Phosphatase activity in anaerobic bioreactors for wastewater treatment

Anupama, V. N.; Amrutha, P.N.; Chitra, G.; Krishnakumar, B.

doi:10.1016/j.watres.2008.02.014

Cited by 42 publications

(16 citation statements)

References 35 publications

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“…In cucumber rhizosphere soil from the control and CSY-F1, FA, and CSY-F1 + FA treatment groups, CAT (Roberge 1978), alkaline phosphatase (Anupama et al 2008), urease (Douglas and Bremner 1971), and sucrase (Schinner and Von Mersi 1990) activities were measured. Since CSY-F3 degraded FA poorly, the effects of CSY-F3 on soil enzymes were not investigated.…”

Section: Determination Of Activities Of Soil Enzymesmentioning

confidence: 99%

Bacillus methylotrophicus isolated from the cucumber rhizosphere degrades ferulic acid in soil and affects antioxidant and rhizosphere enzyme activities

et al. 2015

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Aims Ferulic acid (FA) accumulates in soil and inhibits plant growth. We examined whether the FA-degrading bacterium Bacillus methylotrophicus degrades FA in soil. Methods B. methylotrophicus strain CSY-F1 was isolated and applied to unplanted soil and soil planted with Cucumis sativus (cucumber). We analyzed the effects of B. methylotrophicus on rhizosphere enzyme activities and antioxidant enzyme activities in cucumber and CSY-F1 exposed to FA. Results CSY-F1 degraded FA in culture medium and in soil, giving rise to 4-vinyl guaiacol, vanillin, vanillic acid, and protocatechuic acid. When cucumber seedlings were grown in soil treated with FA, the activities of some soil enzymes decreased, and the malonaldehyde content in cucumber leaves increased. The addition of CSY-F1 to FA-treated soil increased the activities of these soil enzymes, decreased the FA concentration in the soil, and elevated the activities of some antioxidant enzymes in seedlings. Moreover, the levels of superoxide radical, hydrogen peroxide, and malonaldehyde were reduced in cucumber leaves. FA treatment increased the activities of antioxidant enzymes, including superoxide dismutase, catalase, and monodehydroascorbate, in CSY-F1. Conclusion Bacillus methylotrophicus CSY-F1 has potential applications as an FA-degrading agent in soil, as it mitigates FA stress in cucumber seedlings by activating several antioxidant and soil enzymes.

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Section: Determination Of Activities Of Soil Enzymesmentioning

confidence: 99%

Bacillus methylotrophicus isolated from the cucumber rhizosphere degrades ferulic acid in soil and affects antioxidant and rhizosphere enzyme activities

et al. 2015

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“…The average APA ranged from 4.17 to 13.77 μmol g -1 h -1 for sewage samples and from 0.82 to 2.96 μmol g -1 h -1 for soils and sediments. Alkaline phosphatases are intracellular enzymes, and their activities have been found to be tightly associated with the biomass (Goel et al 1998;Albiach et al 2000;Anupama et al 2008). In this experiment, the APA in different samples greatly varied, and it is primarily attributed to different biomass levels in the samples.…”

Section: Alkaline Phosphatase Activitymentioning

confidence: 78%

“…The APA assays were adjusted and carried out according to Anupama et al (2008). Two hundred milligrams of soil or sediment samples or 50 mg of sewage sludge sample was made up to 4 mL with Tris-HCl buffer (pH 8.0), and the mixture was sonicated with a compact ultrasonic cleaner (J&L Shanghai Ultrasonic Ltd., JL-60) for 60 s. To this mixture, 1 mL of p-NPP disodium salt (Amresco Inc., USA) was added as a substrate, thoroughly mixed, and incubated at 37°C in a water bath for 1 h. After incubation, 1 mL of 1 M NaOH was added to stop further reaction.…”

Section: Alkaline Phosphatase Activity Assaysmentioning

confidence: 99%

Comparison of phosphorus fractions and alkaline phosphatase activity in sludge, soils, and sediments

et al. 2011

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Purpose The land application or disposal of sewage sludge generally leads to phosphorus (P) loss in aquatic environments and often results in eutrophication. The nature of P fractions plays a key role in the transport of P within the environment, and alkaline phosphatase is important for the transformation of the P fractions. The objective of this study was to assess the P fractions and alkaline phosphatase activity in sewage sludge, soils, and sediments, in order to effectively evaluate their P bioavailability and mobility. Materials and methods The Standards, Measurements and Testing (SMT) harmonized procedure was adopted for the determination of the P fractions. The alkaline phosphatase activities were determined by analyzing the production of p-nitrophenol from p-nitrophenylphosphate catalyzed by phosphatase in the various samples. Results and discussionThe results demonstrated that sewage sludge possessed larger amounts of P than soils and sediments. Inorganic phosphorus (IP) was the main P fraction of all the samples investigated and it always constituted >70% of the total P (TP). However, the distribution of IP between non-apatite inorganic P (NAIP) and apatite P (AP) varied significantly according to the type of sample. NAIP accounted for >60% of IP in sediment and sewage sludge samples obtained from domestic wastewater treatment plants. However, AP was more abundant than NAIP in soil samples. For the P fractions obtained in a closed agriculture test field, NAIP content showed an obvious increase from soil to sediment samples. This suggests that NAIP is the most easily mobilizable form of P. The average alkaline phosphatase activity in sewage sludge samples is significantly higher than that in soil and sediment samples, and it may be closely attributed to the higher biomass content in sewage samples.Conclusions The results showed that the SMT protocol could be considered a useful tool for P monitoring of sewage sludge, soils, and sediments. The NAIP is the most easily mobilizable form of P and should receive further attention. The sewage sludge samples contain higher mobilizable forms of P and alkaline phosphatase activity than soil and sediment samples. The results are not only beneficial for analyzing P in sewage, soil, and sediment samples during their transfers to watercourses but are also beneficial for evaluating the risk of eutrophication and that associated with sewage sludge treatment and application.

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“…Phosphatase enzymes are classified by pH optima (acid or alkaline), molecular weight, and those that hydrolyze only phosphorylated serine or threonine residues [140]. Acid and alkaline phosphatases (either cytoplasmic or periplasmic) are fundamentally required for microbial nutrient acquisition [116,119,[141][142][143].…”

Section: Phosphatase-mediated Biomineralizationmentioning

confidence: 99%

Phosphate-Mediated Remediation of Metals and Radionuclides

Martinez

Beazley

Sobecky

2014

Advances in Ecology

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Worldwide industrialization activities create vast amounts of organic and inorganic waste streams that frequently result in significant soil and groundwater contamination. Metals and radionuclides are of particular concern due to their mobility and longterm persistence in aquatic and terrestrial environments. As the global population increases, the demand for safe, contaminantfree soil and groundwater will increase as will the need for effective and inexpensive remediation strategies. Remediation strategies that include physical and chemical methods (i.e., abiotic) or biological activities have been shown to impede the migration of radionuclide and metal contaminants within soil and groundwater. However, abiotic remediation methods are often too costly owing to the quantities and volumes of soils and/or groundwater requiring treatment. The in situ sequestration of metals and radionuclides mediated by biological activities associated with microbial phosphorus metabolism is a promising and less costly addition to our existing remediation methods. This review highlights the current strategies for abiotic and microbial phosphatemediated techniques for uranium and metal remediation.

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Phosphatase activity in anaerobic bioreactors for wastewater treatment

Cited by 42 publications

References 35 publications

Bacillus methylotrophicus isolated from the cucumber rhizosphere degrades ferulic acid in soil and affects antioxidant and rhizosphere enzyme activities

Bacillus methylotrophicus isolated from the cucumber rhizosphere degrades ferulic acid in soil and affects antioxidant and rhizosphere enzyme activities

Comparison of phosphorus fractions and alkaline phosphatase activity in sludge, soils, and sediments

Phosphate-Mediated Remediation of Metals and Radionuclides

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