Phosphate Transport and Homeostasis in Arabidopsis

Poirier, Yves; Bucher, Marcel

doi:10.1199/tab.0024

Cited by 254 publications

(268 citation statements)

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“…Phosphorus (P) is an essential macronutrient for all living organisms (Poirier and Bucher 2002). In plants, it is present both as a constituent of such compounds as nucleic acids, phospholipids and ATP.…”

Section: Introductionmentioning

confidence: 99%

Evaluation and QTL mapping of phosphorus concentration in soybean seed

et al. 2012

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Phosphorus (P) is an essential macronutrient required for many biological and metabolic plant functions. Phosphate is the form of P used by plants.Reducing the amount of P in the seed can further aide breeding efforts to reduce the amount of P released into the environment due to the indigestibility of the complexes formed with metal ions. Analysis of the variation of phosphorus concentration in soybean seed under non-stressed conditions revealed that phosphorus ranged from 3,948.1 to 5,695.8 lg/g total phosphorous (TP) in combined years. The averages for independent years were significantly different from one another. Quantitative trait loci (QTL) analysis of TP was performed to identify candidate gene(s) that is (are) involved in P accumulation in soybean seed. One putative QTL region was identified on chromosome 12 in the combined data that contained a phosphate transporter gene. Two additional suggestive QTL were identified on chromosomes 7 and 17 with chromosome 7 having both a phosphate transport gene and a ZIP transporter gene in the region of the QTL. There were additional genes in these regions that are involved in phosphate metabolism and transport.

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Section: Introductionmentioning

confidence: 99%

Evaluation and QTL mapping of phosphorus concentration in soybean seed

et al. 2012

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“…Specific transport systems are essential for the uptake of Pi and for its partitioning within plants (Poirier and Bucher, 2002). Kinetic studies have shown the coexistence of two transport systems with low or high affinity for Pi (Dunlop et al, 1997;Misson et al, 2004;Miller et al, 2009).…”

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confidence: 99%

“…The primary source of P for plants is inorganic phosphate (Pi). Since Pi is often present in limited amounts in soils, it represents the second most frequently limiting factor for plant growth in a variety of natural ecosystems (Marschner, 1995;Poirier and Bucher, 2002;Misson et al, 2004). Pi concentration in the soil solution hardly reaches 1 mM in forest soils and may drop to micromolar or even submicromolar levels at the root-soil interface, where a depletion zone is generated by rapid Pi uptake of plants and rhizospheric microorganisms (Poirier and Bucher, 2002;Plassard and Dell, 2010).…”

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Structure and Expression Profile of the Phosphate Pht1 Transporter Gene Family in Mycorrhizal Populus trichocarpa

et al. 2011

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Gene networks involved in inorganic phosphate (Pi) acquisition and homeostasis in woody perennial species able to form mycorrhizal symbioses are poorly known. Here, we describe the features of the 12 genes coding for Pi transporters of the Pht1 family in poplar (Populus trichocarpa). Individual Pht1 transporters play distinct roles in acquiring and translocating Pi in different tissues of mycorrhizal and nonmycorrhizal poplar during different growth conditions and developmental stages. Pi starvation triggered the up-regulation of most members of the Pht1 family, especially PtPT9 and PtPT11. PtPT9 and PtPT12 showed a striking up-regulation in ectomycorrhizas and endomycorrhizas, whereas PtPT1 and PtPT11 were strongly down-regulated. PtPT10 transcripts were highly abundant in arbuscular mycorrhiza (AM) roots only. PtPT8 and PtPT10 are phylogenetically associated to the AM-inducible Pht1 subfamily I. The analysis of promoter sequences revealed conserved motifs similar to other AM-inducible orthologs in PtPT10 only. To gain more insight into gene regulatory mechanisms governing the AM symbiosis in woody plant species, the activation of the poplar PtPT10 promoter was investigated and detected in AM of potato (Solanum tuberosum) roots. These results indicated that the regulation of AM-inducible Pi transporter genes is conserved between perennial woody and herbaceous plant species. Moreover, poplar has developed an alternative Pi uptake pathway distinct from AM plants, allowing ectomycorrhizal poplar to recruit PtPT9 and PtPT12 to cope with limiting Pi concentrations in forest soils.

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“…Vacuoles are the major storage compartment for Pi in the cell (storing about 80% of the total cellular Pi) in nonlimiting conditions (Bieleski, 1983;Poirier and Bucher, 2002). Pi incorporated in lipid membranes also represents an abundant and readily available source.…”

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The Phosphate Fast-Responsive Genes PECP1 and PPsPase1 Affect Phosphocholine and Phosphoethanolamine Content

et al. 2018

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Phosphate starvation-mediated induction of the HAD-type phosphatases PPsPase1 (AT1G73010) and PECP1 (AT1G17710) has been reported in Arabidopsis (Arabidopsis thaliana). However, little is known about their in vivo function or impact on plant responses to nutrient deficiency. The preferences of PPsPase1 and PECP1 for different substrates have been studied in vitro but require confirmation in planta. Here, we examined the in vivo function of both enzymes using a reverse genetics approach. We demonstrated that PPsPase1 and PECP1 affect plant phosphocholine and phosphoethanolamine content, but not the pyrophosphate-related phenotypes. These observations suggest that the enzymes play a similar role in planta related to the recycling of polar heads from membrane lipids that is triggered during phosphate starvation. Altering the expression of the genes encoding these enzymes had no effect on lipid composition, possibly due to compensation by other lipid recycling pathways triggered during phosphate starvation. Furthermore, our results indicated that PPsPase1 and PECP1 do not influence phosphate homeostasis, since the inactivation of these genes had no effect on phosphate content or on the induction of molecular markers related to phosphate starvation. A combination of transcriptomics and imaging analyses revealed that PPsPase1 and PECP1 display a highly dynamic expression pattern that closely mirrors the phosphate status. This temporal dynamism, combined with the wide range of induction levels, broad expression, and lack of a direct effect on Pi content and regulation, makes PPsPase1 and PECP1 useful molecular markers of the phosphate starvation response.Plant growth is highly sensitive to a lack of phosphate (Pi); hence, the application of Pi fertilizers has become standard practice for high-throughput crop production (Cordell et al., 2009). Most phosphorus in the soil is not available to plants, as it is combined with other minerals or parts of organic compounds (Bieleski, 1973;Raghothama and Karthikeyan, 2005). Only a small fraction of soluble Pi (usually present at a concentration of ,10 mM in the soil) can be taken up by plants.Although growth is not optimal under limiting conditions, plants can withstand changing Pi concentrations within heterogeneous soils or in the external nutrient supply that can lead to reprogramming of their metabolism and architecture (Hammond et al., 2003;Péret et al., 2011;Plaxton and Tran, 2011). Root architecture can be modified to facilitate Pi uptake by favoring the development of lateral roots (at the expense of primary root elongation in many plants including Arabidopsis), increasing the density and length of root hairs, and limiting the development of aerial parts (López-Bucio et al., 2002;Svistoonoff et al., 2007;Gruber et al., 2013). Pi uptake mechanisms are enhanced at the root/soil interface, particularly through the stimulation of Pi transport activity (Mudge et al., 2002;Shin et al., 2004;Nussaume et al., 2011; Ayadi et al., 2015). In parallel, mobilization of Pi sources fr...

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Phosphate Transport and Homeostasis in Arabidopsis

Cited by 254 publications

References 150 publications

Evaluation and QTL mapping of phosphorus concentration in soybean seed

Evaluation and QTL mapping of phosphorus concentration in soybean seed

Structure and Expression Profile of the Phosphate Pht1 Transporter Gene Family in Mycorrhizal Populus trichocarpa

The Phosphate Fast-Responsive Genes PECP1 and PPsPase1 Affect Phosphocholine and Phosphoethanolamine Content

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