Phosphatidylethanol (Peth) in Dried Blood Spots: Development, validation and comparison between LC-MS/MS and QTOF methods

Ververi, Christina; Massano, Marta; Gerace, Enrico; Alladio, Eugenio; Vincenti, Marco; Salomone, Alberto

doi:10.1016/j.toxac.2022.06.065

Cited by 1 publication

(4 citation statements)

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“…Phosphatidylethanol (PEth) is a unique direct ethanol biomarker with a broad detection window to determine alcohol consumption or abstinence. PEth 16:0/18:1, the most prominent homologue in human blood, can act as a complement to the ethyl glucuronide, a biomarker in urine, to distinguish alcohol abusers from social drinkers 148 . In the year 2022, several research articles focused on the analysis of PEth 16:0/18:1 from microsamples acquired via DBS or VAMS technology.…”

Section: Applicationsmentioning

confidence: 99%

“…In the year 2022, several research articles focused on the analysis of PEth 16:0/18:1 from microsamples acquired via DBS or VAMS technology. Two analytical methods were developed to quantify PEth in DBS samples, where the detection of PEth was achieved using UHPLC‐QTOF‐HRMS, UHPLC‐MS/MS and Orbitrap‐based HRMS 148,149 . In a study quantifying DBS PEth using an LC‐MS/MS method, the threshold of PEth for excessive alcohol consumption was determined to be 160 ng/mL when the blood alcohol concentration decision limit was 50 mg/dL.…”

Section: Applicationsmentioning

confidence: 99%

“…PEth 16:0/18:1, the most prominent homologue in human blood, can act as a complement to the ethyl glucuronide, a biomarker in urine, to distinguish alcohol abusers from social drinkers. 148 In the year 2022, several research articles focused on the analysis of PEth 16:0/18:1 from microsamples acquired via DBS or VAMS technology. Two analytical methods were developed to quantify PEth in DBS samples, where the detection of PEth was achieved using UHPLC‐QTOF‐HRMS, UHPLC‐MS/MS and Orbitrap‐based HRMS.…”

Section: Applicationsmentioning

confidence: 99%

“…Two analytical methods were developed to quantify PEth in DBS samples, where the detection of PEth was achieved using UHPLC‐QTOF‐HRMS, UHPLC‐MS/MS and Orbitrap‐based HRMS. 148 , 149 In a study quantifying DBS PEth using an LC‐MS/MS method, the threshold of PEth for excessive alcohol consumption was determined to be 160 ng/mL when the blood alcohol concentration decision limit was 50 mg/dL. This threshold provided a sensitivity of 92% and a specificity of 90% for predicting excessive alcohol consumption.…”

Section: Applicationsmentioning

confidence: 99%

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Blood microsampling technologies: Innovations and applications in 2022

Thangavelu

Wouters

Kindt

et al. 2023

Analytical Science Advances

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With the development of highly sensitive bioanalytical techniques, the volume of samples necessary for accurate analysis has reduced. Microsampling, the process of obtaining small amounts of blood, has thus gained popularity as it offers minimal‐invasiveness, reduced logistical costs and biohazard risks while simultaneously showing increased sample stability and a potential for the decentralization of the approach and at‐home self‐sampling. Although the benefits of microsampling have been recognised, its adoption in clinical practice has been slow. Several microsampling technologies and devices are currently available and employed in research studies for various biomedical applications. This review provides an overview of the state‐of‐the‐art in microsampling technology with a focus on the latest developments and advancements in the field of microsampling. Research published in the year 2022, including studies (i) developing strategies for the quantitation of analytes in microsamples and (ii) bridging and comparing the interchangeability between matrices and choice of technology for a given application, is reviewed to assess the advantages, challenges and limitations of the current state of microsampling. Successful implementation of microsampling in routine clinical care requires continued efforts for standardization and harmonization. Microsampling has been shown to facilitate data‐rich studies and a patient‐centric approach to healthcare and is foreseen to play a central role in the future digital revolution of healthcare through continuous monitoring to improve the quality of life.

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Section: Applicationsmentioning

confidence: 99%

Section: Applicationsmentioning

confidence: 99%

Section: Applicationsmentioning

confidence: 99%

Section: Applicationsmentioning

confidence: 99%

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