Phosphoenolpyruvate carboxykinase, pyruvate orthophosphate dikinase and isocitrate lyase in both tomato fruits and leaves, and in the flesh of peach and some other fruits

Famiani, Franco; Paoletti, Andrea; Battistelli, Alberto; Moscatello, Stefano; Chen, Zhihui; Leegood, Richard C.; Walker, Robert P.

doi:10.1016/j.jplph.2016.07.003

Cited by 19 publications

(11 citation statements)

References 66 publications

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“…PPDK protein was not detected in the flesh of a range of soft fruits, plum, cherry or grape [ 22 , 41 , 64 , 65 ]. PPDK protein was detected in tomato flesh, and in this its abundance corresponded to an estimated activity of c0.003–0.014 µmol min −1 g −1 FW [ 12 ]. PEPCK activity in tomato, grape and cherry flesh is c0.1–0.3 µmol min −1 g −1 FW [ 16 , 64 , 66 ].…”

Section: Potential Occurrence Of Gluconeogenesismentioning

confidence: 99%

“…PPDK polypeptide has been detected in peach flesh [ 67 , 68 ]. However, throughout the ripening of peach flesh the amount of PPDK polypeptide is very low and its estimated activity is around 0.0003–0.0014 µmol min −1 g −1 FW [ 12 ]. By contrast PEPCK is quite abundant in peach flesh throughout ripening (c0.18 U g −1 FW), and this suggests that the bulk of any gluconeogenic flux utilises PEPCK [ 12 ].…”

Section: Potential Occurrence Of Gluconeogenesismentioning

confidence: 99%

“…However, throughout the ripening of peach flesh the amount of PPDK polypeptide is very low and its estimated activity is around 0.0003–0.0014 µmol min −1 g −1 FW [ 12 ]. By contrast PEPCK is quite abundant in peach flesh throughout ripening (c0.18 U g −1 FW), and this suggests that the bulk of any gluconeogenic flux utilises PEPCK [ 12 ]. Similarly, if PPDK is present in grape pericarp under normal conditions of growth it is likely to be at very low abundance compared to PEPCK [ 52 ].…”

Section: Potential Occurrence Of Gluconeogenesismentioning

confidence: 99%

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Gluconeogenesis in Plants: A Key Interface between Organic Acid/Amino Acid/Lipid and Sugar Metabolism

Walker¹,

Chen

Famiani

2021

Molecules

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The aims of this article are four-fold. First, to provide a concise overview of plant gluconeogenesis. Second, to emphasise the widespread occurrence of gluconeogenesis and its utilisation in diverse processes. Third, to stress the importance of the vacuolar storage and release of Krebs cycle acids/nitrogenous compounds; and of the role of gluconeogenesis and malic enzyme in this process. Fourth, to outline the contribution of fine control of enzyme activity to the coordinate-regulation of gluconeogenesis and malate metabolism; and the importance of cytosolic pH in this.

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Section: Potential Occurrence Of Gluconeogenesismentioning

confidence: 99%

Section: Potential Occurrence Of Gluconeogenesismentioning

confidence: 99%

Section: Potential Occurrence Of Gluconeogenesismentioning

confidence: 99%

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Gluconeogenesis in Plants: A Key Interface between Organic Acid/Amino Acid/Lipid and Sugar Metabolism

Walker¹,

Chen

Famiani

2021

Molecules

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“…During the accumulation of zein protein in developing endosperm, cyPPDKZm1 can be regulated by the O 2 ( Opaque2 ) gene [20]. In most C 3 tissues, PPDK is either not present or at very low abundance [21]. In rice, there are only two genes encoding PPDK, one encoding OsPPDKA and another producing two transcripts of C 4 -type chloroplastic PPDK (chOsPPDKB) and cytosolic PPDK (cyOsPPDKB) [22,23].…”

Section: Introductionmentioning

confidence: 99%

A Novel Mutation of OsPPDKB, Encoding Pyruvate Orthophosphate Dikinase, Affects Metabolism and Structure of Starch in the Rice Endosperm

Zhang

Zhao

Lin

et al. 2018

IJMS

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Starch, as a main energy storage substance, plays an important role in plant growth and human life. Despite the fact that several enzymes and regulators involved in starch biosynthesis have been identified, the regulating mechanism of starch synthesis is still unclear. In this study, we isolated a rice floury endosperm mutant M14 from a mutant pool induced by 60Co. Both total starch content and amylose content in M14 seeds significantly decreased, and starch thermal and pasting properties changed. Compound starch granules were defected in the floury endosperm of M14 seeds. Map-based cloning and a complementation test showed that the floury endosperm phenotype was determined by a gene of OsPPDKB, which encodes pyruvate orthophosphate dikinase (PPDK, EC 2.7.9.1). Subcellular localization analysis demonstrated that PPDK was localized in chloroplast and cytoplasm, the chOsPPDKB highly expressed in leaf and leaf sheath, and the cyOsPPDKB constitutively expressed with a high expression in developing endosperm. Moreover, the expression of starch synthesis-related genes was also obviously altered in M14 developing endosperm. The above results indicated that PPDK played an important role in starch metabolism and structure in rice endosperm.

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“…Two PPDK proteins with different sizes are produced and localized in chloroplast and cytosol; these proteins are the chloroplastic PPDK (chlPPDK) and cytosolic PPDK (cyPPDK), respectively (Chastain, 2010). Expression of cyPPDK is commonly considerably low (Aoyagi and Bassham, 1984;Chastain, 2010), except for in seeds (Kang et al, 2005;Manicacci et al, 2009), fruits (Famiani et al, 2016), or middle veins (Hibberd and Quick, 2002) of leaves in several plant species.…”

mentioning

confidence: 99%

Functional Characterization of Cytosolic Pyruvate Phosphate Dikinase Gene (MecyPPDK) and Promoter (MecyPPDKP) of Cassava in Response to Abiotic Stress in Transgenic Tobacco

Wang

Liu

et al. 2018

Crop Science

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Pyruvate phosphate dikinase (PPDK) is a key enzyme in the photosynthesis of C4 plants; its expression significantly affects the accumulation of photosynthetic products in C4 plants. Pyruvate phosphate dikinase presents two subtypes, namely, chloroplastic PPDK and cytosolic PPDK. Cytosolic PPDK is ubiquitous in C3 and C4 plants. This subtype is also highly expressed in metabolically active storage organs. Cytosolic PPDK in cassava (Manihot esculenta Crantz, MecyPPDK) is considerately expressed mainly in functional leaves. However, research on the function of PPDK in cassava has not yet been reported. In this study, we cloned the 5′ flanking sequence of MecyPPDK fused to the uidA reporter gene and stably transfected tobacco (Nicotiana benthamiana Domin). Slight β‐glucuronidase activity was observed in transformed tobacco. This activity was upregulated remarkably under low temperature, drought, waterlogging, and abscisic acid treatments. Results suggest that MecyPPDK facilitates the survival of cassava plants in response to abiotic stress. Our results will provide further understanding regarding the regulation of MecyPPDK expression and a novel stress‐inducible promoter in transgenic tobacco.

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Phosphoenolpyruvate carboxykinase, pyruvate orthophosphate dikinase and isocitrate lyase in both tomato fruits and leaves, and in the flesh of peach and some other fruits

Cited by 19 publications

References 66 publications

Gluconeogenesis in Plants: A Key Interface between Organic Acid/Amino Acid/Lipid and Sugar Metabolism

Gluconeogenesis in Plants: A Key Interface between Organic Acid/Amino Acid/Lipid and Sugar Metabolism

A Novel Mutation of OsPPDKB, Encoding Pyruvate Orthophosphate Dikinase, Affects Metabolism and Structure of Starch in the Rice Endosperm

Functional Characterization of Cytosolic Pyruvate Phosphate Dikinase Gene (MecyPPDK) and Promoter (MecyPPDKP) of Cassava in Response to Abiotic Stress in Transgenic Tobacco

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