Phosphate directed Y-M variation in candida albicans

Widra, A.

doi:10.1007/bf02068455

Cited by 28 publications

(13 citation statements)

References 15 publications

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“…Stationary-phase phenotype. The role of zinc in the growth and dimorphism of the infectious yeast Candida albicans has become a renewed topic of interest (Soll, in press), primarily because of its effects on the phenotype of stationary-phase cells (2,15,20,21;Soll,in press). When cells enter stationary phase due to the depletion of zinc, they are predominantly unbudded and relatively homogeneous in volume.…”

Section: Discussionmentioning

confidence: 99%

Effects of zinc on stationary-phase phenotype and macromolecular synthesis accompanying outgrowth of Candida albicans

Anderson¹,

Soll²

1984

Infect Immun

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When cultures of Candida albicans which had entered stationary phase due to the depletion of zinc (zinclimiting conditions) were compared with cultures which had entered stationary phase due to the depletion of another growth-limiting component (zinc-excess conditions), at least two cellular characteristics were found to differ: (i) zinc-limited cells appeared more homogeneous and larger on the average than zinc-excess cells, and (ii) zinc-limited cells evaginated on the average 40 min later than zinc-excess cells. In the present study, it is demonstrated that (i) the distribution of volumes for a stationary-phase culture of zinc-excess cells is skewed towards very small volumes, but even the smallest cells contain nuclei; in contrast, the volumes of zinc-limited cells are evenly distributed around a much larger mean value; (ii) the evagination kinetics of zinc-excess cells released into fresh medium are far less synchronous than are those of zinc-limited cells, and the smaller cells in the population take much longer to evaginate than do the larger cells; (iii) the onset of net RNA accumulation and achievement of a maximum rate of [3H]uridine incorporation occur significantly earlier in zinc-excess cells than in zinc-limited cells released into fresh medium; and (iv) the onset of net protein accumulation and [3H]leucine incorporation occur significantly earlier in zinc-excess cells than in zinc-limited cells released into fresh medium. These results indicate that although zinc-excess cells are extremely heterogeneous in volume, they may still be homogeneously blocked in the nuclear division cycle, and that the later average evagination time of released zinc-limited cells may be due to a delay in the onset of protein synthesis, which in turn may be due to the time necessary to reaccumulate zinc to levels sufficient for the reinitiation of RNA synthesis.

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Section: Discussionmentioning

confidence: 99%

Effects of zinc on stationary-phase phenotype and macromolecular synthesis accompanying outgrowth of Candida albicans

Anderson¹,

Soll²

1984

Infect Immun

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“…Media were devised based on those of Nickerson & Mankowski (1g53), Roth & Goldstein (1961) and Widra (1964); for blastospore production medium A containing glucose (0.2 %), sodium chloride (0.3 %), Difco yeast extract (0.1 %) and Difco Bactopeptone (5.0 yo) with incubation at 37' , and medium B as medium A but substituting Difco Neopeptone for the Bactopeptone and starch for glucose with incubation at 30". Mycelial phase was grown on medium B with incubation at 40" and on IOO % ox serum (medium C ) with incubation at 37'.…”

Section: Organismmentioning

confidence: 99%

Cell Wall Composition of the Mycelial and Blastospore Forms of Candida albicans

Chattaway

Holmes

Barlow

1968

Journal of General Microbiology

272

120

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S U M M A R YCell walls were obtained from the mycelial and blastospore forms of Candida albicans ; these were delipidized and separated into alkali-soluble and alkali-insoluble fractions. The detailed composition of these fractions was determined in organisms grown on different media at 37' and on the same medium at different temperatures (blastospores 30°, mycelium 40'). The composition of the wall of each form was found to be constant, irrespective of growth conditions, except for some variation in the amounts of mannose and glucose in wall hydrolysates. The alkali-insoluble fraction from the mycelial form contained 3 times as much chitin as that from blastospores and only about one third as much protein. The protein from these two fractions showed marked differences in amino acid composition. Differences between the two morphological forms in the amounts of carbohydrate and protein in the alkali-soluble fractions were also found. The results are discussed in relation to other studies of cell-wall composition in the dimorphic fungi.

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“…Candida albicans grows either as a budding yeast or as an elongating mycelium. It has been reported that micromolar concentrations of the divalent cation zinc suppress the formation of the mycelial form in vitro (11,12). In addition, a depression of the in vivo level of zinc has been implicated in Candida pathogenesis in individuals suffering from acrodermatitis enteropathica, a disease related to zinc deficiency (2).…”

mentioning

confidence: 99%

Effects of low concentrations of zinc on the growth and dimorphism of Candida albicans: evidence for zinc-resistant and -sensitive pathways for mycelium formation

Bedell¹,

Soll²

1979

Infect Immun

173

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In this analysis we have examined in detail the effects of low concentrations of zinc on the growth and dimorphism of Candida albicans. Evidence is presented that micromolar concentrations of zinc added to growth cultures grown at 250C (i) cause a twofold increase in the final concentration of spheres at stationary phase, (ii) result in an asynchronous block in the budding cycle at stationary phase, (iii) completely suppress mycelium formation in two independently isolated human strains which produce low but significant levels of mycelia at stationary phase, and (iv) completely suppress mycelium formation in cultures of mutant M10, in which over 60% of the cells form mycelia at stationary phase. In contrast, micromolar concentrations of zinc do not inhibit mycelium formation induced by releasing cells from stationary-phase cultures into fresh medium at 370C. In addition, if zinc is present in the growth medium of the initial culture at 250C, the average time of subsequent mycelium formation after release into fresh medium at 370C is halved. It is demonstrated that the above effects are specific to zinc.

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Phosphate directed Y-M variation in candida albicans

Cited by 28 publications

References 15 publications

Effects of zinc on stationary-phase phenotype and macromolecular synthesis accompanying outgrowth of Candida albicans

Effects of zinc on stationary-phase phenotype and macromolecular synthesis accompanying outgrowth of Candida albicans

Cell Wall Composition of the Mycelial and Blastospore Forms of Candida albicans

Effects of low concentrations of zinc on the growth and dimorphism of Candida albicans: evidence for zinc-resistant and -sensitive pathways for mycelium formation

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