Phloridzin improves lipoprotein lipase activity in stress-loaded miceviaAMPK phosphorylation

Abstract

Long-term stress exposure can lead to disturbed homeostasis and cause many life-style diseases. Phloridzin possesses various bioactivities, but the understanding of the effects of phloridzin on stress-related lipid metabolism disorder is limited. Our results demonstrate that phloridzin improved plasma lipoprotein lipase (LPL) activity and triglyceride metabolism in restrained mice. A decrease of angiopoietin-like protein 4 (ANGPTL4) mRNA expression and an increase of AMP-activated protein kinase (AMPK) phosphorylation were observed after phloridzin treatment. After inhibiting AMPK phosphorylation, the effects of phloridzin on the amelioration of plasma LPL activity and suppression of ANGPTL4 expression were blocked. In addition, cardiac AMPK phosphorylation, plasma LPL activity and ANGPTL4 expression were also affected by phloridzin, even if the glucocorticoid receptor was blocked. Taken together, the down-regulation of ANGPTL4 expression by phloridzin was probably via a direct activation of AMPK pathway. This discovery can provide a biochemical and nutritional basis for the use of phloridzin-containing food and beverage in daily life.

Autophagic degradation of PML promotes susceptibility to HSV-1 by stress-induced Corticosterone

“…To this end, mice were challenged with restrained stress and/or HSV-1 according to the experimental procedures (Figure 1 A). Consistently with our previous study 40 , the plasma level of CORT, a stress-induced hormone, was increased significantly after restraint stress loading (Figure 1 B). A significantly lowered survival rate was observed in the model group (Stress + HSV-1) as compared with the HSV-1 group (Figure 1 C).…”

The Bioavailability, Extraction, Biosynthesis and Distribution of Natural Dihydrochalcone: Phloridzin

“…Pei et al [101] revealed that phloridzin may be a renal protective agent by regulating differentially expressed proteins, which not only affected glomerular dysfunction and tubular transport, but also played active roles in oxidative stress and lipid metabolism. Wang et al [102] demonstrated that phloridzin increased plasma lipoprotein lipase activity, and improved triglyceride metabolism via the adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) pathway in stress-loaded mice. Wang et al [103] found that extracts from the fruits of Malus baccata contained phloridzin, and the extracts had radioprotective and immunomodulatory activities.…”

Quarter-Century Explorations of Bioactive Polyphenols: Diverse Health Benefits

“…(12) Promoting p53 pathway. Mediated by AMPK activation, curcumin is able to phosphorylate at S15 in p53 N-terminus [28][29][30][31][32][33], which attenuates p53 interaction with its negative regulator MDM2 for promoting p53 stabilization and nuclear translocation for its transcriptional activity. Such action favors cell apoptosis and suppresses cell proliferation.…”

“…(13) LPL upregulation. AMPK phosphorylates and activates LPL [33], a major circulating enzyme responsible for TG-rich lipoprotein catabolism and TG degradation in lowering blood TG level. ( 14) CREBP activation and BDNF expression.…”