants are expressed in a variety of cancers. It has been shown that the pituitary type of GHRH-R (pGHRH-R) mediates the inhibition of tumor growth induced by GHRH-R antagonists. However, GHRH-R antagonists can also suppress some cancers that do not express pGHRH-R, yet the underlying mechanisms have not been determined. Here, using human esophageal squamous cell carcinoma (ESCC) as a model, we were able to reveal that SV1, a known splice variant of GHRH-R, is responsible for the inhibition induced by GHRH-R antagonist MIA-602. We demonstrated that GHRH-R splice variant 1 (SV1) is a hypoxia-driven promoter of tumor progression. Hypoxia-elevated SV1 activates a key glycolytic enzyme, muscle-type phosphofructokinase (PFKM), through the nuclear factor kappa B (NF-κB) pathway, which enhances glycolytic metabolism and promotes progression of ESCC. The malignant actions induced by the SV1–NF-κB–PFKM pathway could be reversed by MIA-602. Altogether, our studies demonstrate a mechanism by which GHRH-R antagonists target SV1. Our findings suggest that SV1 is a hypoxia-induced oncogenic promoter which can be an alternative target of GHRH-R antagonists.

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“…Previous study showed that PFK-1 is regulated by signals from cell proliferation [ 50 , 51 ]. In addition, PFK-1 regulates the muscle fatigue by interaction with neuronal nitric oxide synthase [ 52 ]. PFK-1 and GAPDH play important roles in the increasing of muscle fibers size and the myoblast fusion in Drosophila [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome profiling of claw muscle of the mud crab (Scylla paramamosain) at different fattening stages

et al. 2017

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In crustaceans, muscle growth and development is complicated, and to date substantial knowledge gaps exist. In this study, the claw muscle, hepatopancreas and nervous tissue of the mud crab (Scylla paramamosain) were collected at three fattening stages for sequence by the Illumina sequencing. A total of 127.87 Gb clean data with no less than 3.94 Gb generated for each sample and the cycleQ30 percentages were more than 86.13% for all samples. De Bruijn assembly of these clean data produced 94,853 unigenes, thereinto, 50,059 unigenes were found in claw muscle. A total of 121 differentially expressed genes (DEGs) were revealed in claw muscle from the three fattening stages with a Padj value < 0.01, including 63 genes with annotation. Functional annotation and enrichment analysis showed that the DEGs clusters represented the predominant gene catalog with roles in biochemical processes (glycolysis, phosphorylation and regulation of transcription), molecular function (ATP binding, 6-phosphofructokinase activity, and sequence-specific DNA binding) and cellular component (6-phosphofructokinase complex, plasma membrane, and integral component of membrane). qRT-PCR was employed to further validate certain DEGs. Single nucleotide polymorphism (SNP) analysis obtained 159,322, 125,963 and 166,279 potential SNPs from the muscle transcriptome at stage B, stage C and stage D, respectively. In addition, there were sixteen neuropeptide transcripts being predicted in the claw muscle. The present study provides a comprehensive transcriptome of claw muscle of S. paramamosain during fattening, providing a basis for screening the functional genes that may affect muscle growth of S. paramamosain.

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Phosphofructokinase-1 Negatively Regulates Neurogenesis from Neural Stem Cells

Cited by 14 publications

References 47 publications

MicroRNA‑449a regulates the progression of brain aging by targeting SCN2B in SAMP8 mice

MicroRNA‑449a regulates the progression of brain aging by targeting SCN2B in SAMP8 mice

Splice variant of growth hormone-releasing hormone receptor drives esophageal squamous cell carcinoma conferring a therapeutic target

Transcriptome profiling of claw muscle of the mud crab (Scylla paramamosain) at different fattening stages

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