Phosphate uptake kinetics byAcinetobacter isolates

Pauli, Anneli; Kaitala, Seppo

doi:10.1002/(sici)1097-0290(19970205)53:3<304::aid-bit9>3.0.co;2-m

Cited by 12 publications

(5 citation statements)

References 25 publications

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“…The optimal cultivation time for P removal by M. circinelloides was 5 days. Organisms typically remove P through three patterns: by the overplus phenomenon after P limitation, in the declining phases of growth, or by luxury phosphate uptake in the growth phase without P starvation [15]. The growth curve showed that M. circinelloides accumulated polyphosphate during growth without P starvation, which implied that M. circinelloides accumulated P by luxury phosphate uptake.…”

Section: Neisser Stain To Confirm Phosphate Accumulation Of M Circinmentioning

confidence: 99%

“…The growth curve showed that M. circinelloides accumulated polyphosphate during growth without P starvation, which implied that M. circinelloides accumulated P by luxury phosphate uptake. Organisms with luxury phosphate uptake capability have gained great interest owning to their high P removal efficiencies [15]. Furthermore, this fungal strain can continuously uptake phosphate from media during its growth without the need for a complicated aeration operation (shaking at 150 rpm in this study).…”

Section: Neisser Stain To Confirm Phosphate Accumulation Of M Circinmentioning

confidence: 99%

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Screening of Phosphorus-Accumulating Fungi and Their Potential for Phosphorus Removal from Waste Streams

Gan

2015

Appl Biochem Biotechnol

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While bacteria have been primarily studied for phosphorus (P) removal in wastewater treatment, fungi and their ability to accumulate intracellular polyphosphate are less investigated. P-accumulating fungal strains were screened from soybean plants and surrounding soil by flask cultivation with potato dextrose broth and KH2PO4 in this study. Mucor circinelloides was selected for its high efficiency in P removal efficiency and high cellular P content. Neisser staining and growth-curve analysis confirmed that M. circinelloides stored polyphosphate intracellularly by luxury phosphate uptake. The effect of culture medium compositions on P removal efficiency and cellular P content was also investigated. Monosaccharides (such as glucose and fructose) and organic nitrogen (N, such as urea, and peptone) promoted fungi growth and P accumulation. M. circinelloides also preferred organic phosphates. When glucose, urea, and phytic acid sodium salt were used as the carbon, N, and P source, respectively, the maximum utilization efficiency was 40.1% for P and 7.08% for cellular P content. In addition, the potential of M. circinelloides for P removal from waste streams was investigated. Compared with the non-inoculated control culture, inoculation with M. circinelloides improved the soluble P removal in treating wastewater centrate, screened manure, and digested manure.

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Section: Neisser Stain To Confirm Phosphate Accumulation Of M Circinmentioning

confidence: 99%

Section: Neisser Stain To Confirm Phosphate Accumulation Of M Circinmentioning

confidence: 99%

Screening of Phosphorus-Accumulating Fungi and Their Potential for Phosphorus Removal from Waste Streams

Gan

2015

Appl Biochem Biotechnol

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“…Phosphate uptake kinetics by Acinetobacter isolates were studied by Pauli and Kaitala (1997). Ha]f-saturation constants were determined to be ] 7 and 0.4-6.0 mg/L as phosphorus for phosphate uptake and cell growth, respectively.…”

Section: Ks Coefficientsmentioning

confidence: 99%

“…In a pure culture study using A. twoffii and A. calcoaceticus, the phosphorus release under anaerobic conditions was not always related to the amount of VFA or phosphorus consumed, and phosphorus uptake under aerobic conditions was independent of phosphorus-release rates (Rustrian et at., 1997). Pauli and Kaitala (1997) studied phosphate uptake in Acinetobacter, and phosphate uptake followed Michaelis-Menten kinetics with a maximum uptake rate of 29 mg phosphorus/g SS' hand halfsaturation constant of ]7 mglL as phosphorus.…”

Section: Literature Review 1998mentioning

confidence: 99%

Activated sludge and other suspended culture processes

Ellis¹,

Higgins²,

Kaiser³

et al. 1998

Water Environment Research

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“…Typically, a simple unstructured kinetic model is used to describe the interaction between the substrate and the enzyme or microbe. The Michaelis-Menten equation has been widely used to describe substrate depletion by enzymes and nongrowing bacterial suspensions (Betlach and Tiedje, 1981;Pauli and Kaitala, 1997;and Suflita et al, 1983) while the Monod equation has been widely used to describe growth associated substrate consumption (Corman and Pave, 1983;Naziruddin et al, 1995;and Smith et al, 1997). Quantification of the microbe-or enzyme-substrate interaction involves estimating several parameters in the kinetic models using experimental data.…”

Section: Introductionmentioning

confidence: 99%

Nonlinear Estimation of Microbial and Enzyme Kinetic Parameters from Progress Curve Data

Goudar

Devlin²

2001

Water Environment Research

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A computer program (BIOKINFIT) was developed to estimate microbial and enzyme kinetic parameters from progress curve data by nonlinear regression. BIOKINFIT overcomes the limitations associated with parameter estimation in nonlinear equations that are implicit in the dependent variable by numerically approximating the substrate concentration in the integrated kinetic expressions. The simplex method was used to minimize the error between experimentally observed and theoretically predicted substrate depletion data. The robustness of this approach was initially verified using synthetic substrate depletion data that were characterized by either simple or relative errors of known magnitude. Subsequently, previously published data from three different experiments including the pyruvate kinase reaction, 2-chlorophenol biodegradation, and glucose uptake by Penicillium chrysogenum were used to verify the utility of the present approach for kinetic parameter estimation. In all cases, experimental substrate depletion data were accurately described by the theoretical curves and kinetic parameter estimates obtained in this study using the simplex method were in close agreement with those reported previously. BIOKINFIT, available free of charge on request, offers a convenient and robust method of analyzing progress curve data in implicit kinetic expressions. Water Environ. Res., 73, 260 (2001).Nomenclature K c ϭ Contois saturation constant K m ϭ Michaelis constant, M K s ϭ Monod half saturation coefficient, mg/L K ic ϭ competitive inhibition constant, mg/L K is ϭ substrate inhibition constant, mg/L K iu ϭ uncompetitive inhibition constant, mg/L S ϭ substrate concentration, mg/L S 0 ϭ initial substrate concentration, mg/L t ϭ time, hours v m ϭ maximum velocity, mg/L⅐h X ϭ biomass concentration, mg/L X 0 ϭ initial biomass concentration, mg/L Y ϭ yield coefficient, mg biomass/mg substrate m ϭ maximum specific growth rate, h Ϫ1

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Phosphate uptake kinetics byAcinetobacter isolates

Cited by 12 publications

References 25 publications

Screening of Phosphorus-Accumulating Fungi and Their Potential for Phosphorus Removal from Waste Streams

Screening of Phosphorus-Accumulating Fungi and Their Potential for Phosphorus Removal from Waste Streams

Activated sludge and other suspended culture processes

Nonlinear Estimation of Microbial and Enzyme Kinetic Parameters from Progress Curve Data

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