Phosphate Glass as a Phosphate Source in High Cell Density Escherichia coli Fermentations

Curless, C.; Baclaski, J.; Sachdev, R.

doi:10.1021/bp960224e

Cited by 2 publications

(2 citation statements)

References 6 publications

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“…Large-scale cultures were performed in a 5 L fermenter (Labfors III, Infors AG) using a glycerol fed-batch strategy to accumulate biomass prior to induction with methanol (8), with sodium hexametaphosphate added as the phosphate source (9).…”

Section: Methodsmentioning

confidence: 99%

Molecular Properties of Kcv, a Virus Encoded K⁺ Channel

et al. 2007

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The miniature viral K+ channel Kcv represents the pore module of all K+ channels. A synthetic gene of Kcv with an elevated GC content compared to that of the wild-type gene was expressed heterologously in Pichia pastoris, and the purified protein was functionally reconstituted into liposomes. Biochemical assays reveal a remarkable cation selctive stability of the channel tetramer via SDS−PAGE. Only cations, which permeate Kcv, were able to protect the oligomer against disassembly into monomers at high temperatures. Electrophysiological characterization of the single Kcv channel reveals a saturating conductance (Λmax) of 360 pS; the single-channel current−voltage relation was strongly rectifying with a negative slope conductance at extreme voltages. The channel was highly selective for K+ and was blocked by Ba2+ and in a side specific manner by Na+ and Cs+ also. The channel conducted Rb+, but as a consequence, the channel was shifted into a hyperactive state. We conclude that specific binding interactions of cations in the conductive pathway are an important determinant of channel stability and function.

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Section: Methodsmentioning

confidence: 99%

Molecular Properties of Kcv, a Virus Encoded K⁺ Channel

et al. 2007

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“…In fermentation perspective, usage of polyphosphate exhibits marked advantages viz. polyphosphate costs no more than sodium or potassium orthophosphate, is more soluble than any other inorganic phosphate source, and does not form metallo‐phosphate precipitates when mixed with other nutrients in proportions found in fermentation media [12].…”

Section: Resultsmentioning

confidence: 99%

Maximization of recombinantHelicobacter pylorineutrophil activating protein production inEscherichia coli: improvement of a chemically defined medium using response surface methodology

Niccolai

Fontani

Kapat

et al. 2003

FEMS Microbiology Letters

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Medium optimization for the production of constitutive recombinant Helicobacter pylori neutrophil activating protein (NAP) in Escherichia coli was investigated by using response surface methodology. Carbon to nitrogen ratio, concentrations of sodium polyphosphate and magnesium sulfate were considered as independent variables. The optimized medium was a chemically defined medium with a carbon to nitrogen ratio of 14.4 and with concentrations of sodium polyphosphate and magnesium sulfate about 7.1 g l(-1) and 3.04 g l(-1) respectively. The maximum recombinant NAP production level (1184.6 mg l(-1)) was 29.96% higher than that in control medium.

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Phosphate Glass as a Phosphate Source in High Cell Density Escherichia coli Fermentations

Cited by 2 publications

References 6 publications

Molecular Properties of Kcv, a Virus Encoded K⁺ Channel

Molecular Properties of Kcv, a Virus Encoded K⁺ Channel

Maximization of recombinantHelicobacter pylorineutrophil activating protein production inEscherichia coli: improvement of a chemically defined medium using response surface methodology

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Phosphate Glass as a Phosphate Source in High Cell Density Escherichia coli Fermentations

Cited by 2 publications

References 6 publications

Molecular Properties of Kcv, a Virus Encoded K+ Channel

Molecular Properties of Kcv, a Virus Encoded K+ Channel

Maximization of recombinantHelicobacter pylorineutrophil activating protein production inEscherichia coli: improvement of a chemically defined medium using response surface methodology

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Product

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Molecular Properties of Kcv, a Virus Encoded K⁺ Channel

Molecular Properties of Kcv, a Virus Encoded K⁺ Channel