Phosphatidylinositol 3‐Kinase–DNA Methyltransferase 1–miR‐1281–Histone Deacetylase 4 Regulatory Axis Mediates Platelet‐Derived Growth Factor–Induced Proliferation and Migration of Pulmonary Artery Smooth Muscle Cells

Li, Yanjiao; Li, Li; Qian, Zhengjiang; Lin, Boya; Chen, Jidong; Luo, Yixuan; Qu, Junle; Raj, J. Usha; Gou, Deming

doi:10.1161/jaha.117.007572

Cited by 28 publications

(15 citation statements)

References 86 publications

(140 reference statements)

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“…Apart from the histone deacetylase 4 (HDAC4) gene, that has been recently shown as a direct target of miR-1281 in pulmonary artery smooth muscle cells (54), no other targets of miR-1281 have been identified yet. Our in vitro results from human ARPE-19 retinal cells show that the extracellular release of miR-1281 following high glucose treatment significantly exceeded the release under low glucose conditions, and this behavior only partially reflected that of endothelial HUVEC cells, in which miR-1281 was also significantly reduced in the intracellular environment, although to a lesser extent than in ARPE-19 cells, but was unchanged in the extracellular medium.…”

Section: Discussionmentioning

confidence: 99%

“…Until now, only few studies have been published in which authors had identified miR-1281 as a potential biomarker in human diseases. Among these disease states were abdominal aortic aneurysm ( 53 ), coronary heart disease and pulmonary arterial hypertension ( 54 ), chronic nephropathy ( 55 ), immune thrombocytopenic purpura ( 56 ), and malignant pleural mesothelioma ( 57 ). Our findings in the present work are consistent with these studies, implicating miR-1281 as a potential biomarker to track disease progression or aid in disease diagnosis.…”

Section: Discussionmentioning

confidence: 99%

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MicroRNA-1281 as a Novel Circulating Biomarker in Patients With Diabetic Retinopathy

et al. 2020

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Objective: Recently, the role of circulating miRNAs as non-invasive biomarkers for the identification and monitoring of diabetes microvascular complications has emerged. Herein, we aimed to: identify circulating miRNAs differentially expressed in patients with and without diabetic retinopathy (DR); examine their predictive value; and understand their pathogenic impact. Methods: Pooled serum samples from randomly selected matched patients with type 2 diabetes, either with or without DR, were used for initial serum miRNA profiling. Validation of the most relevant miRNAs was thereafter conducted by RT-qPCR in an extended sample of patients with DR and matched controls. Results: Following miRNA profiling, 43 miRNAs were significantly up-or down-regulated in patients with DR compared with controls. After individual validation, 5 miRNAs were found significantly overexpressed in patients with DR. One of them, miR-1281, was the most up-regulated and appeared to be specifically related to DR. Furthermore, secreted levels of miR-1281 were increased in high glucose-cultured retinal cells, and there was evidence of a potential link between glucose-induced miR-1281 up-regulation and DR. Conclusion: Our findings suggest miR-1281 as a circulating biomarker of DR. Also, they highlight the pathogenic significance of miR-1281, providing insights for a new potential target in treating DR.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

MicroRNA-1281 as a Novel Circulating Biomarker in Patients With Diabetic Retinopathy

et al. 2020

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“…2C]. MiRNA-1281 suppresses proliferation and migration in pulmonary artery smooth muscle cells, likely through targeting histone deacetylase 4 (HDAC4) (Li et al 2018b). In osteosarcoma, miR-1281…”

Section: Discussion____________________________________________________mentioning

confidence: 99%

Impaired miRNA degradation by post-transcriptional addition of 3’ cytosine and adenine in T cell activation

Rodríguez-Galán

Dosil

Gómez

et al. 2020

Preprint

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MiRNA repertoire of T cells undergoes extensive changes in response to activation. Whereas global miRNA downregulation occurs few hours after activation, some individual miRNAs are specifically up- or down-regulated. In this study, we have assessed miRNA expression and post-transcriptional modification kinetics in human primary CD4+ T cells upon short-term stimulation with anti-CD3/antiCD28 or IFN I using Next Generation Sequencing. Multiple miRNAs not related before with T cell activation profile have been identified as differentially expressed. Downregulated miRNAs presented higher 3' uridylation. Dis3L2 and Eri1 (3' to 5' exoribonucleases that prefer uridylated RNA as substrates) increased their expression upon TCR stimulation, probably generating an adverse environment for miRNAs. Remarkably, non-templated cytosine additions to 3' end, previously unknown to be a relevant post-transcriptional modification mechanism, were overrepresented in upregulated miRNAs, together with high levels of adenylation. In the midst of an increasing presence of exoribonucleases, miRNAs multiplying their levels may successfully escape degradation due to 3' cytosine and adenine addition. These protective signals open a new avenue to improve miRNA stability for therapy in T cells.

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“…All the obtained constructs were verified by DNA sequencing. The luciferase activities were detected as described previously [212223]. Chemically synthesized miR-181a mimic and the corresponding negative control (NC) were provided by Ruibobio (Guangzhou, China).…”

Section: Methodsmentioning

confidence: 99%

LINC00703 Acts as a Tumor Suppressor via Regulating miR-181a/KLF6 Axis in Gastric Cancer

Yang

Peng

et al. 2019

J Gastric Cancer

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Purpose: Long noncoding RNA 00703 (LINC00703) was found originating from a region downstream of Kruppel-like factor 6 (KLF6) gene, having 2 binding sites for miR-181a. Since KLF6 has been reported as a target of miR-181a in gastric cancer (GC), this study aims to investigate whether LINC00703 regulates the miR-181a/KLF6 axis and plays a functional role in GC pathogenesis. Materials and Methods: GC tissues, cell lines, and nude mice were included in this study. RNA binding protein immunoprecipitation (RIP) and pull-down assays were used to evaluate interaction between LINC00703 and miR-181a. Quantitative real-time polymerase chain reaction and western blot were applied for analysis of gene expression at the transcriptional and protein levels. A nude xenograft mouse model was used to determine LINC00703 function in vivo. Results: We revealed that LINC00703 competitively interacts with miR-181a to regulate KLF6. Overexpression of LINC00703 inhibited cell proliferation, migration/invasion, but promoted apoptosis in vitro, and arrested tumor growth in vivo. LINC00703 expression was found to be decreased in GC tissues, which was positively correlated with KLF6, but negatively with the miR-181a levels. Conclusions: LINC00703 may have an anti-cancer function via modulation of the miR-181a/ KLF6 axis. This study also provides a new potential diagnostic marker and therapeutic target for GC treatment.

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Phosphatidylinositol 3‐Kinase–DNA Methyltransferase 1–miR‐1281–Histone Deacetylase 4 Regulatory Axis Mediates Platelet‐Derived Growth Factor–Induced Proliferation and Migration of Pulmonary Artery Smooth Muscle Cells

Cited by 28 publications

References 86 publications

MicroRNA-1281 as a Novel Circulating Biomarker in Patients With Diabetic Retinopathy

MicroRNA-1281 as a Novel Circulating Biomarker in Patients With Diabetic Retinopathy

Impaired miRNA degradation by post-transcriptional addition of 3’ cytosine and adenine in T cell activation

LINC00703 Acts as a Tumor Suppressor via Regulating miR-181a/KLF6 Axis in Gastric Cancer

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