Phosphatidylinositol-dependent bond between alkaline phosphatase and collagen fibers in the periodontal ligament of rat molars

Nakamura, Yoshiki; Noda, Koji; Shimpo, Satoshi; Oikawa, Takashi; Kawasaki, Kenzo; Hirashita, Ayao

doi:10.1007/s00418-003-0599-z

Cited by 10 publications

(5 citation statements)

References 21 publications

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“…This might be due to the activation of MAP kinase by CXCL12 [52], [53], which induces up-regulation of COL1A1 [54]–[56] and ALP [57], [58]. In addition, COL1A1 and ALP are requisite factors of PDL fibroblasts [59]. Collagen type I is a primary component of periodontal fibers and ALP is located in collagen fibers and fibrils, which are different characters from other connective tissues.…”

Section: Discussionmentioning

confidence: 99%

Function of Chemokine (CXC Motif) Ligand 12 in Periodontal Ligament Fibroblasts

et al. 2014

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The periodontal ligament (PDL) is one of the connective tissues located between the tooth and bone. It is characterized by rapid turnover. Periodontal ligament fibroblasts (PDLFs) play major roles in the rapid turnover of the PDL. Microarray analysis of human PDLFs (HPDLFs) and human dermal fibroblasts (HDFs) demonstrated markedly high expression of chemokine (CXC motif) ligand 12 (CXCL12) in the HPDLFs. CXCL12 plays an important role in the migration of mesenchymal stem cells (MSCs). The function of CXCL12 in the periodontal ligament was investigated in HPDLFs. Expression of CXCL12 in HPDLFs and HDFs was examined by RT-PCR, qRT-PCR and ELISA. Chemotactic ability of CXCL12 was evaluated in both PDLFs and HDFs by migration assay of MSCs. CXCL12 was also immunohistochemically examined in the PDL in vivo. Expression of CXCL12 in the HPDLFs was much higher than that in HDFs in vitro. Migration assay demonstrated that the number of migrated MSCs by HPDLFs was significantly higher than that by HDFs. In addition, the migrated MSCs also expressed CXCL12 and several genes that are familiar to fibroblasts. CXCL12 was immunohistochemically localized in the fibroblasts in the PDL of rat molars. The results suggest that PDLFs synthesize and secrete CXCL12 protein and that CXCL12 induces migration of MSCs in the PDL in order to maintain rapid turnover of the PDL.

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Section: Discussionmentioning

confidence: 99%

Function of Chemokine (CXC Motif) Ligand 12 in Periodontal Ligament Fibroblasts

et al. 2014

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“…An important physiological function of ALP is to catalyze the bone generation and mineralization. ALP activity increases during the development of bone formation (Coleman ; Nakamura, Noda, Shimpo, Oikawa, Kawasaki & Hirashita ). In this study, the plasma ALP activity of the soft‐shelled turtles showed the exact patterns as the plasma ALP activity reported in tilapia (Lin et al .…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary magnesium on the growth, carapace strength and tissue magnesium concentrations of soft-shelled turtle,Pelodiscus sinensis(Wiegmann)

Chen

Huang

2013

Aquac Res

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A feeding trial was conducted to evaluate the effects of dietary magnesium on the growth, carapace strength, tissue and serum Mg concentration of soft‐shelled turtles, Pelodiscus sinensis (Wiegmann). Juvenile soft‐shelled turtles of approximate 5.4 g body weight were fed diets with seven levels of Mg (48, 206, 369, 670, 955, 1195 and 1500 mg Mg kg−1) for eight weeks. No significant difference (P ≥ 0.05) was found in weight gain (WG), feed conversion ratio or protein efficiency ratio among treatments. However, the WG of turtles continued to increase with increasing dietary Mg levels up to 670 mg kg−1, beyond which the WG levelled off. The plasma alkaline phosphatase activity and the muscle, bone Mg concentrations of the turtles increased with the increasing dietary Mg levels between 48 and 955 mg kg−1, beyond which the tissue Mg concentrations remained relatively constant. Furthermore, the carapace strengths of turtles fed with the control diet of 48 mg Mg kg−1 were significantly weaker (P < 0.05) than that of turtles fed with diets containing higher Mg levels. Based on a broken‐line modelling analysis, the required dietary Mg level for the optimal WG of juvenile soft‐shelled turtles was estimated to be approximately 650 mg kg−1. By contrast, the required dietary Mg levels for turtles to reach the optimal muscle and bone Mg concentrations were 1050 and 1000 mg kg−1 respectively. The required dietary Mg level for maximal alkaline phosphatase activity was approximately 980 mg kg−1.

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“…Critical for successful immunolocalization was the use of acetic acid for demineralization of the methanol-fixed tissue. A throughout controlled study in regard to fixation and incubation conditions revealed alkaline phosphatase not only at membranes of osteoblasts and fibroblasts of the periodontal ligament but additionally on collagen fibers of the extracellular matrix (Nakamura et al 2004).…”

Section: New Methods and Toolsmentioning

confidence: 99%

Recent progress in histochemistry and cell biology: the state of the art 2005

Taatjes

Roth

2005

Histochem Cell Biol

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Advances in the field of histochemistry, a multidisciplinary area including the detection, localization and functional characterization of molecules in single cells and complex tissues, often drives the attainment of new knowledge in the broadly defined discipline of cell biology. These two disciplines, histochemistry and cell biology, have been joined in this journal to facilitate the flow of information with celerity from technical advancement in histochemical procedures, to their utilization in experimental models. This review summarizes advancements in these fields during the past year.

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Phosphatidylinositol-dependent bond between alkaline phosphatase and collagen fibers in the periodontal ligament of rat molars

Cited by 10 publications

References 21 publications

Function of Chemokine (CXC Motif) Ligand 12 in Periodontal Ligament Fibroblasts

Function of Chemokine (CXC Motif) Ligand 12 in Periodontal Ligament Fibroblasts

Effects of dietary magnesium on the growth, carapace strength and tissue magnesium concentrations of soft-shelled turtle,Pelodiscus sinensis(Wiegmann)

Recent progress in histochemistry and cell biology: the state of the art 2005

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