Phosphate, phytate and phytases in plants: from fundamental knowledge gained in Arabidopsis to potential biotechnological applications in wheat

Secco, David; Bouain, Nadia; Rouached, Aïda; Prom‐u‐thai, Chanakan; Hanin, Moez; Pandey, Ajay Kumar; Rouached, Hatem

doi:10.1080/07388551.2016.1268089

Cited by 53 publications

(46 citation statements)

References 132 publications

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“…[49][50][51][52] Furthermore, having insighti nto the molecular details of the mechanism by whichp lant PAPs hydrolyze substrates will also contribute to the field of plant biotechnology,g uiding the designo ft ransgenic plants that express more efficient PAPs in ordert oi mprovec rop yields and reduce or eliminate the need for fertilizers. [53][54][55][56] …”

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confidence: 99%

The Binding Mode of an ADP Analogue to a Metallohydrolase Mimics the Likely Transition State

et al. 2019

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Purple acid phosphatases( PAPs) are members of the large family of metallohydrolases, ag roup of enzymes that perform aw ide range of biological functions, while employing ah ighly conserved catalytic mechanism. PAPs are found in plants,a nimals and fungi;i nh umans they play an important role in bone turnovera nd are thus of interest for developingt reatments for osteoporosis. The majority of metallohydrolases use am etalbound hydroxide to initiate catalysis, which leads to the formation of ap roposed five-coordinate oxyphosphorane species in the transition state. In this work, we crystallized PAPf rom red kidney beans (rkbPAP) in the presence of both adenosine and vanadate. The in crystallo-formed vanadate analogue of ADP provides detailed insight into the binding mode of aP AP substrate, captured in as tructure that mimics the putative fivecoordinate transition state. Our observations not only provide unprecedented insightinto the mechanism of metallohydrolases, but might also guide the structure-based design of inhibitors for application in the treatment of severalhuman illnesses.Metallohydrolases form al arge familyo fm ostly bimetallic enzymes that use metal ionst oactivate both the nucleophile and scissile bond of various phosphate or amide substrates. [1][2][3][4] Examples include the antibiotic-degrading metallo-b-lactamases, pesticide-decontaminating organophosphate hydrolases, and purple acid phosphatases (PAPs). [5][6][7] PAPs, the only known metallohydrolases that require ah eterovalent Fe III M II center (with M = Fe, Zn or Mn) for catalytic activity, have been characterized from various mammals, plants and fungi. [2,5] Mostp lant PAPs use either Zn II or Mn II ,w hereas their mammalianc ounterparts employ ar edox-activei ron ( Figure 1). [8,9] The reported substrates for PAPs include adenosine 5'-triphosphate (ATP), adenosine 5'-diphosphate( ADP), phosphotyrosine and pyrophosphate. [5,10] The synthetic substrate para-nitrophenyl phosphate (pNPP) is frequently used for functionalstudies. [2,5] The biological functions of PAPs are diverse and dependent on the organism. For mammals, experiments with transgenic mice have demonstrated that PAPp lays an important role in bone metabolism. [11,12] In plants, PAPs playamajor role in the acquisition of phosphorus, especially if there is al imited supplyo fp hosphate. [13,14] Due to their diverse roles, PAPs have attracted attention either as targetsf or new treatments of osteoporosis or for applicationsi na griculture to aid nutrient uptake by crops. [11,15] PAPs operate optimally in the pH range between5 .0 and 6.5, and are characterized by ad istinct purple color due to ac harge-transfer transition between an active-site tyrosine ligand and an Fe III ion. [16,17] The crystal structures of several mammalian and plant PAPs have demonstrated that, despite the difference in the metal ion selectivity and the limited degree of sequence similarity,t heir actives ite geometries are highly conserved (Figure1). [18][19][20][21][22][23] Consequently,t he proposed mod...

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The Binding Mode of an ADP Analogue to a Metallohydrolase Mimics the Likely Transition State

et al. 2019

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“…P is a component of vital biological molecules including nucleic acids, energy sources, phospholipids, and phytic acid [11], and is also involved in the photosynthetic activity [11]. As a result, the content of P is in the range of 0.43%–0.83% of rice dry matter [12], and P starvation results in plants with stunted growth.…”

Section: Introductionmentioning

confidence: 99%

Phosphorus and Iron Deficiencies Influences Rice Shoot Growth in an Oxygen Dependent Manner: Insight from Upland and Lowland Rice

Mongon

Chaiwong

Bouain

et al. 2017

IJMS

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Abstract:Rice is the main staple crop for one-third of the world population. To maximize yields, large quantities and constant input of fertilizers containing essential nutrients such as phosphorus (P) and iron (Fe) are added. Rice can germinate in both aerobic and anaerobic conditions, but the crosstalk between oxygen (O 2 ) and nutrients such as P and Fe on plant growth remains obscure. The aim of this work was to test whether such interactions exist, and, if so, if they are conserved between up-and lowland rice varieties. To do so, we assessed shoot and root biomass as well as inorganic phosphate (Pi) accumulation in four rice varieties, including two lowland rice varieties Nipponbare and Suphanburi 1 (SPR1) (adapted to non-aerated condition) and two upland rice varieties CMU122 and Sew Mae Jun (SMJ) (adapted to aerated condition) under various conditions of Pi and/or Fe deficiencies, in aerated and non-areated solution. Under these different experimental conditions, our results revealed that the altered shoot biomass in Nipponbare and SPR1 was O 2 -dependent but to a lesser extent in CMU122 and SMJ cultivars. In this perspective, discovering the biological significance and molecular basis of these mineral elements and O 2 signal interaction is needed to fully appreciate the performance of plants to multiple environmental changes.

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“…Reducing PA in grains may provide a dual gain with less grain P loss and more micronutrient retention [93,94]. Generation of low PA crops may be achieved by targeting PA biosynthesis genes or transport [29,[95][96][97][98]. As an alternative strategy to achieve low PA grains, roles for other families of transporters are also emerging.…”

Section: Phosphate In Seedsmentioning

confidence: 99%

“…Among these molecular players, the mutation of both PHT1;1 and PHT1;4 or PHO1 and PHO1;H1 causes the most severe phenotype that is characterized by a decreased Pi accumulation in Arabidopsis [19,28], demonstrating the importance of these Pi transporters and exporters in Pi uptake and Pi translocation to shoots. For more information, readers are referred to these reviews [25,29].…”

Section: Phosphate Uptake and Transport In Wheat And Arabidopsismentioning

confidence: 99%

“…In Arabidopsis, decades of research eventually identified the Pi starvation signalling pathway, defined as SPX1-PHR1-miR399-PHO2-PHT1/PHO1 (for review: [29,69]). Key genes encoding for SPX proteins was initially reported to be upregulated in response to P deficiency via transcriptome analysis ( [70]).…”

Section: Phosphate Sensing and Signalling In Arabidopsis And Wheatmentioning

confidence: 99%

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Phosphorus Transport in Arabidopsis and Wheat: Emerging Strategies to Improve P Pool in Seeds

et al. 2018

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Phosphorus (P) is an essential macronutrient for plants to complete their life cycle. P taken up from the soil by the roots is transported to the rest of the plant and ultimately stored in seeds. This stored P is used during germination to sustain the nutritional demands of the growing seedling in the absence of a developed root system. Nevertheless, P deficiency, an increasing global issue, greatly decreases the vigour of afflicted seeds. To combat P deficiency, current crop production methods rely on heavy P fertilizer application, an unsustainable practice in light of a speculated decrease in worldwide P stocks. Therefore, the overall goal in optimizing P usage for agricultural purposes is both to decrease our dependency on P fertilizers and enhance the P-use efficiency in plants. Achieving this goal requires a robust understanding of how plants regulate inorganic phosphate (Pi) transport, during vegetative growth as well as the reproductive stages of development. In this short review, we present the current knowledge on Pi transport in the model plant Arabidopsis thaliana and apply the information towards the economically important cereal crop wheat. We highlight the importance of developing our knowledge on the regulation of these plants' P transport systems and P accumulation in seeds due to its involvement in maintaining their vigour and nutritional quality. We additionally discuss further discoveries in the subjects this review discusses substantiate this importance in their practical applications for practical food security and geopolitical applications.

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Phosphate, phytate and phytases in plants: from fundamental knowledge gained in Arabidopsis to potential biotechnological applications in wheat

Cited by 53 publications

References 132 publications

The Binding Mode of an ADP Analogue to a Metallohydrolase Mimics the Likely Transition State

The Binding Mode of an ADP Analogue to a Metallohydrolase Mimics the Likely Transition State

Phosphorus and Iron Deficiencies Influences Rice Shoot Growth in an Oxygen Dependent Manner: Insight from Upland and Lowland Rice

Phosphorus Transport in Arabidopsis and Wheat: Emerging Strategies to Improve P Pool in Seeds

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