Phosphate-specific transport system of Escherichia coli: nucleotide sequence and gene-polypeptide relationships

Abstract

The DNA nucleotide sequence of four genes for the phosphate-specific transport system of Escherichia coli is reported. Along with the DNA sequence for the phoS gene reported previously (Surin et al., J. Bacteriol. 157:772-778, 1984; Magota et al., J. Bacteriol. 157:909-917, 1984), this study completes the nucleotide sequence of the phosphate-specific transport region. The complete sequence (including phoS) contains five open reading frames oriented in the same direction, each preceded by a putative ribosome-binding site near the presumed translation initiation codon ATG. The complete sequence is transcribed counterclockwise, in the order phoS psiC pstA pstB phoU. Genetic complementation shows that of the four open reading frames in the new sequence, three correspond to known mutant alleles; the fourth, which was designated psiC, has not been described before and could not be related to any known mutant allele. We have confirmed that pstA was allelic to phoT32. The pstC, pstB, and phoU gene products were identified as peripheral membrane proteins. The pstA gene product appears to be an integral membrane protein. 189 on July 6, 2020 by guest

The distribution of positively charged residues in bacterial inner membrane proteins correlates with the trans-membrane topology

“…The topology is indicated by showing the number of positive and negative residues in each connecting loop in its proper cytosolic or periplasmic location, starting from the N-terminus. Loops in square brackets are not included in the amino acid statistics since they are longer than 65 residues Michel et al (1985) Michel et al (1986) (+1/-1) (+0/-4)-C (+2/-4) Michel et al (1986) (+ 1/-2) (+0/-2)-C N-(+ 1/-3) Dunn et al (1981) Drews (1985) Drews (1985) Froshauer and Beckwith (1984) [+ 18/-21] Buchel et al (1980) (+0/-0) Wolfe et al (1983) [ +25/-301-C 3022 Gay and Walker (1981) Gay and Walker (1981) Grundstrom and Jaurin (1982) Surin et al (1985) 3023 peak hydrophobicity distribution for the inner membrane proteins (corresponding to connecting loops and spanning segments) stands out clearly. Due to the poor separation between the two peaks, however, segments with a peak hydrophobicity value in the range 0.8-1.4 cannot be unambiguously assigned to any of the two groups.…”

A mycoplasma high-affinity transport system and the in vitro invasiveness of mouse sarcoma cells.

“…No significant sequence similarities of p37 and p69 with other known proteins were found. However, extensive similarity of p29 with the bacterial proteins hisP of Salmonella typhimurium (Higgins et al, 1982), malK of E. coli (Gilson et al, 1982), oppD of S. typhimurium (Higgins et al, 1985) and the more recently published pstB of E. coli (Surin et al, 1985) was found (Figure 3). These fairly hydrophilic peripheral membrane proteins are part of the periplasmic bindingprotein-dependent multicomponent transport systems for histidine, arginine, ornithine and lysine (his), for maltose and maltodextrins (mal), for phosphate (pst), and for oligopeptides (opp) of Gram-negative bacteria (reviewed in Ames, 1986).…”

An ABC transporter in the mitochondrial inner membrane is required for normal growth of yeast.

“…. The sequences were from the following sources: Ste6p (Kuchler et al, 1989;McGrath and Varshavsky, 1989), Mdrl (Chen et al, 1986), HlyB (Felmlee et al, 1985), HisP (Higgins et al, 1982), MalK (Gilson et al, 1982), PstB (Surin et al, 1985), OppF and OppD (Hiles et al, 1987). Conserved amino acids are printed in bold.…”