phoP maintains the environmental persistence and virulence of pathogenic bacteria in mechanically stressed desiccated droplets

Hariharan, Vishnu; Chowdhury, Atish Roy; S, Srinivas Rao; Chakravortty, Dipshikha; Basu, Saptarshi

doi:10.1016/j.isci.2023.106580

Cited by 6 publications

(7 citation statements)

References 69 publications

(81 reference statements)

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“…This difference is significant in straight channel compared to serpentine channels. Similar trends have been previously reported by our group for Salmonella Typhimurium subjected to mechanical stresses 68 . Ares et al 78 reported the importance of cholesterol in reducing the anti-phagocytic properties of KP capsules.…”

Section: Introductionsupporting

confidence: 92%

“…Protection from osmotic stresses by bacteria was studied by Wang et al 67 where they highlighted the role of hydrated bacterial capsule in dampening the osmotic stresses. Recently, Hariharan et al 68 studied the effect of impact stresses on bacterial surfaces and found significant surface damage with spikes and bumps all over the cell. The morphological changes obtained in present study are non-trivial and hence demands a deeper investigation of the bacterial strain at genetic level to understand the exact reason for such alterations.…”

Section: Introductionmentioning

confidence: 99%

“…It has been seen that under various kinds of stress conditions like osmotic stress 71 , thermal stress 71 , oxidative stress 72 , shear stress 73 , evaporative and impact stress 68 results in a loss of viability of the bacterial population. We conduct different tests by keeping flowrate and geometry constant in each one of them to understand how the viability of bacterial cells changes.…”

Section: Introductionmentioning

confidence: 99%

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KlebsiellaPneumoniae turns more virulent under flow stresses in capillary like microchannels

Jain,

Singh,

Tiwari

et al. 2023

Preprint

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Fluidic habitats are very common to bacterial life, however, very little is known about the effect of the flow stresses on the virulence of the bacteria. In the present work, we conduct microfluidic experiments to understand the consequence of stresses generated by flowing fluid on the bacterial morphology and virulence. We consider Klebsiella pneumoniae (KP), an ESKAPE pathogen as the model bacteria that are responsible for blood stream infections like bacteremia apart from pneumonia, urinary tract infections and more. We generate four different stress conditions by changing the flow rate and channel geometry subsequently altering the shear rate and stressing time (T). We observe significant changes in the structural aspects of the stressed bacteria. With an increase in stressing parameters, the viability of the bacterial sample deteriorated. Most importantly, these stressed samples proliferate much more than unstressed samples inside the RAW264.7 murine macrophages. The results shed light on the complex relationship between flow stresses and bacterial virulence. Furthermore, we challenge the bacterial samples with ciprofloxacin to see how they behave under different stress conditions. The present study can be extended to model deadly diseases like bacteremia using organ-on-a-chip technology and help understand bacterial pathogenicity under realistic environments.

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Section: Introductionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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KlebsiellaPneumoniae turns more virulent under flow stresses in capillary like microchannels

Jain,

Singh,

Tiwari

et al. 2023

Preprint

Self Cite

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“…67 Interestingly, there can be bacteria that are alive but not culturable. 68 It has been shown that various stress conditions like osmotic stress, 69 thermal stress, 69 oxidative stress, 70 shear stress, 71 evaporative and impact stress 72 result in a loss of viability of the bacterial population. We conduct different tests by maintaining the flow rate and geometry constant in each one of them to understand how the viability of bacterial cells changes.…”

Section: Viability Assaymentioning

confidence: 99%

Observations on phenomenological changes in Klebsiella Pneumoniae under fluidic stresses

Jain,

Singh,

Tiwari

et al. 2023

Soft Matter

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“…Owing to the excessive use of antibiotics, the rapid emergence of multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains of S. Typhi made Salmonella infection extremely difficult to treat . To study the in vivo pathogenesis of S. Typhimurium, many susceptible mouse strains, such as BALB/c and C57BL/6, mimicking the symptoms of typhoid fever in humans are extensively used. , However, very few suitable models are available to study the in vivo pathogenesis and disease progression of S. Typhi. Additionally, a reduction in the size of its functional genome by either pseudogene formation or the absence of specific genes made our understanding of typhoid fever extremely difficult.…”

Section: Introductionmentioning

confidence: 99%

From Eberthella typhi to Salmonella Typhi: The Fascinating Journey of the Virulence and Pathogenicity of Salmonella Typhi

et al. 2023

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Salmonella Typhi (S. Typhi), the invasive typhoidal serovar of Salmonella enterica that causes typhoid fever in humans, is a severe threat to global health. It is one of the major causes of high morbidity and mortality in developing countries. According to recent WHO estimates, approximately 11–21 million typhoid fever illnesses occur annually worldwide, accounting for 0.12–0.16 million deaths. Salmonella infection can spread to healthy individuals by the consumption of contaminated food and water. Typhoid fever in humans sometimes is accompanied by several other critical extraintestinal complications related to the central nervous system, cardiovascular system, pulmonary system, and hepatobiliary system. Salmonella Pathogenicity Island-1 and Salmonella Pathogenicity Island-2 are the two genomic segments containing genes encoding virulent factors that regulate its invasion and systemic pathogenesis. This Review aims to shed light on a comparative analysis of the virulence and pathogenesis of the typhoidal and nontyphoidal serovars of S. enterica.

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phoP maintains the environmental persistence and virulence of pathogenic bacteria in mechanically stressed desiccated droplets

Cited by 6 publications

References 69 publications

KlebsiellaPneumoniae turns more virulent under flow stresses in capillary like microchannels

KlebsiellaPneumoniae turns more virulent under flow stresses in capillary like microchannels

Observations on phenomenological changes in Klebsiella Pneumoniae under fluidic stresses

From Eberthella typhi to Salmonella Typhi: The Fascinating Journey of the Virulence and Pathogenicity of Salmonella Typhi

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