Phosphate Starvation Triggers Transcriptional Changes in the Biosynthesis and Signaling Pathways of Phytohormones in Marchantia polymorpha

Rico-Reséndiz, Félix; Diaz-Santana, Zazil Ha Uc; Dipp-Álvarez, Melissa; Cruz-Hernández, Andrés; Bowman, John L.; Herrera-Estrella, Luis; Ishizaki, Kimitsune; Arteaga-Vázquez, Mario; Cruz‐Ramírez, Alfredo

doi:10.3390/iecps2020-08729

Cited by 3 publications

(2 citation statements)

References 32 publications

(59 reference statements)

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“…Whether these stress responses are activated in M. polymorpha in an ethylene-dependent manner remains to be studied, but initial genomic and transcriptomic analyses of salt- and heat-stressed M. polymorpha plants revealed that genes involved in oxidative stress (ROS metabolism) were conserved and enriched ( Tanaka et al., 2018 ; Marchetti et al., 2021 ). Additionally, transcriptional changes in ethylene signaling genes have been observed under conditions of phosphate starvation in M. polymorpha ( Rico-Resendiz et al., 2021 ), although the phenotypic consequences remain unknown.…”

Section: Discussionmentioning

confidence: 99%

The plant hormone ethylene promotes abiotic stress tolerance in the liverwort Marchantia polymorpha

Bharadwaj

Sanchez²,

Li³

et al. 2022

Front. Plant Sci.

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Plants are often faced with an array of adverse environmental conditions and must respond appropriately to grow and develop. In angiosperms, the plant hormone ethylene is known to play a protective role in responses to abiotic stress. Here we investigated whether ethylene mediates resistance to abiotic stress in the liverwort Marchantia polymorpha, one of the most distant land plant relatives of angiosperms. Using existing M. polymorpha knockout mutants of Mpein3, and Mpctr1, two genes in the ethylene signaling pathway, we examined responses to heat, salinity, nutrient deficiency, and continuous far-red light. The Mpein3 and Mpctr1 mutants were previously shown to confer ethylene insensitivity and constitutive ethylene responses, respectively. Using mild or sub-lethal doses of each stress treatment, we found that Mpctr1 mutants displayed stress resilience similar to or greater than the wild type. In contrast, Mpein3 mutants showed less resilience than the wild type. Consistent with ethylene being a stress hormone, we demonstrated that ethylene production is enhanced by each stress treatment. These results suggest that ethylene plays a role in protecting against abiotic stress in M. polymorpha, and that ethylene has likely been conserved as a stress hormone since before the evolutionary divergence of bryophytes from the land plant lineage approximately 450 Ma.

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Section: Discussionmentioning

confidence: 99%

The plant hormone ethylene promotes abiotic stress tolerance in the liverwort Marchantia polymorpha

Bharadwaj

Sanchez²,

Li³

et al. 2022

Front. Plant Sci.

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show abstract

“…Recently, it was shown that ethylene signaling and biosynthesis is key to tolerate heat, salt, nutrient and light stress in the liverwort Marchantia polymorpha ( Bharadwaj et al, 2022 ). Phosphate starvation also induced transcriptional changes of ethylene-related genes in Marchantia ( Rico-resendiz et al, 2020 ). Submergence tolerance was observed to be mediated by ethylene signaling in the moss Physcomitrium patens ( Yasumura et al, 2012 ).…”

Section: The Evolutionary Role Of Ethylene In Abiotic Stress Responsesmentioning

confidence: 99%

Evolution of ethylene as an abiotic stress hormone in streptophytes

Van de Poel,

de Vries

2023

Environmental and Experimental Botany

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Systemic response to nitrogen starvation in the cyanobacteria hosting hornwort and liverwort provides little evidence for extensive priming to the cyanobiont

Yue,

Sablok,

Neubauer

et al. 2024

Preprint

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Mutualistic plant-microbe symbiotic interactions are thought to have evolved from a loose association often observed between host plants and microbes when nutrients are limited and caused by chemical attractants released by the host plant. Therefore, the molecular network enabling intimate mutualistic plant-microbe symbioses may have evolved from a general nutrient starvation response shared by all land plants (embryophytes). Nevertheless, this hypothesis remains to be tested because information on the consequences of nutrient starvation is mainly available for vascular plants but missing for most non-vascular plant lineages. While well-studied in vascular plants, particularly in arbuscular mycorrhizal fungi- and nodule-forming bacteria-plant symbioses, the molecular link between nutrient status and symbiotic interaction remains poorly understood in other systems. This is especially true to the symbiotic associations between plants and cyanobacteria, in which host plants release so-called hormogonia inducing factors (HIF) only under nutrient limited conditions to mobilize the cyanobiont and initiate the interaction. Nevertheless, in contrast to the well-investigated mycorrhiza- and nodule-forming bacteria-plant associations, plant-cyanobacteria interactions are extracellular and morphological structures hosting the cyanobionts are formed even in the absence of the microbial partner. Therefore, nutrient starvation may induce a less complex genetic network in plant-cyanobacteria systems in contrast to intracellular symbioses (plant-AMF/nodule-forming bacteria). To test the hypothesis of a conserved starvation network across land plants and to investigate the link between nutrient starvation and symbiosis initiation in the plant-cyanobacteria symbiosis, we explore the transcriptional responses to nutrient starvation in two non-vascular plant species, a hornwort Anthoceros agrestis and a liverwort Blasia pusilla, serving as ideal model systems for plant-cyanobacteria endophytic symbioses. By analyzing time-series RNA-seq data, we investigate gene expression changes associated with nutrient starvation in these bryophyte species and compare them with data available for vascular plants. Our study aims to identify convergent and divergent transcriptomic responses and to uncover transcriptomic signatures specific to cyanobacteria symbiosis-capable organisms. Our results reveal that A. agrestis and B. pusilla exhibit similar transcriptional responses to the nitrogen starvation, characterized by the upregulation of genes involved in nitrogen uptake and assimilation, hormonal regulation, stress response, and signal transduction pathways. Moreover, our findings suggest that bioactive molecules, particularly flavonoids produced under nutrient starvation, may play a role in initiating the plant-mycorrhiza and nodule-forming bacteria symbioses, particularly serving as HIF in plant-cyanobacteria symbioses.

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Phosphate Starvation Triggers Transcriptional Changes in the Biosynthesis and Signaling Pathways of Phytohormones in Marchantia polymorpha

Cited by 3 publications

References 32 publications

The plant hormone ethylene promotes abiotic stress tolerance in the liverwort Marchantia polymorpha

The plant hormone ethylene promotes abiotic stress tolerance in the liverwort Marchantia polymorpha

Evolution of ethylene as an abiotic stress hormone in streptophytes

Systemic response to nitrogen starvation in the cyanobacteria hosting hornwort and liverwort provides little evidence for extensive priming to the cyanobiont

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