Phosphatidylglycerophosphate synthase from yeast

Minskoff, Stacey A.; Greenberg, Miriam L.

doi:10.1016/s0005-2760(97)00116-1

Cited by 21 publications

(9 citation statements)

References 18 publications

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“…Mitochondrial enzymes of CL and PG synthesis, as well as the major PS decarboxylase, are synthesized on cytosolic ribosomes, imported into mitochondria, and assembled into the IMM (Minskoff and Greenberg 1997;Jiang et al 2000;Nowicki et al 2005;Nebauer et al 2007;Choi et al 2012). Only recently were we able to demonstrate experimentally the involvement of components of the mitochondrial TOM complex and of mitochondrial proteases in import and processing of the mitochondrial PS decarboxylase Psd1p from the yeast (Horvath et al 2012).…”

Section: Major Phospholipid Classes Of Mitochondriamentioning

confidence: 99%

Lipid Transport between the Endoplasmic Reticulum and Mitochondria

Flis

Daum

2013

Cold Spring Harbor Perspectives in Biology

160

124

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Mitochondria are partially autonomous organelles that depend on the import of certain proteins and lipids to maintain cell survival and membrane formation. Although phosphatidylglycerol, cardiolipin, and phosphatidylethanolamine are synthesized by mitochondrial enzymes, phosphatidylcholine, phosphatidylinositol, phosphatidylserine, and sterols need to be imported from other organelles. The origin of most lipids imported into mitochondria is the endoplasmic reticulum, which requires interaction of these two subcellular compartments. Recently, protein complexes that are involved in membrane contact between endoplasmic reticulum and mitochondria were identified, but their role in lipid transport is still unclear. In the present review, we describe components involved in lipid translocation between the endoplasmic reticulum and mitochondria and discuss functional as well as regulatory aspects that are important for lipid homeostasis.

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Section: Major Phospholipid Classes Of Mitochondriamentioning

confidence: 99%

Lipid Transport between the Endoplasmic Reticulum and Mitochondria

Flis

Daum

2013

Cold Spring Harbor Perspectives in Biology

160

124

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“…First, phosphatidylglycerophosphate (PtdGro-P) is synthesized from CDP-DAG and glycerol-3-phosphate by the action of phosphatidylglycerophosphate synthase (for a review, see ref. 171). The PGS1 gene encoding this enzyme has only recently been identified.…”

Section: Phospholipid Synthesismentioning

confidence: 99%

Biochemistry, cell biology and molecular biology of lipids ofSaccharomyces cerevisiae

Daum

Lees

Bard

et al. 1998

Yeast

573

377

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The yeast Saccharomyces cerevisiae is a powerful experimental system to study biochemical, cell biological and molecular biological aspects of lipid synthesis. Most but not all genes encoding enzymes involved in fatty acid, phospholipid, sterol or sphingolipid biosynthesis of this unicellular eukaryote have been cloned, and many gene products have been functionally characterized. Less information is available about genes and gene products governing the transport of lipids between organelles and within membranes, turnover and degradation of complex lipids, regulation of lipid biosynthesis, and linkage of lipid metabolism to other cellular processes. Here we summarize current knowledge about lipid biosynthetic pathways in S. cerevisiae and describe the characteristic features of the gene products involved. We focus on recent discoveries in these fields and address questions on the regulation of lipid synthesis, subcellular localization of lipid biosynthetic steps, cross‐talk between organelles during lipid synthesis and subcellular distribution of lipids. Finally, we discuss distinct functions of certain key lipids and their possible roles in cellular processes. © 1998 John Wiley & Sons, Ltd.

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“…PGPSs from both prokaryotes and eukaryotes are integral membrane proteins. In yeast cells PGPS is exclusively located in the inner mitochondrial membrane, which provides the substrate for the formation of cardiolipin, the typical mitochondrial membrane lipid [5,7,8]. On the other hand, in plants PG synthesis has been demonstrated to occur in different subcellular compartments [9].…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, in plants PG synthesis has been demonstrated to occur in different subcellular compartments [9]. Apart from the mitochondrial pathway conserved in all eukaryotes [4,8–11], plants possess a plastidial pathway for PG synthesis, which is located in the inner envelope membranes and which is the dominant one for PG synthesis in leaves [12]. Furthermore, the endoplasmic reticulum of plants as well as of animal cells possesses all enzymic activities required for de novo PG synthesis [10,13].…”

Section: Introductionmentioning

confidence: 99%

Phosphatidylglycerophosphate synthases from Arabidopsis thaliana

Müller¹,

Frentzen²

2001

FEBS Letters

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Two Arabidopsis thaliana genes were shown to encode phosphatidylglycerophosphate synthases (PGPS) of 25.4 and 32.2 kDa, respectively. Apart from their N-terminal regions, the two proteins exhibit high sequence similarity. Functional expression studies in yeast provided evidence that the 25.4 kDa protein is a microsomal PGPS while the 32.2 kDa protein represents a preprotein which can be imported into yeast mitochondria and processed to a mature PGPS. The two isozymes were solubilized and purified as fusion proteins carrying a His tag at their C-terminus. Enzyme assays with both membrane fractions and purified enzyme fractions revealed that the two A. thaliana isozymes have similar properties but differ in their CDP-diacylglycerol species specificity. ß

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Phosphatidylglycerophosphate synthase from yeast

Cited by 21 publications

References 18 publications

Lipid Transport between the Endoplasmic Reticulum and Mitochondria

Lipid Transport between the Endoplasmic Reticulum and Mitochondria

Biochemistry, cell biology and molecular biology of lipids ofSaccharomyces cerevisiae

Phosphatidylglycerophosphate synthases from Arabidopsis thaliana

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