Phosphate-Modified Nucleotides for Monitoring Enzyme Activity

Ermert, Susanne; Marx, Andreas; Hacker, Stephan M.

doi:10.1007/978-3-319-60357-5_6

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2020

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“…The conversion of 18 and 19 into their parent natural MSN was achieved by applying an aqueous solution of 18 and 19 to a Dowex-H + plug filtration. This slightly acidic environment smoothly induced P–N-bond hydrolysis, 39 affording pppApp ( 4 ) and pppGpp ( 3 ). After precipitation, 3 and 4 were isolated in total yields of 26% and 18% with respect to the starting materials 7 and 8 after eight and nine steps and only two intermediates that required purification.…”

Section: Resultsmentioning

confidence: 99%

Four Phosphates at One Blow: Access to Pentaphosphorylated Magic Spot Nucleotides and Their Analysis by Capillary Electrophoresis

et al. 2020

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The complex phosphorylation pattern of natural and modified pentaphosphorylated magic spot nucleotides is generated in a highly efficient way. A cyclic pyrophosphoryl phosphoramidite (cPyPA) reagent is used to introduce four phosphates on nucleosides regioselectively in a one-flask key transformation. The obtained magic spot nucleotides are used to develop a capillary electrophoresis UV detection method, enabling nucleotide assignment in complex bacterial extracts.

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Section: Resultsmentioning

confidence: 99%