Phosphate Solubilizing Bacteria Adaptive to Vinasse

Muzakhar, Kahar; Sutoyo, Sutoyo; Saragih, Ahmad Bukhari

doi:10.5614/j.math.fund.sci.2015.47.2.8

Cited by 8 publications

(4 citation statements)

References 6 publications

(13 reference statements)

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“…This enzyme has been widely used in several industrial applications as a catalyst (Habelt & Pittner, 1983;Bokkenheuser et al, 1987;Caldini et al, 1994;Wang et al, 2005;Birgisson et al, 2007). The application of enzymes in the bioconversion process is the veracious strategy because it does not harm either have no negative impact to the environment and further its stability of the biodiversity in ecosystem function will always be maintained (Prakasham et al, 2007;Muzakhar et al, 2015;Muzakhar et al, 2017). However, to produce enzyme still requires a quite costly process including the materials either purification process.…”

Section: Introductionmentioning

confidence: 99%

Activity of an A-L-Rhamnosidase Produced by Aspergillus niger During Solid State Fermentation of Coffee Pulp Wastes

Muzakhar

Winarsa

2019

Jurnal Biodjati

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An α-L-Rhamnosidase released by Aspergillus niger during solid-state fermentation (SSF) using coffee pulp (CP) wastes media has been investigated. The activity of α-L-Rhamnosidase based on reducing sugar production against 2% CP alkali extract substrate in 50 mM acetate buffer pH 5. The maximum activity of α-L-Rham-nosidase was obtained in sixth-day SSF with reducing sugar pro-duction of 13 μg/mL. The enzyme is actively hydrolyzed 0.1% p-ni-trophenyl-α-L-rhamnopyranoside (PNP-Rha) to 95% from initial concentration. Purification using DEAE-Toyopearl 650M increased hydrolysis activity ten times against the substrate, reaching 134 μg/mL of reducing sugar. Optimum enzyme activity at pH 4.5 and 50°C, while stable at pH and temperature in a pH range of 3.5-7 and below 50°C.

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Section: Introductionmentioning

confidence: 99%

Activity of an A-L-Rhamnosidase Produced by Aspergillus niger During Solid State Fermentation of Coffee Pulp Wastes

Muzakhar

Winarsa

2019

Jurnal Biodjati

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“…Solid-state fermentation for cellulase production by Isolate VT11. Solid-state fermentation media made by 10 grams of coffee pulp with 20 mL of water content (1:2) (%w/v) [14]. The water content of coffee pulp is based on the previous result of coffee pulp water content calculation.…”

Section: Methodsmentioning

confidence: 99%

Preliminary Investigation of Cellulase Producer Candidate Isolate VT11 Using Coffee Pulp Waste Under Solid-State Fermentation

Sunarto

Azizah

Utarti

et al. 2021

KEM

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Isolate VT11 is a fungal cellulolytic isolated from vermicomposting oil palm empty fruit bunch (OPEFB). Isolate VT11 has a cellulolytic activity index of 1.0 on 1% CMC, but this isolate has never been used to produce cellulase from coffee pulp waste. The coffee pulp consists of organic components with the highest cellulose content (63%) so that it can be used as a substrate for cellulase production by fungal cellulolytic under solid-state fermentation (SSF). This study aims to know the potential of isolate VT11 as a candidate for cellulase producer. The potential of isolate VT11 as a candidate for cellulase producer was known by the cellulase activity on coffee pulp waste under solid-state fermentation. After that, cellulase was characterized by pH optimization and stabilization. Cellulase production was done by inoculating isolate VT11 in 10 g solid substrate of coffee pulp. The result shows that the isolate VT11 can potentially produce cellulase with the highest enzyme activity of 1.857 U/mL after 96 hours of incubation at 30 °C. Cellulase from isolate VT11 is optimal at pH 4.5 and stable at pH 5-6.5. Based on this result, it is suggested that the isolate VT11 can be used for cellulase production using coffee pulp waste as substrate agro-industrial residues. Further investigation such as species identification of isolate VT11, purification, and characterization of cellulase produced by isolate VT11 was needed.

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“…Cellulase has been produced from various microorganisms, especially fungi and bacteria [3,4]. The enzyme activity produced by fungi is higher than obtained by yeast and bacteria, so commercial enzyme production by fungi is preferred [5].…”

Section: Introductionmentioning

confidence: 99%

Cheap Cellulase Production by <i>Aspergillus</i> sp. VTM1 Through Solid State Fermentation of Coffee Pulp Waste

Rusdianti

Azizah

Utarti

et al. 2021

KEM

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Coffee pulp biomass waste can easily be found anywhere in Indonesia, considering it is the fourth world's largest coffee exporter. The utilization of coffee pulp is very limited and is categorized as a source of pollutants in water bodies and soils. In contrast, coffee pulp waste is very potential because 63% of the main compound is cellulose. Microbial utilization of this waste for enzyme production purposes, especially cellulase, is a breakthrough that may lead to reduce production costs. Initial investigations showed that Aspergillus sp. VTM1 through solid-state fermentation (SSF) could produce cellulases. Optimal cellulase could be produced if 10 g coffee pulp with 10% moisture is inoculated using 108 spores/mL of Aspergillus sp. VTM1 for 48 hours at 30 °C. Hydrolysis of 1% carboxymethyl cellulose (CMC) substrate in 50 mM acetate buffer pH 5 by this cellulase showed that the enzyme activity reached up to 1.18 U/mL. The optimum pH of the enzyme was 5 and stable at 3-3.5 and 4-7. The success of the first step of this investigation will be a cheap way of producing cellulases.

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Phosphate Solubilizing Bacteria Adaptive to Vinasse

Cited by 8 publications

References 6 publications

Activity of an A-L-Rhamnosidase Produced by Aspergillus niger During Solid State Fermentation of Coffee Pulp Wastes

Activity of an A-L-Rhamnosidase Produced by Aspergillus niger During Solid State Fermentation of Coffee Pulp Wastes

Preliminary Investigation of Cellulase Producer Candidate Isolate VT11 Using Coffee Pulp Waste Under Solid-State Fermentation

Cheap Cellulase Production by <i>Aspergillus</i> sp. VTM1 Through Solid State Fermentation of Coffee Pulp Waste

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