Phosphate Solubilization Activity of Rhizobial Strains Isolated From Root Nodule of Cluster Bean Plant Native to Indian Soils

Dhull, Subha; Gera, Rajesh; Sheoran, Hardeep Singh; Kakar, Ridham

doi:10.20546/ijcmas.2018.704.029

Cited by 6 publications

(4 citation statements)

References 18 publications

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“…Analysis of phosphate solubilization activity conducted by refers to the study of Pikovskaya [21]. After growing isolates in AG broth media for 3 days, 5 𝜇L of each isolate culture was drip on paper disc and it was placed on Pikovskaya's agar media for 24 -48 h [22]. Then, the clear zone that appears was observed and calculated to determine the phosphate solubilization index (PSI) using the equation below [23]:…”

Section: Phosphate Solubilization Activitymentioning

confidence: 99%

Bioprospecting of Rhizobia as Plant Growth Promoting Rhizobacteria Potential from Root Nodules of Groundnut (Arachis hypogaea L.)

Wulandari,

Baskoro,

Mahmuudah

et al. 2024

Trends Sci

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Rhizobia are bacteria that symbiosis with host plant, as shown in the root nodules formation, and provide nitrogen that can be absorbed by plants in greater quantities than rhizobacteria. Available Nitrogen, which absorbed by plants, is the essential requirement for plant growth because its role in increasing yield and quality, hence it is needed in greater quantities than other nutrients. The study aimed to determine the macroscopic and microscopic diversity of rhizobia isolates from the groundnut nodules and their potential as PGPRs, and to identify 16S rRNA isolates with the best potential as PGPRs molecularly. The methods used were isolation from root nodules, screening of PGPR potential, molecular identification based on the 16S rRNA gene, and phylogenetic analysis to determine their kinship. Based on the isolation results, 17 Gram-negative isolates were obtained white to pink or orange color on AG media with various colony characteristics in terms of shape, margin, elevation, and texture. KT 20, which was selected as rhizobia isolate with the best potential as PGPR, has ammonium concentration of 23.12 ppm, synthesizes IAA with a concentration of 10.36 ppm, and phosphates solubilization activity, although its ability to synthesize proteases is low. The results of molecular identification of 16S rRNA showed that KT 20 belongs to the Rhizobium genus with a similarity of 99.48 % and bootstrap value of 96 %. HIGHLIGHTS Isolate KT 20 (identified rhizobia) has excellent ability in fixing nitrogen, which plants needs the most and legumes requires in large number Beside the ability of Isolate KT 20 to fix nitrogen, it also able to synthesize IAA, solubilize phosphate, and synthesize protease The ability of isolate KT 20 as PGPR was equal or better than other rhizobacteria that has been analyzed in other in vitro studies GRAPHICAL ABSTRACT

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Section: Phosphate Solubilization Activitymentioning

confidence: 99%

Bioprospecting of Rhizobia as Plant Growth Promoting Rhizobacteria Potential from Root Nodules of Groundnut (Arachis hypogaea L.)

Wulandari,

Baskoro,

Mahmuudah

et al. 2024

Trends Sci

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“…There are microorganisms abundant in soil that can solubilize and mobilize P remaining in soil in unavailable form and thus plants get nutrition. Some P solubilizing Microorganisms are species of Agrobacterium, Aspergillus, Bacillus, Burkholderia, Enterobacter, Erwinia, Flavobacterium, Micrococcus, Pantoea, Pseudomonas, Rhizobium, Penicillium [109][110][111][112][113]. Among these different strains of Bacillus like B. circulans, B. megaterium, B. polymyxa, B. sircalmous and B. subtilis are important.…”

Section: Nutrient Assimilation and Biofortificationmentioning

confidence: 99%

Bioinoculants—Natural Biological Resources for Sustainable Plant Production

et al. 2021

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Agricultural sustainability is of foremost importance for maintaining high food production. Irresponsible resource use not only negatively affects agroecology, but also reduces the economic profitability of the production system. Among different resources, soil is one of the most vital resources of agriculture. Soil fertility is the key to achieve high crop productivity. Maintaining soil fertility and soil health requires conscious management effort to avoid excessive nutrient loss, sustain organic carbon content, and minimize soil contamination. Though the use of chemical fertilizers have successfully improved crop production, its integration with organic manures and other bioinoculants helps in improving nutrient use efficiency, improves soil health and to some extent ameliorates some of the constraints associated with excessive fertilizer application. In addition to nutrient supplementation, bioinoculants have other beneficial effects such as plant growth-promoting activity, nutrient mobilization and solubilization, soil decontamination and/or detoxification, etc. During the present time, high energy based chemical inputs also caused havoc to agriculture because of the ill effects of global warming and climate change. Under the consequences of climate change, the use of bioinputs may be considered as a suitable mitigation option. Bioinoculants, as a concept, is not something new to agricultural science, however; it is one of the areas where consistent innovations have been made. Understanding the role of bioinoculants, the scope of their use, and analysing their performance in various environments are key to the successful adaptation of this technology in agriculture.

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“…El potencial de solubilización de fosfato de cepas de rizobio se asocia con la producción de ácido 2-cetoglucónico y reducción del pH del medio (Hussain et al, 2018). La disminución del pH en la solubilización de fosfato por rizobios ha sido informada por varios autores, como Dhull et al (2018) quienes mencionaron el potencial de rizobios aislados de Cyamopsis tetragonoloba L. para solubilizar fósforo insoluble con eficiencias entre 36 a 79% y reducción del pH.…”

Section: Solubilización De Fosfatosunclassified

“…Asimismo, señalaron cepas de Rhizobium aisladas de Cicer arietinum (Joshi et al, 2016), de Cyamopsis tetragonoloba (Dhull et al, 2018), como productoras de sideróforos. Ullah et al (2017) mencionaron a Rhizobium leguminosarum y Mesorhizobium ciceri como resistentes a la sequía, además de ser productoras de sideróforos, AIA y ácidos orgánicos.…”

Section: Producción De Sideróforosunclassified

Mecanismos de inducción de rizobios para reducir el estrés por sequía en las leguminosas

Santillana

2021

Rev. investig. altoandin.

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Drought is one of the main limitations of agricultural productivity and food security, in Andean mountain. The use of atmospheric nitrogen-fixing rhizobia in symbiosis with legumes, and tolerant to a wide range of adverse conditions, such as drought, is a great potential in sustainable agriculture. The aim of this review is to compile studies about drought stress effect on the legume-rhizobia symbiosis and rhizobia mechanisms to induce drought tolerance in legumes. The search for information was conducted from August to December 2020, using key terms. The drought effect on the nodulation and atmospheric nitrogen fixation process is made known, as well as the rhizobia ability to synthesize exopolysaccharides, enzymes, phytohormones, siderophores, osmolytes and solubilize phosphates as induction mechanisms to mitigate drought stress in legumes. This review will serve to propose future research using rhizobia to mitigate the drought effect on the legumes cultivation in environments such as the Andean mountains.

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Phosphate Solubilization Activity of Rhizobial Strains Isolated From Root Nodule of Cluster Bean Plant Native to Indian Soils

Cited by 6 publications

References 18 publications

Bioprospecting of Rhizobia as Plant Growth Promoting Rhizobacteria Potential from Root Nodules of Groundnut (Arachis hypogaea L.)

Bioprospecting of Rhizobia as Plant Growth Promoting Rhizobacteria Potential from Root Nodules of Groundnut (Arachis hypogaea L.)

Bioinoculants—Natural Biological Resources for Sustainable Plant Production

Mecanismos de inducción de rizobios para reducir el estrés por sequía en las leguminosas

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