Phorbol myristic acetate stimulates LAF production by the macrophage cell line, P388D

Mizel, Steven B.; Rosenstreich, David L.; Oppenheim, Joost J.

doi:10.1016/0008-8749(78)90330-1

Cited by 142 publications

(66 citation statements)

References 4 publications

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“…PFC to SRBC were determined on day 4. The background PFC in cultures without TRF were 28 (24)(25)(26)(27)(28)(29)(30)(31)(32). The geometric means (±SE) of triplicate cultures is shown.…”

Section: B Cell Growth-promoting Activity and Its Assay The Results mentioning

confidence: 99%

Production of a B cell growth-promoting activity, (DL)BCGF, from a cloned T cell line and its assay on the BCL1 B cell tumor.

Swain¹,

Dutton²

1982

The Journal of Experimental Medicine

131

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It has long been clear that there are two components of the B cell response to antigen. There is an early phase of proliferation in which the responding B cell population is expanded many times, and a later phase of differentiation in which the already proliferating B cell moves on to the immunoglobulin-secretion stage. It has been suggested that separate signals are involved in each phase (1); it has also been realized that there must be an initial lag phase (2) before the onset of proliferation; more recent studies (3) have defined this activation step as a separate event.It has been many years since the need for a helper T cell in the B cell response to antigen was defined (4, 5) and since the demonstration that the T cell could be replaced by a helper factor (6).It is now known that a number of T cell-and macrophage-derived nonantigenspecific factors make up the activity previously defined as "helper factor." Current studies are underway to characterize each factor and to determine at which stage it acts and what is its exact role. Parker (7) has shown that B cell proliferation can be seen in cultures of positively selected B cells stimulated with anti-#. He showed that anti-# alone was not sufficient, but that interleukin 2-(IL-2) 1 containing supernatants were also required. Immunoglobulin secretion required an additional factor (or factors) not provided in IL-2, but which were present in the supernatant of concanavalin A-(Con A) stimulated T cells. Howard et al. (8; and M. Howard, personal communication) also showed that a number of factors were required for B cell proliferation. In her analysis, the extent of proliferation to anti-Ig was proportional to the third power of the B cell concentration. The slope of this curve was reduced to two by the addition of an IL-2-containing supernatant from EL4 and to nearly one by the further addition of an 18-mol wt cut from the supernatant of P388D1 (presumably, interleukin 1 [IL-1]). The B cell growth factor (BCGF) activity present in the EL4 supernatants could be separated from the IL2 activity by phenyl Sepharose

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“…PFC to SRBC were determined on day 4. The background PFC in cultures without TRF were 28 (24)(25)(26)(27)(28)(29)(30)(31)(32). The geometric means (±SE) of triplicate cultures is shown.…”

Section: B Cell Growth-promoting Activity and Its Assay The Results mentioning

confidence: 99%

Production of a B cell growth-promoting activity, (DL)BCGF, from a cloned T cell line and its assay on the BCL1 B cell tumor.

Swain¹,

Dutton²

1982

The Journal of Experimental Medicine

131

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“…The factors reported here may correspond to the specific proteins in [2--4]. TPA stimulates the production of interleukins by macrophages (15 000 Mr) [5] and T cells (~30 000Mr) [6]. A similar factor (15-25 000 Mr) was found in murine keratinocyte which stimulates PHA-dependent thymocyte DNA synthesis [8].…”

Section: Resultsmentioning

confidence: 70%

“…However, the biological activities of these proteins have not been elucidated. There are several reports that TPA stimulates lymphoid cells to produce various factors which regulate immune responses: interleukin 1 and 2 [5,6]. Thus, TPA-treated fibroblast cells may produce factors which influence immune responses.…”

Section: Introductionmentioning

confidence: 99%

3T3 fibroblasts are stimulated by 12‐O‐tetradecanoyl‐phorbol‐13‐acetate to produce thymocyte‐activating factors

1982

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“…In contrast, lectin-stimulated peripheral blood lymphocytes (PBL) showed the activities at 60-100,20 and lo-15 kDa (open circles in fig.1). Since the assay method mentioned above reflects the various T cell growth activities such as interleukin 1 (IL 1) [9] and 2 (IL 2) [4], IL 2 activities in these T cell DNA synthetic activities were analyzed with an IL 2-dependent killer T cell clone. The major IL 2 activities of MT 1 cells were detected at 60-100 kDa and lesser activities were observed at 30 and lo-15 kDa (closed circles in fig.2).…”

Section: Resultsmentioning

confidence: 99%

T cell growth factors from adult T cell leukemia virus‐transformed cell lines

et al. 1984

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Some characteristics of T cell growth factors derived from adult T cell leukemia virus (ATLV)-transformed cell lines, MT I and MT 2 were analyzed. MT 1 cells release significant interleukin 2 (IL 2) activity into the culture medium, which showed the same elution pattern of gel filtration and isoelectric focusing of IL 2 from lectin-stimulated normal human lymphocytes. This activity was also detected in the cell extract of MT 1. In contrast, MT 2 cell line did not produce IL 2 activity, but non-IL 2 type growth factor was observed. The significance of these factors from MT cell lines is discussed from the viewpoint of 'autokine' in ATLV-transformed cells. ALTV-transformed cell

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Phorbol myristic acetate stimulates LAF production by the macrophage cell line, P388D

Cited by 142 publications

References 4 publications

Production of a B cell growth-promoting activity, (DL)BCGF, from a cloned T cell line and its assay on the BCL1 B cell tumor.

Production of a B cell growth-promoting activity, (DL)BCGF, from a cloned T cell line and its assay on the BCL1 B cell tumor.

3T3 fibroblasts are stimulated by 12‐O‐tetradecanoyl‐phorbol‐13‐acetate to produce thymocyte‐activating factors

T cell growth factors from adult T cell leukemia virus‐transformed cell lines

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