Phosphatidylglycerol Composition Is Central to Chilling Damage in the Arabidopsis <i>fab1</i> Mutant

Gao, Jinpeng; Lunn, Daniel; Wallis, James G.; Browse, John

doi:10.1104/pp.20.01219

Cited by 9 publications

(6 citation statements)

References 60 publications

(9 reference statements)

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“…Finally, 16:0-ACP is elongated by KASII to 18:0-ACP and then desaturated by SAD to generate 18:1-ACP (Lindqvist et al, 1996;Carlsson et al, 2002). The content of C16:0 in Arabidopsis KASII mutant (kas2) was remarkably increased (Gao et al, 2020), whereas the decreased activity of AAD5 resulted in a significant reduction in the level of C18:1 in mature seeds (Kazaz et al, 2020). Thus, the decrease of AtAAD5 expression led to the reduction of C18:1 in transgenic Arabidopsis, while the upregulation of PrSAD and PrKASII increased the C18:0 and C18:1 in PrASIL1-silenced tree peony.…”

Section: Discussionmentioning

confidence: 99%

A Tree Peony Trihelix Transcription Factor PrASIL1 Represses Seed Oil Accumulation

Yang

Behera

et al. 2021

Front. Plant Sci.

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In many higher plants, seed oil accumulation is governed by complex multilevel regulatory networks including transcriptional regulation, which primarily affects fatty acid biosynthesis. Tree peony (Paeonia rockii), a perennial deciduous shrub endemic to China is notable for its seed oil that is abundant in unsaturated fatty acids. We discovered that a tree peony trihelix transcription factor, PrASIL1, localized in the nucleus, is expressed predominantly in developing seeds during maturation. Ectopic overexpression of PrASIL1 in Nicotiana benthamiana leaf tissue and Arabidopsis thaliana seeds significantly reduced total fatty acids and altered the fatty acid composition. These changes were in turn associated with the decreased expression of multitudinous genes involved in plastidial fatty acid synthesis and oil accumulation. Thus, we inferred that PrASIL1 is a critical transcription factor that represses oil accumulation by down-regulating numerous key genes during seed oil biosynthesis. In contrary, up-regulation of oil biosynthesis genes and a significant increase in total lipids and several major fatty acids were observed in PrASIL1-silenced tree peony leaves. Together, these results provide insights into the role of trihelix transcription factor PrASIL1 in controlling seed oil accumulation. PrASIL1 can be targeted potentially for oil enhancement in tree peony and other crops through gene manipulation.

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Section: Discussionmentioning

confidence: 99%

A Tree Peony Trihelix Transcription Factor PrASIL1 Represses Seed Oil Accumulation

Yang

Behera

et al. 2021

Front. Plant Sci.

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“…KAS2, also known as fatty acid biosynthesis1 (fab1), has a degree of 3082 in this network. The fab1 mutant has increased levels of the saturated fatty acid 16:0, resulting in embryo arrest at the early embryo development stage [ 26 ]. Plastidic pyruvate kinase (PKPs) catalyze the synthesis of pyruvate and ATP; PKP1 and PKP2 have degrees of 3042 and 3038 in this network, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Meanwhile, 82 seed development or embryo development-related genes are enriched in our MElightgreen module. Previous studies have shown that some of these genes regulate seed development in Arabidopsis, including Fatty Acid Biosynthesis1 ( KAS2/FAB1 ) [ 26 ], plastidic pyruvate kinase PKP1 and PKP2 [ 27 ], NF-YA family member6 ( NFYA6 ) [ 45 ], carboxypeptidase ALTERED MERISTEM PROGRAM 1 ( AMP1 ) [ 46 ], β-Ketoacyl-[acyl carrier protein] synthase I ( KASI ) [ 44 ], and the homeobox genes MERISTEM LAYER1 ( ATML1 ) and PROTODERMAL FACTOR2 ( PDF2 ) [ 47 ]. These genes have many degrees in the MElightgreen module co-expression network ( Figure 6 D).…”

Section: Discussionmentioning

confidence: 99%

Knockdown NRPC2, 3, 8, NRPABC1 and NRPABC2 Affects RNAPIII Activity and Disrupts Seed Development in Arabidopsis

Zhao

Qin

Xiao

et al. 2021

IJMS

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RNA polymerase III (RNAPIII) contains 17 subunits forming 4 functional domains that control the different stages of RNAPIII transcription and are dedicated to the synthesis of small RNAs such as 5S rRNA and tRNAs. Here, we identified 23 genes encoding these subunits in Arabidopsis (Arabidopsis thaliana) and further analyzed 5 subunits (NRPC2, NRPC3, NRPC8, NRPABC1, and NRPABC2) encoded by 6 genes with different expression patterns and belonging to different sub-complexes. The knockdown of these genes repressed the expression of 5S rRNA and tRNAs, causing seed developmental arrest at different stages. Among these knockdown mutants, RNA-seq analysis revealed 821 common differentially expressed genes (DEGs), significantly enriched in response to stress, abscisic acid, cytokinins, and the jasmonic acid signaling pathway. Weighted gene co-expression network analysis (WGCNA) revealed several hub genes involved in embryo development, carbohydrate metabolic and lipid metabolic processes. We identified numerous unique DEGs between the mutants belonging to pathways, including cell proliferation, ribosome biogenesis, cell death, and tRNA metabolic processes. Thus, NRPC2, NRPC3, NRPC8, NRPABC1, and NRPABC2 control seed development in Arabidopsis by influencing RNAPIII activity and, thus, hormone signaling. Reduced expression of these subunit genes causes an insufficient accumulation of the total RNAPIII, leading to the phenotypes observed following the genetic knockdown of these subunits.

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“…The exact reasons for these marked effects are not clear, but it was shown that changes in the composition of membrane lipids, induced by modifications of different lipid biosynthesis genes, affect photosynthetic properties (Botté et al, 2011; Kobayashi, 2016; Gao et al, 2020). Thus, it seems conceivable that the altered chloroplast membrane composition observed in FAX1 overexpressors (Supplemental Figure 1 and Figure 8) is causative for the impaired photosynthetic performance.…”

Section: Discussionmentioning

confidence: 99%

Degradation of Fatty Acid Export Protein1 by Rhomboid-Like Protease11 Contributes to Cold Tolerance in Arabidopsis

John,

Krämer,

Lehmann

et al. 2023

Preprint

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Plants need to adapt to different stresses to optimize growth under unfavorable conditions. The abundance of the chloroplast envelope located Fatty Acid Export Protein1 (FAX1) decreases after the onset of low temperatures. However, it was unclear how FAX1 degradation occurs and whether altered FAX1 abundance contributes to cold tolerance in plants. The rapid cold-induced increase in rhomboid-like protease11 (RBL11) transcript, the physical interaction of RBL11 with FAX1, the specific FAX1 degradation after RBL11 expression, and the absence of cold-induced FAX1 degradation in rbl11 loss-of-function mutants suggest that this enzyme is responsible for FAX1 degradation. Proteomic analyses showed that rbl11 mutants have higher levels of FAX1 and other proteins involved in membrane lipid homeostasis, suggesting that RBL11 is a key element in the remodeling of membrane properties during cold. Consequently, in the cold, rbl11 mutants show a shift in lipid biosynthesis towards the eukaryotic pathway, which coincides with impaired cold tolerance. To demonstrate that cold sensitivity is due to increased FAX1 levels, FAX1 overexpressors were analyzed. rbl11 and FAX1 overexpressor mutants show superimposable phenotypic defects upon exposure to cold temperatures. Our results show that the cold-induced degradation of FAX1 by RBL11 is critical for Arabidopsis to survive cold and freezing periods.

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Phosphatidylglycerol Composition Is Central to Chilling Damage in the Arabidopsis fab1 Mutant

Abstract: Arabidopsis fab1 mutants die at 2°C; however, reducing levels of high-melting-point phosphatidylglycerol species through expression of a chloroplast-targeted 16:0 desaturase rescues fab1 plants.

Cited by 9 publications

References 60 publications

A Tree Peony Trihelix Transcription Factor PrASIL1 Represses Seed Oil Accumulation

A Tree Peony Trihelix Transcription Factor PrASIL1 Represses Seed Oil Accumulation

Knockdown NRPC2, 3, 8, NRPABC1 and NRPABC2 Affects RNAPIII Activity and Disrupts Seed Development in Arabidopsis

Degradation of Fatty Acid Export Protein1 by Rhomboid-Like Protease11 Contributes to Cold Tolerance in Arabidopsis

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