Phosphate Sensing by Fluorescent Reporter Proteins Embedded in Polyacrylamide Nanoparticles

Sun, Honghao; Scharff-Poulsen, Anne Marie; Gu, Hongchen; Jakobsen, Iver; Koßmann, Jens; Frommer, Wolf B.; Almdal, Kristoffer

doi:10.1021/nn700166x

Cited by 45 publications

(30 citation statements)

References 29 publications

(62 reference statements)

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“…30 A study of the role of the monomer loading and composition of the surfactant revealed that the size of the water nanodroplet in the microemulsion before polymerization is extremely dependent on monomer concentration. 167 Along with some observations on the obtained sizes of nanoparticles, this serves as an indication that the microemulsion is not always stable throughout the synthesis. In several instances, particles that are much larger than the droplet size in the microemulsion or even with bimodal size distributions were obtained.…”

Section: Chemical Reviewsmentioning

confidence: 79%

Facing the Design Challenges of Particle-Based Nanosensors for Metabolite Quantification in Living Cells

et al. 2015

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Section: Chemical Reviewsmentioning

confidence: 79%

Facing the Design Challenges of Particle-Based Nanosensors for Metabolite Quantification in Living Cells

et al. 2015

Self Cite

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“…1,18 The first step in establishing the usefulness of an aptamer-based nanosensor was to synthesize nanoparticles with aptamer switch probes trapped inside. The polyacrylamide particles were synthesized by inverse microemulsion polymerization by mixing aptamer switch probes (ATP-ASP or control-ASP) with acrylamide monomers before the start of polymerization.…”

Section: Resultsmentioning

confidence: 99%

Aptamers Embedded in Polyacrylamide Nanoparticles: A Tool for in Vivo Metabolite Sensing

2010

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We describe a new type of aptamer-based optical nanosensor which uses the embedding of target responsive oligonucleotides in porous polyacrylamide nanoparticles to eliminate nuclease instability. The latter is a common problem in the use of aptamer sensors in biological environments. These aptamers embedded in nanoparticles (AptaNPs) are proposed as a tool in real-time metabolite measurements in living cells. The AptaNPs comprise 30 nm polyacrylamide nanoparticles, prepared by inverse microemulsion polymerization, which contain water-soluble aptamer switch probes (ASPs) trapped in the porous matrix of the nanoparticles. The matrix acts as a molecular fence allowing rapid diffusion of small metabolites into the particles to interact with the aptamer molecules, but at the same time it retains the larger aptamer molecules inside the nanoparticles providing protection against intracellular degradation. We tested the ability of the AptaNPs to measure the adenine-nucleotide content in yeast cells. Our results successfully demonstrate the potential for monitoring any metabolite of interest in living cells by selecting specific aptamers and embedding them in nanoparticles.

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“…Recently, a system using NMR or positron emission tomography provided very useful information, but would be even more difficult to apply in practice (De Smet et al 2012). Another promising development is in vivo phosphate tracking by fluorescent reporter proteins (Gu et al 2006;Sun et al 2008;review: Frommer et al 2009), but this will also not be easily implemented in a high-throughput screening system.…”

Section: Hydroponics: Growing Plants On Water Solutionsmentioning

confidence: 99%

Improving phosphorus use efficiency in agriculture: opportunities for breeding

Wiel¹,

Linden²,

Scholten³

2015

Euphytica

176

105

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Phosphorus (P) is often an important limiting factor for crop yields, but rock phosphate as fertilizer is a non-renewable resource and expected to become scarce in the future. High P input levels in agriculture have led to environmental problems. One of the ways to tackle these issues simultaneously is improving phosphorus use efficiency (PUE) of the crops through breeding. In this review, we describe plant architectural and physiological traits important for PUE. Subsequently, we discuss efficient methods of screening for PUE traits. We address targeted cultivation methods, including solid and hydroponic systems, as well as testing methods, such as image analysis systems, and biomass and photosynthesis measurements. Genetic variation for PUE traits has been assessed in many crops, and genetics of PUE has been studied by quantitative trait loci (QTL) analyses and genome-wide association study. A number of genes involved in the plant's response to low P have been characterized. These genes include transcription factors, and genes involved in signal transduction, hormonal pathways, sugar signalling, P saving metabolic pathways, and in P scavenging, including transporters and metabolites and/or ATP-ases mobilizing P in the soil. In addition, the role of microorganisms promoting PUE of plants, particularly arbuscular mycorrhizal fungi is discussed. An overview is given of methods for selecting for optimal combinations of plant and fungal genotypes, and their genetics, incl. QTLs and genes involved. In conclusion, significant progress has been made in selecting for traits for PUE, developing systems for the difficult but highly relevant root phenotyping, and in identifying QTLs and genes involved.

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Phosphate Sensing by Fluorescent Reporter Proteins Embedded in Polyacrylamide Nanoparticles

Cited by 45 publications

References 29 publications

Facing the Design Challenges of Particle-Based Nanosensors for Metabolite Quantification in Living Cells

Facing the Design Challenges of Particle-Based Nanosensors for Metabolite Quantification in Living Cells

Aptamers Embedded in Polyacrylamide Nanoparticles: A Tool for in Vivo Metabolite Sensing

Improving phosphorus use efficiency in agriculture: opportunities for breeding

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