Phosphate-sensitive enzyme electrode: a potential sensor for environment control

D'Urso, Edith Marie; Coulet, Pierre R.

doi:10.1016/s0003-2670(00)83827-1

Cited by 55 publications

(11 citation statements)

References 11 publications

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“…Biosensors are expected to be simple, selective, economical, and ecologically sound [237]. Several biosensors have been developed on the basis of inhibition of alkaline phosphatase [238] or inhibition of a combination of nucleoside phosphatase and xanthine oxidase [239]. An FIA system based on the latter combination has also been developed using analyte recycling [240].…”

Section: Phosphate Ion Sensorsmentioning

confidence: 99%

Current research activity in biosensors

Nakamura

Karube

2003

Analytical and Bioanalytical Chemistry

259

129

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Biosensors consist of a molecular recognition element and a transducer. Since the first biosensor was developed by Updike and Hicks many biosensors and their associated techniques have been studied and developed. In this review current research activity on the fundamentals and applications of biosensors is summarized. In discussion of the former, molecular recognition elements, techniques and tools for biosensor construction, and basic biosensor devices are introduced. Coverage of the latter includes description of biosensors for environmental, food, and clinical fields. Chemical sensors developed in our laboratory for environmental monitoring are also described. This review mainly summarizes work performed by our group and by our colleagues, and refers to the main review articles summarizing each field of biosensor research in recent years.

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Section: Phosphate Ion Sensorsmentioning

confidence: 99%

Current research activity in biosensors

Nakamura

Karube

2003

Analytical and Bioanalytical Chemistry

259

129

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“…A variety of electrode substrates and analytical methods have been reported for the determination of phosphates as it is shown in Figure S1. Such approaches have utilised metal electrodes, ion selective membranes enzyme based electrodes, [25][26][27] gold and glassy carbon modified electrodes, 28 modified carbon paste electrodes, 27,29 cobalt wire ion selective electrodes, 30,31 lead ion selective electrodes, 32 and cobalt phthalocyanide modified screen-printed electrodes as amperometric sensors. 33,34 Consequently, in this work, the established EPA recommended colorimetric protocol by using molybdate ions as complexing agent is electrochemically adapted alleviating the need for the use of ascorbic acid and antimony potassium tartrate as reducing agents.…”

mentioning

confidence: 99%

Rapid and Portable Electrochemical Quantification of Phosphorus

2015

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Phosphorus is one of the key indicators of eutrophication levels in natural waters where it exists mainly as dissolved phosphorus. Various analytical protocols exist to provide an offsite analysis, and a point of site analysis is required. The current standard method recommended by the Environmental Protection Agency (EPA) for the detection of total phosphorus is colorimetric and based upon the color of a phosphomolybdate complex formed as a result of the reaction between orthophosphates and molybdates ions where ascorbic acid and antimony potassium tartrate are added and serve as reducing agents. Prior to the measurements, all forms of phosphorus are converted into orthophosphates via sample digestion (heating and acidifying). The work presented here details an electrochemical adaptation of this EPA recommended colorimetric approach for the measurement of dissolved phosphorus in water samples using screen-printed graphite macroelectrodes for the first time. This novel indirect electrochemical sensing protocol allows the determination of orthophosphates over the range from 0.5 to 20 μg L(-1) in ideal pH 1 solutions utilizing cyclic voltammetry with a limit of detection (3σ) found to correspond to 0.3 μg L(-1) of phosphorus. The reaction time and influence of foreign ions (potential interferents) upon this electroanalytical protocol was also investigated, where it was found that a reaction time of 5 min, which is essential in the standard colorimetric approach, is not required in the new proposed electrochemically adapted protocol. The proposed electrochemical method was independently validated through the quantification of orthophosphates and total dissolved phosphorus in polluted water samples (canal water samples) with ion chromatography and ICP-OES, respectively. This novel electrochemical protocol exhibits advantages over the established EPA recommended colorimetric determination for total phosphorus with lower detection limits and shorter experimental times. Additionally this electrochemical adaptation allows the determination of dissolved phosphorus without the use of ascorbic acid and antimony potassium tartrate as reducing agents (as used in the colorimetric method). The potential portability of this protocol is demonstrated in the development of the PhosQuant electrochemical device and provides a portable device for the rapid electrochemical detection of dissolved phosphorus using screen-printed electrodes.

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“…Interest in the development of phosphate biosensors for use in different related fields such as biological, environmental, food and agricultural has been continuing since the first report published by Guilbault and Nanjo in 1975 [7,10]. Although researchers have developed singleenzyme biosensors [9,[11][12][13], bi-enzyme biosensors [14][15][16][17], tri enzyme biosensors [18,19] for the over four decades, the simple, cost effective, more efficient and preferred one is pyruvate oxidase based single-enzyme phosphate biosensors [9]. Pyruvate oxidase catalyzes the oxidative decarboxylation of pyruvate, phosphate and oxygen to acetyl phosphate, carbon dioxide and hydrogen peroxide in the presence of cofactors such as flavin adenine dinucleotide (FAD), thiamine pyrophosphate chloride (TPP) and magnesium (Mg + 2 ).…”

Section: Introductionmentioning

confidence: 99%

A Novel Flow‐injection Rhodium Nanoparticles Modified Phosphate Biosensor and its Operation in Artificial Urine

Kilic

2021

Electroanalysis

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A biosensor for phosphate determination with the flow‐injection system was developed using rhodium nanoparticles modified Poly(pyrrole‐co‐[1‐(2‐aminophenyl) pyrrole])/pyruvate oxidase. The biosensor showed a very wide linearity up to 70 mM phosphate concentration compared to previously reports, response time of 4 s., operational stability with a relative standard deviation of 0.009 % and accuracy of 99.4 %±0.949 at a flow rate of 2.0 Ml min.−1 at exactly −0.68 V. Detection limit were calculated to be 21±0.001 μM by preserving 81.1 % of its initial response at the end of 16th days. Artificial urine was analyzed without dilution to investigate biosensor performance.

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Phosphate-sensitive enzyme electrode: a potential sensor for environment control

Cited by 55 publications

References 11 publications

Current research activity in biosensors

Current research activity in biosensors

Rapid and Portable Electrochemical Quantification of Phosphorus

A Novel Flow‐injection Rhodium Nanoparticles Modified Phosphate Biosensor and its Operation in Artificial Urine

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