Phosphatidic acid and diacylglycerol synergize in a cell-free system for activation of NADPH oxidase from human neutrophils.

Qualliotine-Mann, Diane; Agwu, David E.; Ellenburg, Mary D.; McCall, Charles E.; McPhail, Linda C.

doi:10.1016/s0021-9258(20)80462-1

Cited by 89 publications

(8 citation statements)

References 64 publications

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“…The synergistic relationship between DG and PS has been well characterized for activation of protein kinase C (PKC) (Bell & Burns, 1991;Newton, 1993). DG and PA act synergistically to stimulate NADPH oxidase (Qualliotine-Mann et al, 1993). In addition, previous work by Sleight and Dao (1990) demonstrated a synergistic activation of CT by phosphatidylethanolamine and oleic acid.…”

Section: Discussionmentioning

confidence: 99%

Lipid Regulation of CTP:Phosphocholine Cytidylyltransferase: Electrostatic, Hydrophobic, and Synergistic Interactions of Anionic Phospholipids and Diacylglycerol

1996

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The contributions of electrostatic and hydrophobic interactions in the activation of cytidylyl-transferase (CT) by various negatively charged lipids were analyzed using small unilamellar or multilamellar vesicles (SUVs or MLVs). The activation of CT by SUVs containing increasing mole percentages of anionic phospholipids varied in proportion to the net charge associated with the polar head group, suggesting an electrostatic component to the activation. However, increasing ionic strength to neutralize the surface charge enhanced the potency of SUVs containing PA or PG, suggesting that the hydrophobic effect is a stronger force than electrostatics in driving the interaction of CT with SUVs. On the other hand, electrostatics played a more important role in the activation by MLVs. Increasing ionic strength decreased the potency of MLVs containing PG. CT bound to MLVs in the gel state, but was inactive; the enzyme was only active when the MLVs were in the liquid-crystalline state, suggesting an intercalation event. Lowering the pH from 7.4 to 6.2 resulted in a decrease in the negative surface charge required for activation. The binding of CT to PG vesicles was enhanced at acidic pH. The results suggest that at pH 6.2 one or more amino acids on CT that are involved in lipid binding would be protonated. This could enhance the electrostatic effect by increasing the positive charge on CT, or it could enhance the hydrophobic effect by decreasing the negative charge on CT. In addition, maximal activity of CT was decreased at the lower pH, suggesting that active site residues may also be affected. CT was activated by the synergistic interaction of diacylglycerol and anionic phospholipid in SUVs. The synergy between DG and PA at low concentrations suggests the possibility that these second messenger lipids could concertedly regulate CT and thus PC synthesis in response to agonists that stimulate PC hydrolysis via phospholipases C and/or D.

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Section: Discussionmentioning

confidence: 99%

Lipid Regulation of CTP:Phosphocholine Cytidylyltransferase: Electrostatic, Hydrophobic, and Synergistic Interactions of Anionic Phospholipids and Diacylglycerol

1996

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“…Although it now seems certain that plant cells do not contain protein kinase C homologues (Harper et al, 1991;Dr~bak, 1992), plant cell transmembrane signaling appears to use all the components of the polyphosphoinositide signaling pathway (Dr~bak, 1992). Recently, however, a number of PKC-independent pathways have been identified for animal cells that also use both DAG and IP3 for signaling (Kramer et ai., 1987;Qualliotine-Mann et al, 1993;Roldan et ai., 1994;Rosenthal et al, 1993). In all instances, DAG was found to stimulate the activity of PLA2 leading to the production of the pharmacologically active arachidonic acid.…”

Section: Phospholipids and Phospholipid Metabolites As Mediators Of Cellular Signaling And Cytoskeletal Reorganizationmentioning

confidence: 99%

“…Our observation that phorbol esters (activators of PKC) have no effect on the tension in transvacuolar strands, while significant tension-relieving activity is observed for both 1,2 DiC8 and 1,3 DiC8 (diacylglycerol analogues) provides further evidence that the cytoskeletal changes must occur through a PKC-independent pathway that is mediated by DAG. The ability for both 1,2 and 1,3 DiC8 to mediate a biological phenomena has been demonstrated to be a characteristic element of pathways that use DAG to activate PLA2 (Resenthal et al, 1993;Roldan and Fragio, 1994;Qualliotine-Mann et al, 1993).…”

Section: Phospholipids and Phospholipid Metabolites As Mediators Of Cellular Signaling And Cytoskeletal Reorganizationmentioning

confidence: 99%

Lipids trigger changes in the elasticity of the cytoskeleton in plant cells: a cell optical displacement assay for live cell measurements.

Grabski

Xie

Holland

et al. 1994

The Journal of Cell Biology

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Abstract. An assay has been developed to quantitatively measure the tension and elasticity of the cytoskeleton in living plant cells. The cell optical displacement assay (CODA) uses a focused laser beam to optically trap and displace transvacuolar and cortical strands through a defined distance within the cell. Results from these experiments provide evidence for the classification of at least two rheologically distinct cytoskeletal assemblies, cortical and transvacuolar, that differ in their tension and response to both signaling molecules and reagents that perturb the cytoskeleton. It is further demonstrated that the tension of the transvacuolar strands can be significantly decreased by the addition of either linoleic acid, 1,2 dioctanoylsn-glycerol, or 1,3 dioctanoylglycerol. These decreases in tension could also be induced by lowering the cytoplasmic pH. In contrast, addition of Ca 2+, Mg 2+, or the ionophore A23187 to the cells caused a considerable increase in the tension of the transvacuolar strands. The data provides evidence that: (a) linoleic acid may be a signaling molecule in plant cells; (b) diacylglycerol functions as a signaling molecule through a protein kinase C-independent pathway mediated by PLA2; and (c) Ca 2+ and pH have regulatory roles for controlling cytoskeleton tension and organization.

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“…PA has also been shown to stimulate mammalian Nox activity in a cell-free system through direct interaction with gp91 phox , and in plant cells, by direct interaction with the Nox (Palicz et al, 2001;Qualliotine-Mann et al, 1993;Taylor et al, 2004Taylor et al, , 2012Zhang et al, 2009). While the E. festucae ΔpldB strains showed reduced NBT tip staining, indicative of reduced production of superoxide, this synthesis was confined to the mitochondria.…”

Section: The Genome Of E Festucae Encodes Two Putative Pc-hydrolysingmentioning

confidence: 99%

“…In animal cells PA has been found to interact with multiple components of the Nox complex, including the catalytic subunit gp91 phox (or Nox2) (Ago et al, 2003;Kanai et al, 2001;Karathanassis et al, 2002;Qualliotine-Mann, Agwu, Ellenburg, McCall, & McPhail, 1993;Taylor et al, 2004;Taylor, Riesselman, Lord, Gripentrog, & Jesaitis, 2012). In a cell-free system, PA was shown to be crucial for the activation of the mammalian Nox (Palicz, Foubert, Jesaitis, Marodi, & McPhail, 2001;Qualliotine-Mann et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Phosphatidic acid produced by phospholipase D is required for hyphal cell‐cell fusion and fungal‐plant symbiosis

Hassing

Eaton

Winter

et al. 2020

Molecular Microbiology

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Although lipid signaling has been shown to serve crucial roles in mammals and plants, little is known about this process in filamentous fungi. Here we analyze the contribution of phospholipase D (PLD) and its product phosphatidic acid (PA) in hyphal morphogenesis and growth of Epichloë festucae and Neurospora crassa, and in the establishment of a symbiotic interaction between E. festucae and Lolium perenne. Growth of E. festucae and N. crassa PLD deletion strains in axenic culture, and for E. festucae in association with L. perenne, were analyzed by light‐, confocal‐ and electron microscopy. Changes in PA distribution were analyzed in E. festucae using a PA biosensor and the impact of these changes on the endocytic recycling and superoxide production investigated. We found that E. festucae PldB, and the N. crassa ortholog, PLA‐7, are required for polarized growth and cell fusion and contribute to ascospore development, whereas PldA/PLA‐8 are dispensable for these functions. Exogenous addition of PA rescues the cell‐fusion phenotype in E. festucae. PldB is also crucial for E. festucae to establish a symbiotic association with L. perenne. This study identifies a new component of the cell‐cell communication and cell fusion signaling network for hyphal morphogenesis and growth of filamentous fungi.

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Phosphatidic acid and diacylglycerol synergize in a cell-free system for activation of NADPH oxidase from human neutrophils.

Cited by 89 publications

References 64 publications

Lipid Regulation of CTP:Phosphocholine Cytidylyltransferase: Electrostatic, Hydrophobic, and Synergistic Interactions of Anionic Phospholipids and Diacylglycerol

Lipid Regulation of CTP:Phosphocholine Cytidylyltransferase: Electrostatic, Hydrophobic, and Synergistic Interactions of Anionic Phospholipids and Diacylglycerol

Lipids trigger changes in the elasticity of the cytoskeleton in plant cells: a cell optical displacement assay for live cell measurements.

Phosphatidic acid produced by phospholipase D is required for hyphal cell‐cell fusion and fungal‐plant symbiosis

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