Phosphoethanolamine Enhances High‐Affinity Choline Uptake and Acetylcholine Synthesis in Dissociated Cell Cultures of the Rat Septal Nucleus

Bostwick, J. Robert; Abbe, Russell; Appel, Stanley H.

doi:10.1111/j.1471-4159.1992.tb08896.x

Cited by 10 publications

(6 citation statements)

References 26 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To shed light on the putative mechanisms involved in GPE-mediated effects, PL and ACh levels were measured. GPE has been tested in rat astrocytes and rat septal nucleus; however, we are the first to report on the effects of GPE on PL content in human NSCs.…”

Section: Results and Discussionmentioning

confidence: 98%

Human Neural Stem Cell Aging Is Counteracted by α-Glycerylphosphorylethanolamine

Daniele

Pozzo

Iofrida

et al. 2016

ACS Chem. Neurosci.

View full text Add to dashboard Cite

Neural stem cells (NSCs) represent a subpopulation of cells, located in specific regions of the adult mammalian brain, with the ability of self-renewing and generating neurons and glia. In aged NSCs, modifications in the amount and composition of membrane proteins/lipids, which lead to a reduction in membrane fluidity and cholinergic activities, have been reported. In this respect, molecules that are effective at normalizing the membrane composition and cholinergic signaling could counteract stem cell aging. α-Glycerylphosphorylethanolamine (GPE), a nootropic drug, plays a role in phospholipid biosynthesis and acetylcholine release. Herein, GPE was assayed on human NSC cultures and on hydroxyurea-aged cells. Using cell counting, colorimetric, and fluorimetric analyses, immunoenzymatic assays, and real time PCR experiments, NSC culture proliferation, senescence, reactive oxygen species, and ADP/ATP levels were assessed. Aged NSCs exhibited cellular senescence, decreased proliferation, and an impairment in mitochondrial metabolism. These changes included a substantial induction in the nuclear factor NF-κB, a key inflammatory mediator. GPE cell treatment significantly protected the redox state and functional integrity of mitochondria, and counteracted senescence and NF-κB activation. In conclusion, our data show the beneficial properties of GPE in this model of stem cell aging.

show abstract

Section: Results and Discussionmentioning

confidence: 98%

Human Neural Stem Cell Aging Is Counteracted by α-Glycerylphosphorylethanolamine

Daniele

Pozzo

Iofrida

et al. 2016

ACS Chem. Neurosci.

View full text Add to dashboard Cite

show abstract

“…Consistently, the administration of drugs able to restore membrane composition and functionality can ameliorate memory performances [14]. Among these compounds, GPE has emerged in preserving the well-being of astrocytes and neural stem cells [1,4,[15][16][17]. Herein, the effects of GPE on membrane and cellular well-being were verified in adult human hippocampal cells.…”

Section: Discussionmentioning

confidence: 99%

“…Finally, lifespan of yeast and human-derived cell cultures has been prolonged by phosphatidylethanolamine (PE) and its precursor ethanolamine [1,15]. Among biological precursor membrane constituents, alpha-glycerylphosphorylethanolamine (GPE) has been emerging as a promising PE and PC donor with the ability to promote Ach synthesis [1,16,17]. GPE has been proven to possess protective effects in amyloid-injured astrocytes [16] and in an aging model of human neural stem cells [1].…”

Section: Introductionmentioning

confidence: 99%

The Nootropic Drug Α-Glyceryl-Phosphoryl-Ethanolamine Exerts Neuroprotective Effects in Human Hippocampal Cells

Daniele

Mangano

Durando

et al. 2020

IJMS

View full text Add to dashboard Cite

Brain aging involves changes in the lipid membrane composition that lead to a decrease in membrane excitability and neurotransmitter release. These membrane modifications have been identified as contributing factors in age-related memory decline. In this sense, precursors of phospholipids (PLs) can restore the physiological composition of cellular membranes and produce valuable therapeutic effects in brain aging. Among promising drugs, alpha-glycerylphosphorylethanolamine (GPE) has demonstrated protective effects in amyloid-injured astrocytes and in an aging model of human neural stem cells. However, the compound properties on mature neuronal cells remain unexplored. Herein, GPE was tested in human hippocampal neurons, which are involved in learning and memory, and characterized by a functional cholinergic transmission, thus representing a valuable cellular model to explore the beneficial properties of GPE. GPE induced the release of the main membrane phospholipids and of the acetylcholine neurotransmitter. Moreover, the compound reduced lipid peroxidation and enhanced membrane fluidity of human brain cells. GPE counteracted the DNA damage and viability decrease observed in in vitro aged neurons. Among GPE treatment effects, the autophagy was found positively upregulated. Overall, these results confirm the beneficial effects of GPE treatment and suggest the compound as a promising drug to preserve hippocampal neurons and virtually memory performances.

show abstract

“…It is known that in the mammalian brain, PEA affects synthesis of acetylcholine in cholinergic neurons [25], induces apoptosis [26], and increases calcium activity [27]. Apparently, it is under low environmental temperatures stimulating the appearance and accumulation of PEA in the brain of ectothermic animals that there occurred formation of the above-mentioned functions.…”

Section: Discussionmentioning

confidence: 99%

Phosphoethanolamine in brain of the euritherm lake fish Perccottus glehni (Eleotridae, Perciformes, Dyb. 1877) as a phenomenon depending on temperature factor

Каранова

2013

J Evol Biochem Phys

View full text Add to dashboard Cite

The present work studied effect of a seasonal decrease of environmental temperature and cold shock on pools of free amino acids (FA) in brain of the euritherm lake fish P. glehni. For the first time, non-protein amino acid, phosphoethanolamine (PEA), revealed in the great amounts under conditions of near-zero temperatures was found in brain of ectothermic animals. An intensive rise of the PEA pool from 0.3% during the summer period to 33.6% at winter was shown to occur in the brain of P. glehni as a result of a seasonal temperature decrease. In contrast, the level of taurine showing the greatest pool in comparison with other FA (29.0% of the total pool) in summer decreases to 8.9% in the beginning of winter period. Similar negative correlation between the taurine and PEA content was found under effect of acute cold shock (1°C): the taurine level decreased for 4 days from 32.2 to 14.5% of the total pool, whereas the PEA level sharply increased (from 2.1 to 15.3%). Both types of the low-temperature actions have revealed, besides PEA, an intensive rise in the pool of serine and accumulation of phosphoserine. The role of PEA in biochemical evolution and adaptation of the brain to low temperatures is discussed. It is supposed that accumulation of PEA, phosphoserine, and serine is associated with changes in the status of membrane phospholipids at low temperatures.

show abstract

Phosphoethanolamine Enhances High‐Affinity Choline Uptake and Acetylcholine Synthesis in Dissociated Cell Cultures of the Rat Septal Nucleus

Cited by 10 publications

References 26 publications

Human Neural Stem Cell Aging Is Counteracted by α-Glycerylphosphorylethanolamine

Human Neural Stem Cell Aging Is Counteracted by α-Glycerylphosphorylethanolamine

The Nootropic Drug Α-Glyceryl-Phosphoryl-Ethanolamine Exerts Neuroprotective Effects in Human Hippocampal Cells

Phosphoethanolamine in brain of the euritherm lake fish Perccottus glehni (Eleotridae, Perciformes, Dyb. 1877) as a phenomenon depending on temperature factor

Contact Info

Product

Resources

About