Phosphate starvation is accompanied by disturbance of intracellular cysteine homeostasis in Escherichia coli

Смирнова, Г. В.; Tyulenev, Aleksey V.; Bezmaternykh, K. V.; Muzyka, Nadezda G.; Ushakov, Vadim Y.; Oktyabrsky, Oleg N.

doi:10.1016/j.resmic.2023.104108

Cited by 2 publications

(8 citation statements)

References 52 publications

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“…The absence of one of these mechanisms of cysteine homeostasis in mutants can be compensated to some extent by other mechanisms when E. coli grows in minimal sulfate medium. However, glutathione deficiency in the gshA mutant led to an increase in the level of intracellular cysteine under stress, stimulated longer-term sulfide production and significantly increased the sensitivity of this strain to H2O2 [7,[18][19][20]. Protective role of the cysteinecystine shuttle system, induced in E. coli under peroxide stress, was also previously shown [21].…”

Section: Introductionmentioning

confidence: 54%

“…Incorporation into glutathione is one of the main mechanisms for the removal of excess cysteine during stress-induced disturbances of its homeostasis in E. coli growing in minimal media [7,20]. In this work, we studied the role of glutathione in maintaining cysteine homeostasis during normal growth and under the influence of antibiotics in LB medium.…”

Section: Effect Of Mutations In Cysteine Homeostasis On Changes In In...mentioning

confidence: 99%

“…The appearance of excess cysteine in the cytoplasm can occur when cystine or cysteine is added to cells growing in minimal sulfate medium [3,8], or under various starvation stresses and treatment of E. coli with antibiotics (chloramphenicol, tetracycline, ciprofloxacin), causing a sharp inhibition of growth and protein synthesis [7,[18][19][20]. Under stress conditions, disruption of cysteine homeostasis is accompanied by a rapid increase in intracellular reduced glutathione (GSH), release of cysteine into the medium, and transient H2S production.…”

Section: Introductionmentioning

confidence: 99%

“…This may be a consequence of differences in the maintenance of cysteine homeostasis under conditions where the main source of cysteine is either its synthesis using sulfate or the import of cystine from the environment. Previously, we studied in detail changes in the levels of cysteine, glutathione and H2S under stresses, including the action of antibiotics, in E. coli growing in minimal M9 medium [7,9,20]. The purpose of this work was to study the features of cysteine homeostasis in E. coli growing in the widely used rich Luria-Bertani (LB) medium under normal conditions and under antibiotic-induced stress.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Regulation of cysteine homeostasis and its effect on Escherichia coli sensitivity to ciprofloxacin in LB medium

Smirnova,

Tyulenev,

Sutormina

et al. 2024

Preprint

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Cysteine and its derivatives, including H2S, can influence bacterial virulence and sensitivity to antibiotics. In minimal sulfate media, H2S is generated under stress to prevent excess cysteine and, together with incorporation into glutathione and export into the medium, is a mechanism of cysteine homeostasis. Here, we studied the features of cysteine homeostasis in LB medium, where the main source of sulfur is cystine, whose import can create excess cysteine inside cells. We used mutants in the mechanisms of cysteine homeostasis and a set of microbiological and biochemical methods, including real-time monitoring of sulfide and oxygen, determination of cysteine and glutathione, and expression of the Fur, OxyR, and SOS regulons genes. During normal growth, the parental strain generated H2S when switching respiration to another substrate. The mutations affected the onset time, intensity and duration of H2S production, cysteine and glutathione levels, bacterial growth and respiration rates, and induction of defense systems. Exposure to chloramphenicol and high doses of ciprofloxacin increased cysteine content and GSH synthesis. A high inverse relationship between log CFU/ml and bacterial growth rate before ciprofloxacin addition was revealed. The study points to the important role of maintaining cysteine homeostasis during normal growth and antibiotic exposure in LB medium.

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Section: Introductionmentioning

confidence: 54%

Section: Effect Of Mutations In Cysteine Homeostasis On Changes In In...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Regulation of cysteine homeostasis and its effect on Escherichia coli sensitivity to ciprofloxacin in LB medium

Smirnova,

Tyulenev,

Sutormina

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…The appearance of excess cysteine in the cytoplasm can occur when cystine or cysteine is added to cells growing in minimal sulfate medium [3,8], or under various starvation stresses and the treatment of E. coli with antibiotics (chloramphenicol, tetracycline, ciprofloxacin), causing a sharp inhibition of growth and protein synthesis [7,[18][19][20]. Under stress conditions, the disruption of cysteine homeostasis is accompanied by a rapid increase in intracellular reduced glutathione, the release of cysteine into the medium, and transient H 2 S production.…”

Section: Introductionmentioning

confidence: 99%

Regulation of Cysteine Homeostasis and Its Effect on Escherichia coli Sensitivity to Ciprofloxacin in LB Medium

Smirnova,

Tyulenev,

Sutormina

et al. 2024

IJMS

Self Cite

View full text Add to dashboard Cite

Cysteine and its derivatives, including H2S, can influence bacterial virulence and sensitivity to antibiotics. In minimal sulfate media, H2S is generated under stress to prevent excess cysteine and, together with incorporation into glutathione and export into the medium, is a mechanism of cysteine homeostasis. Here, we studied the features of cysteine homeostasis in LB medium, where the main source of sulfur is cystine, whose import can create excess cysteine inside cells. We used mutants in the mechanisms of cysteine homeostasis and a set of microbiological and biochemical methods, including the real-time monitoring of sulfide and oxygen, the determination of cysteine and glutathione (GSH), and the expression of the Fur, OxyR, and SOS regulons genes. During normal growth, the parental strain generated H2S when switching respiration to another substrate. The mutations affected the onset time, the intensity and duration of H2S production, cysteine and glutathione levels, bacterial growth and respiration rates, and the induction of defense systems. Exposure to chloramphenicol and high doses of ciprofloxacin increased cysteine content and GSH synthesis. A high inverse relationship between log CFU/mL and bacterial growth rate before ciprofloxacin addition was revealed. The study points to the important role of maintaining cysteine homeostasis during normal growth and antibiotic exposure in LB medium.

show abstract

Phosphate starvation is accompanied by disturbance of intracellular cysteine homeostasis in Escherichia coli

Cited by 2 publications

References 52 publications

Regulation of cysteine homeostasis and its effect on Escherichia coli sensitivity to ciprofloxacin in LB medium

Regulation of cysteine homeostasis and its effect on Escherichia coli sensitivity to ciprofloxacin in LB medium

Regulation of Cysteine Homeostasis and Its Effect on Escherichia coli Sensitivity to Ciprofloxacin in LB Medium

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